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Literature summary extracted from

  • Yang, K.; Li, S.M.; Liu, X.; Fan, A.
    Reinvestigation of the substrate specificity of a reverse prenyltransferase NotF from Aspergillus sp. MF297-2 (2020), Arch. Microbiol., 202, 1419-1424 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.109 expressed in Escherichia coli BL21(DE3) cells Aspergillus sp. MF297-2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.109 dimethylallyl diphosphate + brevianamide F Aspergillus sp. MF297-2
-
 diphosphate + deoxybrevianamide E
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.109 Aspergillus sp. MF297-2 E0Y3X1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.109 Ni-NTA agarose column chromatography Aspergillus sp. MF297-2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.109 dimethylallyl diphosphate + brevianamide F
-
 Aspergillus sp. MF297-2 diphosphate + deoxybrevianamide E
-
 ?
2.5.1.109 dimethylallyl diphosphate + brevianamide F i.e. cyclo-L-Trp-L-Pro, conversion yield of 66% Aspergillus sp. MF297-2 diphosphate + deoxybrevianamide E
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Ala conversion yield of 1% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Ala conversion yield of less than 3% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Pro conversion yield of 6% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-L-Ala conversion yield of 17% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-L-Pro conversion yield of 18% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-D-Ala conversion yield of 5% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-D-Pro conversion yield of 61% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-Gly conversion yield of 6% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Ala conversion yield of 11% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-His conversion yield of less than 3% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Phe conversion yield of less than 3% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Trp conversion yield of less than 3% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr conversion yield of less than 3% Aspergillus sp. MF297-2 ?
-
 ?
2.5.1.109 additional information the enzyme shows no activity with L-Trp, cyclo-L-Trp-L-Trp, cyclo-L-Phe-L-Pro and cyclo-L-Trp-L-Tyr Aspergillus sp. MF297-2 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.109 ? x * 53000, SDS-PAGE Aspergillus sp. MF297-2

Synonyms

EC Number Synonyms Comment Organism
2.5.1.109 NotF
-
 Aspergillus sp. MF297-2

General Information

EC Number General Information Comment Organism
2.5.1.109 metabolism the enzyme is involved in the biosynthesis of notoamides Aspergillus sp. MF297-2