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Literature summary extracted from

  • Dindo, M.; Costanzi, E.; Pieroni, M.; Costantini, C.; Annunziato, G.; Bruno, A.; Keller, N.P.; Romani, L.; Zelante, T.; Cellini, B.
    Biochemical characterization of Aspergillus fumigatus AroH, a putative aromatic amino acid aminotransferase (2018), Front. Mol. Biosci., 5, 104 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.57 recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.14
-
 alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate Aspergillus fumigatus
2.6.1.57 0.18
-
 2-oxoglutarate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan Aspergillus fumigatus
2.6.1.57 0.5
-
 L-tyrosine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 1.4
-
 L-phenylalanine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 2.1
-
 L-tryptophan pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 3.3
-
 alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 9
-
 L-kynurenine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 10
-
 L-glutamate pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 107000
-
 recombinant enzyme, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.57 an aromatic amino acid + 2-oxoglutarate Aspergillus fumigatus
-
 an aromatic oxo acid + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 2-oxoglutarate + L-phenylalanine
-
 Aspergillus fumigatus L-glutamate + phenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-tryptophan
-
 Aspergillus fumigatus L-glutamate + 3-indole-2-oxopropanoate
-
 r
2.6.1.57 2-oxoglutarate + L-tyrosine
-
 Aspergillus fumigatus L-glutamate + 4-hydroxyphenylpyruvate
-
 r
2.6.1.57 alpha-aminoadipate + 2-oxoglutarate
-
 Aspergillus fumigatus 2-oxoadipate + L-glutamate
-
 r
2.6.1.57 alpha-aminoadipate + L-glutamate
-
 Aspergillus fumigatus 2-oxoglutarate + ?
-
 r
2.6.1.57 an aromatic amino acid + 2-oxoglutarate
-
 Aspergillus fumigatus an aromatic oxo acid + L-glutamate
-
 r
2.6.1.57 L-kynurenine + 2-oxoglutarate reaction of EC 2.6.1.7 Aspergillus fumigatus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
-
 r
2.6.1.57 additional information AroH behaves as an aromatic amino acid aminotransferase, but also accepts L-kynurenine and alpha-aminoadipate as amino donors Aspergillus fumigatus ?
-
-

Subunits

EC Number Subunits Comment Organism
2.6.1.57 homodimer 2 * 59480, sequence calculation, 2 * 57000, recombinant enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.57 AroH
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.57 25
-
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.57 2.5
-
 alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate Aspergillus fumigatus
2.6.1.57 3
-
 L-tryptophan pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 4
-
 L-tyrosine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 4.1
-
 L-kynurenine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 7.4
-
 L-phenylalanine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 10.3
-
 L-glutamate pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate Aspergillus fumigatus
2.6.1.57 12.5
-
 2-oxoglutarate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan Aspergillus fumigatus
2.6.1.57 13.2
-
 alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.57 7.4
-
-
 Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate PLP, the enzyme binds the coenzyme forming a deprotonated internal aldimine in equilibrium with a protonated ketoenamine form Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.6.1.57 additional information homology modeling of the AroH structure using the structure of Saccharomyces cerevisiae Aro8 (PDB ID 4JE5) as template Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.57 0.4
-
 L-kynurenine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 0.96
-
 L-glutamate pH 7.4, 25°C, recombinant enzyme, with cosubstrate alpha-aminoadipate Aspergillus fumigatus
2.6.1.57 1.4
-
 L-tryptophan pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 4
-
 alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 5.2
-
 L-phenylalanine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 8
-
 L-tyrosine pH 7.4, 25°C, recombinant enzyme, with cosubstrate 2-oxoglutarate Aspergillus fumigatus
2.6.1.57 14
-
 alpha-aminoadipate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-glutamate Aspergillus fumigatus
2.6.1.57 90
-
 2-oxoglutarate pH 7.4, 25°C, recombinant enzyme, with cosubstrate L-tryptophan Aspergillus fumigatus