Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yano, R.; Takagi, K.; Tochigi, S.; Fujisawa, Y.; Nomura, Y.; Tsuchinaga, H.; Takahashi, Y.; Takada, Y.; Kaga, A.; Anai, T.; Tsukamoto, C.; Seki, H.; Muranaka, T.; Ishimoto, M.
    Isolation and characterization of the soybean Sg-3 gene that is involved in genetic variation in sugar chain composition at the C-3 position in soyasaponins (2018), Plant Cell Physiol., 59, 792-805 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B82 expression in Escherichia coli Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B82 W270* induction of a premature stop codon mutation results in the absence of a terminal Glc residue on the C-3 sugar chain of soyasaponins Glycine max
2.4.1.273 R339H mutant fails to accumulate soyasaponins Ac and betag Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B82 additional information Glycine max enzymatic reaction of UGT91H9 is more efficient for soyasaponin Bb' than for Bc'. No donors: UDP-galactose or UDP-glururonate. No acceptors: soyasapogenol B 3-O-monoglucuronide and soyasapogenol B ?
-
-
2.4.1.B82 UDP-beta-D-glucose + soyasaponin Bb' Glycine max
-
 UDP + soyasaponin I
-
 ?
2.4.1.B82 UDP-beta-D-glucose + soyasaponin Bc' Glycine max
-
 UDP + soyasaponin Bx
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B82 Glycine max A0A0R0HRL5
-
-
2.4.1.273 Glycine max A0A0R0INR5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B82 additional information enzymatic reaction of UGT91H9 is more efficient for soyasaponin Bb' than for Bc'. No donors: UDP-galactose or UDP-glururonate. No acceptors: soyasapogenol B 3-O-monoglucuronide and soyasapogenol B Glycine max ?
-
-
2.4.1.B82 UDP-beta-D-glucose + soyasaponin Bb'
-
 Glycine max UDP + soyasaponin I
-
 ?
2.4.1.B82 UDP-beta-D-glucose + soyasaponin Bc'
-
 Glycine max UDP + soyasaponin Bx
-
 ?
2.4.1.273 UDP-beta-L-rhamnose + soyasaponin III
-
 Glycine max UDP + soyasaponin I
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B82 10G104700
-
 Glycine max
2.4.1.B82 UGT91H9
-
 Glycine max
2.4.1.273 SGT3
-
 Glycine max
2.4.1.273 UGT91H4
-
 Glycine max

General Information

EC Number General Information Comment Organism
2.4.1.B82 physiological function enzyme is responsible for conjugating the terminal (third) glucose at the C-3 sugar chain of soyasaponins. A double mutant lacking UGT91H9 and UGT91H4 fails to accumulate soyasaponins Ac and betag, and also soyasaponins Ab and alphag, i.e. the third Glc and Rha conjugations are both disabled Glycine max
2.4.1.273 physiological function a double mutant lacking UGT91H9 and UGT91H4 fails to accumulate soyasaponins Ac and betag, and also soyasaponins Ab and alphag, i.e. the third Glc and Rha conjugations are both disabled Glycine max