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Literature summary extracted from

  • Okitsu, N.; Matsui, K.; Horikawa, M.; Sugahara, K.; Tanaka, Y.
    Identification and characterization of novel nemophila menziesii flavone glucosyltransferases that catalyze biosynthesis of flavone 7,4'-O -diglucoside, a key component of blue metalloanthocyanins (2018), Plant Cell Physiol., 59, 2075-2085 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.B84 0.0023
-
 apigenin pH 7.5, 30°C Nemophila menziesii
2.4.1.B85 0.0067
-
 apigenin 4'-O-beta-D-glucoside pH 7.5, 30°C Nemophila menziesii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B84 Nemophila menziesii A0A292GFI2
-
-
2.4.1.B85 Nemophila menziesii A0A292GEP7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.B84 flower
-
 Nemophila menziesii
-
2.4.1.B84 leaf poor expression Nemophila menziesii
-
2.4.1.B84 petal highly expressed during the early stages of petal development Nemophila menziesii
-
2.4.1.B85 flower
-
 Nemophila menziesii
-
2.4.1.B85 leaf poor expression Nemophila menziesii
-
2.4.1.B85 petal highly expressed during the early stages of petal development Nemophila menziesii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B84 additional information no substrates: apigenin 4'-O-glucoside, luteolin 4'-O-glucoside, apigenin 7-O-glucoside or luteolin 7-O-glucoside, genistein, daidzein or betanidin Nemophila menziesii ?
-
-
2.4.1.B84 UDP-alpha-D-glucose + kaempferol 14.5% of the activity with apigenin Nemophila menziesii UDP + kaempferol 4'-O-glucoside
-
 ?
2.4.1.B84 UDP-alpha-D-glucose + luteolin 52.6% of the activity with apigenin Nemophila menziesii UDP + luteolin 4'-O-glucoside
-
 ?
2.4.1.B84 UDP-alpha-D-glucose + quercetin 62.4% of the activity with apigenin Nemophila menziesii UDP + quercetin 4'-O-glucoside
-
 ?
2.4.1.B84 UDP-D-glucose + apigenin
-
 Nemophila menziesii UDP + apigenin 4'-O-glucoside
-
 ?
2.4.1.B85 additional information F4'G7GT specifically catalyzes 7-glucosylation of flavone 4'-O-beta-D-glucosides and flavones. No substrates: genistein, daidzein or betanidin. During biosynthesis, apigenin is glucosylated first at the 4'-O-position by 4'-O-glucosyltransferase, and then at the 7-O-position Nemophila menziesii ?
-
-
2.4.1.B85 UDP-alpha-D-glucose + apigenin reaction of EC 2.4.1.81, 27.8% of the activity with apigenin 4'-O-beta-D-glucoside Nemophila menziesii UDP + apigenin 7-O-beta-D-glucoside
-
 ?
2.4.1.B85 UDP-alpha-D-glucose + apigenin 4'-O-beta-D-glucoside
-
 Nemophila menziesii UDP + apigenin 4',7-O-beta-D-diglucoside
-
 ?
2.4.1.B85 UDP-alpha-D-glucose + luteolin
-
 Nemophila menziesii UDP + luteolin 7-O-beta-D-glucoside
-
 ?
2.4.1.B85 UDP-alpha-D-glucose + luteolin 4'-O-beta-D-glucoside 42.4% of the activity with apigenin 4'-O-beta-D-glucoside Nemophila menziesii UDP + luteolin 4',7-O-beta-D-diglucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B84 F4'GT
-
 Nemophila menziesii
2.4.1.B85 F4'G7GT
-
 Nemophila menziesii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B84 30
-
 assay at Nemophila menziesii
2.4.1.B85 30
-
 assay at Nemophila menziesii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.B84 0.22
-
 apigenin pH 7.5, 30°C Nemophila menziesii
2.4.1.B85 0.73
-
 apigenin 4'-O-beta-D-glucoside pH 7.5, 30°C Nemophila menziesii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B84 7.5
-
 assay at Nemophila menziesii
2.4.1.B85 7.5
-
 assay at Nemophila menziesii

General Information

EC Number General Information Comment Organism
2.4.1.B84 physiological function Nicotiana tabacum cells expressing both UDP-glucose:flavone 4'-O-glycosyltransferase and F4'G7GT synthesize apigenin 7,4'-O-diglucoside in presence of apigenin Nemophila menziesii
2.4.1.B85 physiological function Nicotiana tabacum cells expressing both UDP-glucose:flavone 4'-O-glycosyltransferase and F4'G7GT synthesize apigenin 7,4'-O-beta-D-diglucoside in presence of apigenin Nemophila menziesii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.B84 10
-
 apigenin pH 7.5, 30°C Nemophila menziesii
2.4.1.B85 10.5
-
 apigenin 4'-O-beta-D-glucoside pH 7.5, 30°C Nemophila menziesii