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Literature summary extracted from

  • Andreessen, C.; Gerlt, V.; Steinbuechel, A.
    Conversion of cysteine to 3-mercaptopyruvic acid by bacterial aminotransferases (2017), Enzyme Microb. Technol., 99, 38-48 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.1 expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator
2.6.1.1 expressed in Escherichia coli BL21(DE3) cells Shimwellia blattae
2.6.1.1 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
2.6.1.1 expressed in Escherichia coli BL21(DE3) cells Advenella mimigardefordensis
2.6.1.57 expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator
2.6.1.57 expressed in Escherichia coli BL21(DE3) cells Shimwellia blattae
2.6.1.57 expressed in Escherichia coli BL21(DE3) cells Paracoccus denitrificans
2.6.1.57 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.039
-
 L-aspartate at pH 7.4 and 30°C Advenella mimigardefordensis
2.6.1.1 0.108
-
 L-aspartate at pH 7.4 and 30°C Escherichia coli
2.6.1.1 8.57
-
 L-cysteine at pH 7.4 and 30°C Advenella mimigardefordensis
2.6.1.1 20.62
-
 L-cysteine at pH 7.4 and 30°C Escherichia coli
2.6.1.57 0.079
-
 L-aspartate at pH 7.4 and 30°C Paracoccus denitrificans
2.6.1.57 12.22
-
 L-cysteine at pH 7.4 and 30°C Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Cupriavidus necator
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Shimwellia blattae
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Escherichia coli
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Advenella mimigardefordensis
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Advenella mimigardefordensis DPN7
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Escherichia coli MG1655
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate Shimwellia blattae ATCC 33430
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Cupriavidus necator
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Shimwellia blattae
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Paracoccus denitrificans
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Escherichia coli
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Escherichia coli MG1655
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Shimwellia blattae ATCC 33430
-
 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate Paracoccus denitrificans Pd1222
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Advenella mimigardefordensis W0PFQ7
-
-
2.6.1.1 Advenella mimigardefordensis DPN7 W0PFQ7
-
-
2.6.1.1 Cupriavidus necator
-
-
-
2.6.1.1 Escherichia coli P00509
-
-
2.6.1.1 Escherichia coli MG1655 P00509
-
-
2.6.1.1 Shimwellia blattae
-
-
-
2.6.1.1 Shimwellia blattae ATCC 33430
-
-
-
2.6.1.57 Cupriavidus necator
-
-
-
2.6.1.57 Escherichia coli P04693
-
-
2.6.1.57 Escherichia coli MG1655 P04693
-
-
2.6.1.57 Paracoccus denitrificans P95468
-
-
2.6.1.57 Paracoccus denitrificans Pd1222 P95468
-
-
2.6.1.57 Shimwellia blattae
-
-
-
2.6.1.57 Shimwellia blattae ATCC 33430
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 His SpinTrap column chromatography Cupriavidus necator
2.6.1.1 His SpinTrap column chromatography Shimwellia blattae
2.6.1.1 His SpinTrap column chromatography Escherichia coli
2.6.1.1 His SpinTrap column chromatography Advenella mimigardefordensis
2.6.1.57 His SpinTrap column chromatography Cupriavidus necator
2.6.1.57 His SpinTrap column chromatography Shimwellia blattae
2.6.1.57 His SpinTrap column chromatography Paracoccus denitrificans
2.6.1.57 His SpinTrap column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Cupriavidus necator oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Shimwellia blattae oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Escherichia coli oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Advenella mimigardefordensis oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Advenella mimigardefordensis DPN7 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Escherichia coli MG1655 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Shimwellia blattae ATCC 33430 oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Cupriavidus necator 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Shimwellia blattae 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Escherichia coli 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Advenella mimigardefordensis 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Advenella mimigardefordensis DPN7 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Escherichia coli MG1655 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate
-
