EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.183 | gene agsA, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans | Aspergillus nidulans |
2.4.1.183 | gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans | Aspergillus nidulans |
2.4.1.183 | gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans | Aspergillus oryzae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.183 | additional information | overexpression of agsA in Aspergillus nidulans under the control of a constitutive promoter in the genetic background of agsB disruptants, the native promoter is replaced by the constitutive tef1 promoter. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan. The agsA gene is disrupted by using the Cre/loxP marker recycling system. Generation of an agsA deletion mutant strain agsADELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan | Aspergillus nidulans |
2.4.1.183 | additional information | overexpression of agsB in Aspergillus nidulans under control of a constitutive promoter in the genetic background of agsA disruptants, the native promoter is replaced by the constitutive tef1 promoter. Generation of an agsB deletion mutant strain agsBDELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan | Aspergillus nidulans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.183 | cell wall | - |
Aspergillus nidulans | 5618 | - |
2.4.1.183 | cell wall | - |
Aspergillus oryzae | 5618 | - |
2.4.1.183 | intracellular | - |
Aspergillus nidulans | 5622 | - |
2.4.1.183 | additional information | alpha-1,3-glucan is located in the inner layer in the agsA overexpressing strain | Aspergillus nidulans | - |
- |
2.4.1.183 | additional information | alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain | Aspergillus nidulans | - |
- |
2.4.1.183 | additional information | alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain | Aspergillus oryzae | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.183 | UDP-glucose + [alpha-D-glucosyl-(1->3)]n | Aspergillus nidulans | - |
UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
2.4.1.183 | UDP-glucose + [alpha-D-glucosyl-(1->3)]n | Aspergillus oryzae | - |
UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
2.4.1.183 | UDP-glucose + [alpha-D-glucosyl-(1->3)]n | Aspergillus nidulans ABPU1 | - |
UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.183 | Aspergillus nidulans | - |
- |
- |
2.4.1.183 | Aspergillus nidulans ABPU1 | - |
- |
- |
2.4.1.183 | Aspergillus oryzae | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.183 | huelle cell | nursing cells of cleistothecia | Aspergillus nidulans | - |
2.4.1.183 | hypha | - |
Aspergillus nidulans | - |
2.4.1.183 | hypha | - |
Aspergillus oryzae | - |
2.4.1.183 | mycelium | - |
Aspergillus nidulans | - |
2.4.1.183 | mycelium | - |
Aspergillus oryzae | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.183 | UDP-glucose + [alpha-D-glucosyl-(1->3)]n | - |
Aspergillus nidulans | UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
2.4.1.183 | UDP-glucose + [alpha-D-glucosyl-(1->3)]n | - |
Aspergillus oryzae | UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
2.4.1.183 | UDP-glucose + [alpha-D-glucosyl-(1->3)]n | - |
Aspergillus nidulans ABPU1 | UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.183 | agsA | - |
Aspergillus nidulans |
2.4.1.183 | agsB | - |
Aspergillus nidulans |
2.4.1.183 | agsB | - |
Aspergillus oryzae |
2.4.1.183 | alpha-1,3-glucan synthase | - |
Aspergillus nidulans |
2.4.1.183 | alpha-1,3-glucan synthase | - |
Aspergillus oryzae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.183 | malfunction | the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan | Aspergillus nidulans |
2.4.1.183 | malfunction | the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan | Aspergillus nidulans |
2.4.1.183 | malfunction | the disruption of the agsB gene leads to the loss of alpha-1,3-glucan. Overexpression of agsB increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan. The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains | Aspergillus oryzae |
2.4.1.183 | additional information | determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the inner layer in the agsA overexpressing strain | Aspergillus nidulans |
2.4.1.183 | additional information | determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain | Aspergillus nidulans |
2.4.1.183 | additional information | determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain | Aspergillus oryzae |
2.4.1.183 | physiological function | alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular | Aspergillus nidulans |
2.4.1.183 | physiological function | alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions | Aspergillus nidulans |
2.4.1.183 | physiological function | alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions | Aspergillus oryzae |