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Literature summary extracted from

  • Kutner, J.; Shabalin, I.G.; Matelska, D.; Handing, K.B.; Gasiorowska, O.; Sroka, P.; Gorna, M.W.; Ginalski, K.; Wozniak, K.; Minor, W.
    Structural, biochemical, and evolutionary characterizations of glyoxylate/hydroxypyruvate reductases show their division into two distinct subfamilies (2018), Biochemistry, 57, 963-977 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.79 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.79 enzyme with NADP+ plus sulfate, sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M ammonium sulfate. Enzyme with NADPH plus oxalate, sitting drop vapor diffusion method, using 0.2 M ammonium citrate tribasic pH 7.0, 20% (w/v) PEG 3350. Apo enzyme form, sitting drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.6, 20% (v/v) 2-propanol, 20% (w/v) PEG 4000 Sinorhizobium meliloti
1.1.1.81 structures in apo forms and in complexes with cofactors and substrates/substrate analogues Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 9.7
-
 pyruvate at pH 7.5 and 25°C Sinorhizobium meliloti
1.1.1.81 0.0105
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 0.059
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 5.59
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 6.14
-
 3-hydroxypyruvate K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 6.58
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 9.35
-
 3-hydroxypyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 9.69
-
 pyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 11
-
 2-dehydro-D-gluconate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 12.3
-
 glyoxylate K0.5 value, Hill coefficient 1.83, pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 80
-
 glyoxylate K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NADPH + H+ Sinorhizobium meliloti enzyme GhrA shows highest catalytic efficiency for glyoxylate glycolate + NADP+
-
 ?
1.1.1.79 hydroxypyruvate + NADH + H+ Sinorhizobium meliloti with hydroxypyruvate as a substrate at a saturating concentration (66.7 mM), the enzyme GhrA exhibits 2-3% activity with 0.4 mM NADH as compared to 0.4 mM NADPH D-glycerate + NAD+
-
 ?
1.1.1.79 hydroxypyruvate + NADPH + H+ Sinorhizobium meliloti
-
 D-glycerate + NADP+
-
 ?
1.1.1.79 additional information Sinorhizobium meliloti no activity with 2-oxo-D-gluconate ?
-
-
1.1.1.79 oxalate + NADPH + H+ Sinorhizobium meliloti
-
 ?
-
 ?
1.1.1.79 pyruvate + NADPH + H+ Sinorhizobium meliloti low efficiency ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Sinorhizobium meliloti
-
-
-
1.1.1.81 Sinorhizobium meliloti Q92LZ4
-
-
1.1.1.81 Sinorhizobium meliloti Q92T34
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 Ni-NTA resin column chromatography and Superdex 200 pg gel filtration Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NADPH + H+ enzyme GhrA shows highest catalytic efficiency for glyoxylate Sinorhizobium meliloti glycolate + NADP+
-
 ?
1.1.1.79 hydroxypyruvate + NADH + H+ with hydroxypyruvate as a substrate at a saturating concentration (66.7 mM), the enzyme GhrA exhibits 2-3% activity with 0.4 mM NADH as compared to 0.4 mM NADPH Sinorhizobium meliloti D-glycerate + NAD+
-
 ?
1.1.1.79 hydroxypyruvate + NADPH + H+
-
 Sinorhizobium meliloti D-glycerate + NADP+
-
 ?
1.1.1.79 additional information no activity with 2-oxo-D-gluconate Sinorhizobium meliloti ?
-
-
1.1.1.79 oxalate + NADPH + H+
-
 Sinorhizobium meliloti ?
-
 ?
1.1.1.79 pyruvate + NADPH + H+ low efficiency Sinorhizobium meliloti ?
-
 ?
1.1.1.81 2-dehydro-D-gluconate + NADPH + H+
-
 Sinorhizobium meliloti D-gluconate + NADP+
-
 ?
1.1.1.81 3-hydroxypyruvate + NADPH + H+
-
 Sinorhizobium meliloti D-glycerate + NADP+
-
 ?
1.1.1.81 glyoxylate + NADPH + H+
-
 Sinorhizobium meliloti glycolate + NADP+
-
 ?
1.1.1.81 phenylpyruvate + NADPH + H+
-
 Sinorhizobium meliloti phenyllactate + NADP+
-
 ?
1.1.1.81 pyruvate + NADPH + H+
-
 Sinorhizobium meliloti lactate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.79 homodimer x-ray crystallography Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
1.1.1.79 GhrA
-
 Sinorhizobium meliloti
1.1.1.81 GhrA
-
 Sinorhizobium meliloti
1.1.1.81 GhrB
-
 Sinorhizobium meliloti
1.1.1.81 SMc02828
-
 Sinorhizobium meliloti
1.1.1.81 SMc04462
-
 Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NADPH
-
 Sinorhizobium meliloti
1.1.1.81 NADPH
-
 Sinorhizobium meliloti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.81 0.057
-
 pyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 0.27
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 0.37
-
 phenylpyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 0.81
-
 glyoxylate K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 0.94
-
 2-dehydro-D-gluconate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 1.2
-
 3-hydroxypyruvate pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 1.72
-
 glyoxylate Hill coefficient 1.83, pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 9.31
-
 3-hydroxypyruvate K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 371
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti
1.1.1.81 773
-
 NADPH pH 7.5, 25°C Sinorhizobium meliloti