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Literature summary extracted from

  • Knejzlik, Z.; Herkommerova, K.; Hockova, D.; Pichova, I.
    Hypoxanthine-guanine phosphoribosyltransferase is dispensable for Mycobacterium smegmatis viability (2020), J. Bacteriol., 202, e00710-19 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.2.8 drug development the hypoxanthineguanine phosphoribosyltransferase (HGPRT) represents a potential target for specific inhibitor development Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 additional information the antimicrobial activity of acyclic nucleoside phosphonates (ANP) prodrugs diethyl (2R,15S)-7-[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl]-2-benzyl-4,13-bis[[(2S)-1-ethoxy-1-oxo-3-phenylpropan-2-yl]amino]-10-(2-hydroxyethyl)-4,13-dioxo-15-(2-phenylethyl)-3,7,10,14-tetraaza-4lambda5,13lambda5-diphosphahexadecane-1,16-dioate and diethyl (2R,15S)-2-benzyl-4,13-bis[[(2S)-1-ethoxy-1-oxo-3-phenylpropan-2-yl]amino]-10-(2-hydroxyethyl)-4,13-dioxo-7-[2-(6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl]-15-(2-phenylethyl)-3,7,10,14-tetraaza-4lambda5,13lambda5-diphosphahexadecane-1,16-dioate is not attributable to inhibition of HGPRT Mycolicibacterium smegmatis
2.4.2.8 [2-([2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl][2-[(2-hydroxyethyl)(2-phosphonoethyl)amino]ethyl]amino)ethyl]phosphonic acid
-
 Mycolicibacterium smegmatis
2.4.2.8 [2-[(2-hydroxyethyl)(2-[[2-(6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl](2-phosphonoethyl)amino]ethyl)amino]ethyl]phosphonic acid
-
 Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 guanosine + 5-phospho-alpha-D-ribose 1-diphosphate Mycolicibacterium smegmatis
-
 GMP + diphosphate
-
 ?
2.4.2.8 guanosine + 5-phospho-alpha-D-ribose 1-diphosphate Mycolicibacterium smegmatis ATCC 700084
-
 GMP + diphosphate
-
 ?
2.4.2.8 guanosine + 5-phospho-alpha-D-ribose 1-diphosphate Mycolicibacterium smegmatis mc(2)155
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Mycolicibacterium smegmatis
-
 IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Mycolicibacterium smegmatis ATCC 700084
-
 IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Mycolicibacterium smegmatis mc(2)155
-
 IMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Mycolicibacterium smegmatis A0R593
-
-
2.4.2.8 Mycolicibacterium smegmatis ATCC 700084 A0R593
-
-
2.4.2.8 Mycolicibacterium smegmatis mc(2)155 A0R593
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanosine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis GMP + diphosphate
-
 ?
2.4.2.8 guanosine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis ATCC 700084 GMP + diphosphate
-
 ?
2.4.2.8 guanosine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis mc(2)155 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis ATCC 700084 IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycolicibacterium smegmatis mc(2)155 IMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGPRT
-
 Mycolicibacterium smegmatis
2.4.2.8 HPT
-
 Mycolicibacterium smegmatis
2.4.2.8 hypoxanthine-guanine phosphoribosyltransferase
-
 Mycolicibacterium smegmatis
2.4.2.8 MSMEG_6110 locus name Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 37
-
 assay at Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
2.4.2.8 malfunction deletion of the HGPRT gene (DELTAhgprt) in the model organism Mycobacterium smegmatis confirms that this enzyme is not essential for Mycobacterium smegmatis' growth Mycolicibacterium smegmatis
2.4.2.8 physiological function hypoxanthine-guanine phosphoribosyltransferase (HGPRT) catalyzes the formation of guanosine-5'-monophosphate from guanine and inosine-5'-monophosphate from hypoxanthine. It is the key enzyme in the purine salvage pathway, catalyzes the synthesis of inosine- or guanosine-5'-monophosphate via replacement of the 1-diphosphate group in phosphoribosyl diphosphate with a corresponding free nucleobase. Deletion of the HGPRT gene (DELTAhgprt) in the model organism Mycobacterium smegmatis confirms that this enzyme is not essential for Mycobacterium smegmatis' growth Mycolicibacterium smegmatis