Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Satoh, S.; Ozaki, M.; Matsumoto, S.; Nabatame, T.; Kaku, M.; Shudo, T.; Asayama, M.; Chohnan, S.
    Enhancement of fatty acid biosynthesis by exogenous acetyl-CoA carboxylase and pantothenate kinase in Escherichia coli (2020), Biotechnol. Lett., 42, 2595-2605 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 gene coaX, functional recombinant expression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.33 additional information expression of acetyl-CoA carboxylase (acc) obtained from Corynebacterium glutamicum in Escherichia coli, it causes accumulation of 2.2fold more fatty acids in Escherichia coli that in the wild-type. The addition of gene coaX encoding patothenate kinase from Pseudomonas putida or fatty acid synthase (fasA) from Corynebacterium glutamicum results in a 3.1 and 3.6fold increased fatty acid synthesis in Escherichia coli cells, which express acc and coaA, or acc and fasA, respectively. The transformants, simultaneously possessing all three genes, produce 5.6fold more fatty acids. The strain possessing acc, coaA, and fasA stores 691 mg/l of fatty acids, primarily as phospholipids, inside the inner membrane after 72-h cultivation. In addition, 19% of the total CoA pool is occupied by malonyl-CoA Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Pseudomonas putida
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Pseudomonas putida V5XWU6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Pseudomonas putida ADP + (R)-4'-phosphopantothenate
-
 ?