 Shimwellia blattae ATCC 33430 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Cupriavidus necator oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Shimwellia blattae oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Paracoccus denitrificans oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Escherichia coli oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Escherichia coli MG1655 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Shimwellia blattae ATCC 33430 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Paracoccus denitrificans Pd1222 oxaloacetate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Cupriavidus necator 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Shimwellia blattae 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Paracoccus denitrificans 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Escherichia coli 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Escherichia coli MG1655 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Shimwellia blattae ATCC 33430 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.57 L-cysteine + 2-oxoglutarate
-
 Paracoccus denitrificans Pd1222 3-mercaptopyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 ? x * 40000, SDS-PAGE Cupriavidus necator
2.6.1.1 ? x * 40000, SDS-PAGE Shimwellia blattae
2.6.1.1 ? x * 40000, SDS-PAGE Escherichia coli
2.6.1.1 ? x * 40000, SDS-PAGE Advenella mimigardefordensis
2.6.1.1 ? x * 43900, calculated from amino acid sequence Advenella mimigardefordensis
2.6.1.1 ? x * 43600, calculated from amino acid sequence Escherichia coli
2.6.1.1 ? x * 43800, calculated from amino acid sequence Shimwellia blattae
2.6.1.1 ? x * 43200, calculated from amino acid sequence Cupriavidus necator
2.6.1.57 ? x * 40000, SDS-PAGE Cupriavidus necator
2.6.1.57 ? x * 40000, SDS-PAGE Shimwellia blattae
2.6.1.57 ? x * 40000, SDS-PAGE Paracoccus denitrificans
2.6.1.57 ? x * 40000, SDS-PAGE Escherichia coli
2.6.1.57 ? x * 43600, calculated from amino acid sequence Cupriavidus necator
2.6.1.57 ? x * 43100, calculated from amino acid sequence Shimwellia blattae
2.6.1.57 ? x * 42700, calculated from amino acid sequence Paracoccus denitrificans
2.6.1.57 ? x * 43500, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 AAT
-
 Cupriavidus necator
2.6.1.1 AAT
-
 Shimwellia blattae
2.6.1.1 AAT
-
 Escherichia coli
2.6.1.1 AAT
-
 Advenella mimigardefordensis
2.6.1.1 aspartate aminotransferase
-
 Cupriavidus necator
2.6.1.1 aspartate aminotransferase
-
 Shimwellia blattae
2.6.1.1 aspartate aminotransferase
-
 Escherichia coli
2.6.1.1 aspartate aminotransferase
-
 Advenella mimigardefordensis
2.6.1.1 aspC
-
 Cupriavidus necator
2.6.1.1 aspC
-
 Shimwellia blattae
2.6.1.1 aspC
-
 Escherichia coli
2.6.1.1 aspC
-
 Advenella mimigardefordensis
2.6.1.57 ArAT
-
 Cupriavidus necator
2.6.1.57 ArAT
-
 Shimwellia blattae
2.6.1.57 ArAT
-
 Paracoccus denitrificans
2.6.1.57 ArAT
-
 Escherichia coli
2.6.1.57 aromatic amino acid aminotransferase
-
 Cupriavidus necator
2.6.1.57 aromatic amino acid aminotransferase
-
 Shimwellia blattae
2.6.1.57 aromatic amino acid aminotransferase
-
 Paracoccus denitrificans
2.6.1.57 aromatic amino acid aminotransferase
-
 Escherichia coli
2.6.1.57 TyrB
-
 Cupriavidus necator
2.6.1.57 TyrB
-
 Shimwellia blattae
2.6.1.57 TyrB
-
 Paracoccus denitrificans
2.6.1.57 TyrB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 35
-
-
 Escherichia coli
2.6.1.1 40
-
-
 Advenella mimigardefordensis
2.6.1.57 40
-
-
 Paracoccus denitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.1 19.66
-
 L-aspartate at pH 7.4 and 30°C Advenella mimigardefordensis
2.6.1.1 24.43
-
 L-aspartate at pH 7.4 and 30°C Escherichia coli
2.6.1.1 40.64
-
 L-cysteine at pH 7.4 and 30°C Escherichia coli
2.6.1.1 43.85
-
 L-cysteine at pH 7.4 and 30°C Advenella mimigardefordensis
2.6.1.57 16.89
-
 L-cysteine at pH 7.4 and 30°C Paracoccus denitrificans
2.6.1.57 16.9
-
 L-aspartate at pH 7.4 and 30°C Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate
-
 Cupriavidus necator
2.6.1.1 pyridoxal 5'-phosphate
-
 Shimwellia blattae
2.6.1.1 pyridoxal 5'-phosphate
-
 Escherichia coli
2.6.1.1 pyridoxal 5'-phosphate
-
 Advenella mimigardefordensis
2.6.1.57 pyridoxal 5'-phosphate
-
 Cupriavidus necator
2.6.1.57 pyridoxal 5'-phosphate
-
 Shimwellia blattae
2.6.1.57 pyridoxal 5'-phosphate
-
 Paracoccus denitrificans
2.6.1.57 pyridoxal 5'-phosphate
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Shimwellia blattae calculated from amino acid sequence
-
 5.4
2.6.1.1 Escherichia coli calculated from amino acid sequence
-
 5.5
2.6.1.1 Advenella mimigardefordensis calculated from amino acid sequence
-
 6
2.6.1.1 Cupriavidus necator calculated from amino acid sequence
-
 6.2
2.6.1.57 Shimwellia blattae calculated from amino acid sequence
-
 5.4
2.6.1.57 Paracoccus denitrificans calculated from amino acid sequence
-
 5.5
2.6.1.57 Cupriavidus necator calculated from amino acid sequence
-
 6.3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.1 2
-
 L-cysteine at pH 7.4 and 30°C Escherichia coli
2.6.1.1 5.1
-
 L-cysteine at pH 7.4 and 30°C Advenella mimigardefordensis
2.6.1.1 226.2
-
 L-aspartate at pH 7.4 and 30°C Escherichia coli
2.6.1.1 504.1
-
 L-aspartate at pH 7.4 and 30°C Advenella mimigardefordensis
2.6.1.57 1.4
-
 L-cysteine at pH 7.4 and 30°C Paracoccus denitrificans
2.6.1.57 214
-
 L-aspartate at pH 7.4 and 30°C Paracoccus denitrificans