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Literature summary extracted from

  • Roje, S.; Chan, S.Y.; Kaplan, F.; Raymond, R.K.; Horne, D.W.; Appling, D.R.; Hanson, A.D.
    Metabolic engineering in yeast demonstrates that S-adenosylmethionine controls flux through the methylenetetrahydrofolate reductase reaction in vivo (2002), J. Biol. Chem., 277, 4056-4061 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.53
-
 Saccharomyces cerevisiae
1.5.1.54
-
 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.53 additional information chromosomal copy of MET13 is replaced by an Arabidopsis thaliana MTHFR cDNA (AtMTHFR-1) or by a chimeric sequence (Chimera-1) comprising the yeast N-terminal domain and the AtMTHFR-1 C-terminal domain. Chimera-1 uses both NADH and NADPH and is insensitive to S-adenosyl-L-methionine. Engineered yeast expressing Chimera-1 accumulates 140fold more S-adenosyl-L-methionine and 7fold more methionine than does the wild-type and grows normally. Yeast expressing AtMTHFR-1 accumulates 8fold more S-adenosyl-L-methionine Saccharomyces cerevisiae
1.5.1.54 additional information chromosomal copy of MET13 is replaced by an Arabidopsis thaliana MTHFR cDNA (AtMTHFR-1) or by a chimeric sequence (Chimera-1) comprising the yeast N-terminal domain and the AtMTHFR-1 C-terminal domain. Chimera-1 uses both NADH and NADPH and is insensitive to S-adenosyl-L-methionine. Engineered yeast expressing Chimera-1 accumulates 140fold more S-adenosyl-L-methionine and 7fold more methionine than does the wild-type and grows normally. Yeast expressing AtMTHFR-1 accumulates 8fold more S-adenosyl-L-methionine Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.53 0.011
-
 5,10-methylenetetrahydrofolate wild-type, cosubstrate NADPH, pH 7.2, temperature not specified in the publication Saccharomyces cerevisiae
1.5.1.53 0.011
-
 NADPH wild-type, pH 7.2, temperature not specified in the publication Saccharomyces cerevisiae
1.5.1.53 0.106
-
 5,10-methylenetetrahydrofolate Chimera-1, cosubstrate NADPH, pH 7.2, temperature not specified in the publication Saccharomyces cerevisiae
1.5.1.53 0.152
-
 5,10-methylenetetrahydrofolate Chimera-1, cosubstrate NADH, pH 7.2, temperature not specified in the publication Saccharomyces cerevisiae
1.5.1.54 0.106
-
 5,10-methylenetetrahydrofolate Chimera-1, cosubstrate NADPH, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
1.5.1.54 0.152
-
 5,10-methylenetetrahydrofolate Chimera-1, cosubstrate NADH, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
1.5.1.54 0.287
-
 5,10-methylenetetrahydrofolate wild-type, cosubstrate NADH, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
1.5.1.54 0.873
-
 NADH wild-type, pH 7.2, temperature not specified in the publication Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.53 Saccharomyces cerevisiae P53128 cf. EC 1.5.1.20
-
1.5.1.54 Arabidopsis thaliana Q9SE60 cf. EC 1.5.1.20
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.53 5,10-methylenetetrahydrofolate + NADH + H+
-
 Saccharomyces cerevisiae 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.53 5,10-methylenetetrahydrofolate + NADPH + H+
-
 Saccharomyces cerevisiae 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.54 5,10-methylenetetrahydrofolate + NADPH + H+
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.53 MET13
-
 Saccharomyces cerevisiae
1.5.1.53 MTHFR
-
 Saccharomyces cerevisiae
1.5.1.54 MTHFR1
-
 Arabidopsis thaliana