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Literature summary extracted from

  • Brown, B.L.; Kardon, J.R.; Sauer, R.T.; Baker, T.A.
    Structure of the mitochondrial aminolevulinic acid synthase, a key heme biosynthetic enzyme (2018), Structure, 26, 580-589.e4 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.37 in complex with pyridoxal 5'-phosphate, using 0.1 M tri-sodium citrate (pH 5.5), 20% (w/v) PEG 3000 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 DELTACT the variant lacking residues 535-548 has reduced activity (about 65% of wild type) Saccharomyces cerevisiae
2.3.1.37 R151A the mutant shows 20% of wild type activity Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.37 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine Saccharomyces cerevisiae
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Saccharomyces cerevisiae P09950
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 Ni2+-NTA agarose resin column chromatography and Superdex S200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Saccharomyces cerevisiae 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 homodimer
-
 Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.37 5-aminolevulinic acid synthase
-
 Saccharomyces cerevisiae
2.3.1.37 ALAS
-
 Saccharomyces cerevisiae
2.3.1.37 Hem1
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate one enzyme subunit contains covalently bound pyridoxal 5'-phosphate whereas the second is pyridoxal 5'-phosphate-free Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.3.1.37 metabolism the enzyme catalyzes the first step in heme biosynthesis Saccharomyces cerevisiae