Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luo, P.; Dai, S.; Zeng, J.; Duan, J.; Shi, H.; Wang, J.
    Inward-facing conformation of L-ascorbate transporter suggests an elevator mechanism (2018), Cell Discov., 4, 35 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.194 expressed in Escherichia coli C43 (DE3) cells Pasteurella multocida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.194 EIICasc in the inward-facing conformation, hanging drop vapor diffusion method, using 0.28 M CaCl2, 0.1M Tris-HCl, pH 7.5,42% (w/v) PEG400 Pasteurella multocida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.194 [protein]-Npi-phospho-L-histidine + L-ascorbate[side 1] Pasteurella multocida
-
 [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?
2.7.1.194 [protein]-Npi-phospho-L-histidine + L-ascorbate[side 1] Pasteurella multocida Pm70
-
 [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.194 Pasteurella multocida Q9CMQ1
-
-
2.7.1.194 Pasteurella multocida Pm70 Q9CMQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.194 Ni-NTA column chromatography Pasteurella multocida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.194 [protein]-Npi-phospho-L-histidine + L-ascorbate[side 1]
-
 Pasteurella multocida [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?
2.7.1.194 [protein]-Npi-phospho-L-histidine + L-ascorbate[side 1]
-
 Pasteurella multocida Pm70 [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.194 EIICasc
-
 Pasteurella multocida
2.7.1.194 L-ascorbate transporter
-
 Pasteurella multocida
2.7.1.194 UlaA
-
 Pasteurella multocida

General Information

EC Number General Information Comment Organism
2.7.1.194 metabolism the phosphotransferase system PTSasc is composed of two soluble energy-coupling proteins (EI and HPr) and an enzyme II complex (EIIA, EIIB, and EIIC) for the anaerobic uptake of ascorbate and its phosphorylation to L-ascorbate 6-phosphate in vivo Pasteurella multocida