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Literature summary extracted from

  • Hayashi, S.; Kuramata, M.; Abe, T.; Takagi, H.; Ozawa, K.; Ishikawa, S.
    Phytochelatin synthase OsPCS1 plays a crucial role in reducing arsenic levels in rice grains (2017), Plant J., 91, 840-848 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.15 As5+
-
 Oryza sativa Japonica Group
2.3.2.15 Cd2+
-
 Oryza sativa Japonica Group
2.3.2.15 additional information OsPCS1 is more sensitive to activation by As than by cadmium, whereas OsPCS2 is more weakly activated by As than by cadmium Oryza sativa Japonica Group

Application

EC Number Application Comment Organism
2.3.2.15 agriculture reduction of the level of arsenic (As) in rice grains is an important challenge for agriculture. Phytochelatin synthase OsPCS1 plays a crucial role in reducing arsenic levels in rice grains. Transgenic plants highly expressing OsPCS1 show significantly lower As levels in grains compared to wild-type plants. PCS1 is a good candidate tool to reduce As levels in rice grains Oryza sativa Japonica Group

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 gene OsPCS1, recombinant expression in Escherichia coli, recombinant expression of MBP-tagged OsPCS1 in the has2 mutant under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the nodes of the OsPCS1-overexpressing line show higher As levels than those of the wild-type Oryza sativa Japonica Group
2.3.2.15 gene OsPCS2, recombinant expression in Escherichia coli, recombinant expression of MBP-tagged OsPCS1 in the has2 mutant under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Overexpression of OsPCS2 does not fully complement the has2 mutation, although the As levels in grains became lower than in the non-transgenic has2 mutant Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.15 additional information isolation of two rice mutants (has1 and has2) in which As levels are much higher in grains but significantly lower in node I compared with the wild-type. Map-based cloning identifies the genes responsible as OsABCC1 in has1 and OsPCS1 in has2. F2 progeny obtained by crossing the mutant lines with the indica rice cultivar Habataki are genotyped by using polymorphism between cvs. Koshihikari and Habataki and measurements of As levels in grains from the F2 plants. The frequency of the high-As phenotype in F2 progeny is below 50%, suggesting that has1 and has2 are recessive mutants. Genetic linkage analyses indicate that the high-As mutant phenotypes are tightly linked to single regions on chromosome 4 in has1 and on chromosome 5 in has2 Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.15 As5+
-
 Oryza sativa Japonica Group
2.3.2.15 Cd2+
-
 Oryza sativa Japonica Group
2.3.2.15 additional information OsPCS1 is more sensitive to activation by As than by cadmium, whereas OsPCS2 is more weakly activated by As than by cadmium Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Oryza sativa Japonica Group
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Oryza sativa Japonica Group A0A1Q2SVY7 cv. Koshihikari
-
2.3.2.15 Oryza sativa Japonica Group Q0DI55 cv. Koshihikari
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.15 seed
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Oryza sativa Japonica Group Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 OsPCS1
-
 Oryza sativa Japonica Group
2.3.2.15 OsPCS2
-
 Oryza sativa Japonica Group
2.3.2.15 phytochelatin synthase
-
 Oryza sativa Japonica Group
2.3.2.15 pPhytochelatin synthase
-
 Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.15 35
-
 assay at Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.15 8
-
 assay at Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
2.3.2.15 malfunction transgenic plants highly expressing OsPCS1 show significantly lower As levels in grains than do wild-type plants Oryza sativa Japonica Group
2.3.2.15 physiological function phytochelatin synthase OsPCS1 plays a crucial role in reducing arsenic levels in rice grains. Redundancy between OsPCS1 and OsPCS2 Oryza sativa Japonica Group
2.3.2.15 physiological function redundancy between OsPCS1 and OsPCS2 Oryza sativa Japonica Group