Literature summary extracted from
Zocher, G.; Winkler, R.; Hertweck, C.; Schulz, G.
Structure and action of the N-oxygenase AurF from Streptomyces thioluteus (2007), J. Mol. Biol., 373, 65-74 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.99.68 |
expression in Escherichia coli as a fusion with an N-terminal maltose-binding protein |
Streptomyces thioluteus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.14.99.68 |
structure at 2.1 A resolution shows a homodimer with a binuclear manganese cluster and an iron content of about 15%. An additional histidine residue in the coordination sphere causes the preference for manganese over iron. There is no oxo-bridge |
Streptomyces thioluteus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.99.68 |
F264A |
105% of wild-type activity |
Streptomyces thioluteus |
1.14.99.68 |
L202F |
357% of wild-type activity |
Streptomyces thioluteus |
1.14.99.68 |
L300W |
80% of wild-type activity |
Streptomyces thioluteus |
1.14.99.68 |
R96A |
complete loss of activity |
Streptomyces thioluteus |
1.14.99.68 |
T100A |
314% of wild-type activity |
Streptomyces thioluteus |
1.14.99.68 |
T100L |
14% of wild-type activity |
Streptomyces thioluteus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.99.68 |
Iron |
iron content is about 15% |
Streptomyces thioluteus |
|
1.14.99.68 |
Manganese |
crystal structure shows a binuclear manganese cluster |
Streptomyces thioluteus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.99.68 |
Streptomyces thioluteus |
Q70KH9 |
- |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.99.68 |
0.0032 |
- |
23°C, pH 7.5 |
Streptomyces thioluteus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.99.68 |
4-aminobenzoate + reduced acceptor + 2 O2 |
- |
Streptomyces thioluteus |
4-nitrobenzoate + acceptor + 2 H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.99.68 |
dimer |
crystal structure shows a homodimer |
Streptomyces thioluteus |