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Literature summary extracted from

  • Jain, P.; Sethi, S.C.; Pratyusha, V.A.; Garai, P.; Naqvi, N.; Singh, S.; Pawar, K.; Puri, N.; Komath, S.S.
    Ras signaling activates glycosylphosphatidylinositol (GPI) anchor biosynthesis via the GPI-N-acetylglucosaminyltransferase (GPI-GnT) in Candida albicans (2018), J. Biol. Chem., 293, 12222-12238 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.198 CaRas1 localizes to the cytoplasm or endoplasmic reticulum (ER) is sufficient for GPI-GnT activation. Mutational analysis, overview Candida albicans
2.4.1.198 additional information Ras signaling enhances GPI-GnT activity Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.198 gene GPI1, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis Candida albicans
2.4.1.198 gene GPI15, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, phenotype, RT-PCR enzyme expression analysis Candida albicans
2.4.1.198 gene GPI19, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis Candida albicans
2.4.1.198 gene GPI2, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, phenotype, RT-PCR enzyme expression analysis Candida albicans
2.4.1.198 gene GPI3, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.198 additional information analysis of enzyme-deficient WY-ZY4 strain (BWP17- Caras1/Caras2 mutant). CaGpi2 overexpression results in Hsp90 down-regulation, which is reflected in a decrease in transcript levels as well as in Hsp90 activity. Depletion of CaGpi2 can perhaps reduce the levels of CaRas1 available at the PM for signaling in the cAMP/PKA pathway for hyphal morphogenesis. Generation of revertant strains for all GPI-GnT subunits are created in their respective heterozygous strains Candida albicans
2.4.1.198 additional information analysis of enzyme-deficient WY-ZY4 strain (BWP17- Caras1/Caras2 mutant). Depletion of CaGpi2 can perhaps reduce the levels of CaRas1 available at the PM for signaling in the cAMP/PKA pathway for hyphal morphogenesis. Generation of revertant strains for all GPI-GnT subunits are created in their respective heterozygous strains Candida albicans
2.4.1.198 additional information analysis of enzyme-deficient WY-ZY4 strain (BWP17- Caras1/Caras2 mutant). Generation of revertant strains for all GPI-GnT subunits are created in their respective heterozygous strains Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.198 microsome
-
 Candida albicans
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.198 UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol Candida albicans
-
 UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.198 UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol Candida albicans ATCC MYA-2876
-
 UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.198 Candida albicans
-
-
-
2.4.1.198 Candida albicans A0A1D8PHD4
-
-
2.4.1.198 Candida albicans A0A1D8PKB8
-
-
2.4.1.198 Candida albicans Q5A6L6
-
-
2.4.1.198 Candida albicans Q5A9D6
-
-
2.4.1.198 Candida albicans ATCC MYA-2876
-
-
-
2.4.1.198 Candida albicans ATCC MYA-2876 A0A1D8PHD4
-
-
2.4.1.198 Candida albicans ATCC MYA-2876 A0A1D8PKB8
-
-
2.4.1.198 Candida albicans ATCC MYA-2876 Q5A6L6
-
-
2.4.1.198 Candida albicans ATCC MYA-2876 Q5A9D6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.198 UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
-
 Candida albicans UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.198 UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
-
 Candida albicans ATCC MYA-2876 UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.198 GPI-GnT
-
 Candida albicans
2.4.1.198 GPI-N-acetylglucosaminyltransferase
-
 Candida albicans
2.4.1.198 GPI1
-
 Candida albicans
2.4.1.198 GPI15
-
 Candida albicans
2.4.1.198 GPI19
-
 Candida albicans
2.4.1.198 GPI2
-
 Candida albicans
2.4.1.198 GPI3
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.198 30
-
 assay at Candida albicans

General Information

EC Number General Information Comment Organism
2.4.1.198 malfunction in the CaGPI19 conditional null strain, isozyme CaGpi2 is overexpressed Candida albicans
2.4.1.198 malfunction the strain overexpressing CaGpi2 is hyperfilamentous and also heat-shock-sensitive, a phenotype typical of hyperactive Ras mutants. When Hsp90 levels are downregulated, due to CaGpi2 overexpression, the interaction of CaRas1 with Cyr1 is promoted at the cost of its interaction with Ira2. Thus, the filamentation pathway remains turned on even at 30°C, resulting in a hyperfilamentous phenotype Candida albicans
2.4.1.198 metabolism the ability of Candida albicans to switch between yeast to hyphal form is a property that is primarily associated with the invasion and virulence of this human pathogenic fungus. Several glycosylphosphatidylinositol (GPI)-anchored proteins are expressed only during hyphal morphogenesis. One of the major pathways that controls hyphal morphogenesis is the Ras signaling pathway. Cross-talk between GPI anchor biosynthesis and Ras signaling occurs in Candida albicans. The first step of GPI biosynthesis is activated by Ras in Candida albicans. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT Candida albicans
2.4.1.198 metabolism the ability of Candida albicans to switch between yeast to hyphal form is a property that is primarily associated with the invasion and virulence of this human pathogenic fungus. Several glycosylphosphatidylinositol (GPI)-anchored proteins are expressed only during hyphal morphogenesis. One of the major pathways that controls hyphal morphogenesis is the Ras signaling pathway. Cross-talk between GPI anchor biosynthesis and Ras signaling occurs in Candida albicans. The first step of GPI biosynthesis is activated by Ras in Candida albicans. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT. Possible interaction between Ras signaling and GPI-GnT in Candida albicans, detailed overview Candida albicans
2.4.1.198 physiological function of the two Candida albicans Ras proteins, CaRas1 alone activates GPI-GnT activity, and the activity is further stimulated by constitutively activated CaRas1. Of the six subunits of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) that catalyze the first step of GPI biosynthesis, CaGpi2 is the key player involved in activating Ras signaling and hyphal morphogenesis. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT Candida albicans