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Literature summary extracted from

  • Wagner, T.; Ermler, U.; Shima, S.
    MtrA of the sodium ion pumping methyltransferase binds cobalamin in a unique mode (2016), Sci. Rep., 6, 28226 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.86 expressed in Escherichia coli BL21(DE3) cells Methanocaldococcus jannaschii
2.1.1.86 expressed in Escherichia coli BL21(DE3) cells Methanothermus fervidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.86 in complex with either hydroxocobalamin or methylcobalamin, vapor diffusion method, using 26% (w/v) PEG 3000, 100 mM Na citrate pH 5.5 Methanothermus fervidus
2.1.1.86 in complex with either hydroxocobalamin or methylcobalamin, vapor diffusion method, using 26% (w/v) PEG 3000, 2.2 M DL-malate pH 7.6, 100 mM Tris-HCl pH 8.0 Methanocaldococcus jannaschii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.86 cytoplasm
-
 Methanothermus fervidus 5737
-
2.1.1.86 membrane
-
 Methanocaldococcus jannaschii 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in Methanocaldococcus jannaschii
-
 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in Methanothermus fervidus
-
 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in Methanothermus fervidus ATCC 43054
-
 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.86 Methanocaldococcus jannaschii
-
-
-
2.1.1.86 Methanothermus fervidus E3GWY7
-
-
2.1.1.86 Methanothermus fervidus ATCC 43054 E3GWY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.86 Ni2+ -charged HiTrap column chromatography Methanocaldococcus jannaschii
2.1.1.86 Ni2+ -charged HiTrap column chromatography Methanothermus fervidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in
-
 Methanocaldococcus jannaschii 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in
-
 Methanothermus fervidus 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in
-
 Methanothermus fervidus ATCC 43054 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.86 CH3-H4MPT: CoM-SH methyltransferase complex
-
 Methanocaldococcus jannaschii
2.1.1.86 CH3-H4MPT: CoM-SH methyltransferase complex
-
 Methanothermus fervidus
2.1.1.86 methyl-tetrahydromethanopterin:coenzyme M methyltransferase complex
-
 Methanocaldococcus jannaschii
2.1.1.86 methyl-tetrahydromethanopterin:coenzyme M methyltransferase complex
-
 Methanothermus fervidus
2.1.1.86 MtrA-H complex
-
 Methanocaldococcus jannaschii
2.1.1.86 MtrA-H complex
-
 Methanothermus fervidus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.86 Cobalamin
-
 Methanocaldococcus jannaschii
2.1.1.86 Cobalamin
-
 Methanothermus fervidus