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Literature summary extracted from

  • Wu, Y.F.; Zhao, Y.; Liu, X.Y.; Gao, S.; Cheng, A.X.; Lou, H.X.
    Isolation and functional characterization of hydroxycinnamoyltransferases from the liverworts Plagiochasma appendiculatum and Marchantia paleacea (2018), Plant Physiol. Biochem., 129, 400-410 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.99 expression in Escherichia coli Plagiochasma appendiculatum
2.3.1.99 expression in Escherichia coli Marchantia paleacea
2.3.1.133 expression in Escherichia coli Plagiochasma appendiculatum
2.3.1.133 expression in Escherichia coli Marchantia paleacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.0102
-
 shikimate pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.99 0.0114
-
 shikimate pH 6.5, 30°C Marchantia paleacea
2.3.1.99 0.0295
-
 4-coumaroyl-CoA pH 6.5, 30°C Marchantia paleacea
2.3.1.99 0.0378
-
 4-coumaroyl-CoA pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.99 0.121
-
 quinate pH 6.5, 30°C Marchantia paleacea
2.3.1.99 0.133
-
 quinate pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.0102
-
 shikimate pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.0114
-
 shikimate pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.0295
-
 4-coumaroyl-CoA pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.0378
-
 4-coumaroyl-CoA pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.121
-
 quinate pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.133
-
 quinate pH 6.5, 30°C Plagiochasma appendiculatum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Marchantia paleacea A0A346FEE5
-
-
2.3.1.99 Plagiochasma appendiculatum A0A346FEE6
-
-
2.3.1.133 Marchantia paleacea A0A346FEE5
-
-
2.3.1.133 Plagiochasma appendiculatum A0A346FEE6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 0.0703
-
 substrates feruloyl-CoA and shikimate, pH 6.5, 30°C Marchantia paleacea
2.3.1.99 0.0715
-
 substrates caffeoyl-CoA and shikimate, pH 6.5, 30°C Marchantia paleacea
2.3.1.99 0.073
-
 substrates feruloyl-CoA and shikimate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.99 0.0737
-
 substrates caffeoyl-CoA and shikimate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.99 0.0807
-
 substrates 4-coumaroyl-CoA and shikimate, pH 6.5, 30°C Marchantia paleacea
2.3.1.99 0.081
-
 substrates 4-coumaroyl-CoA and shikimate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.99 0.0888
-
 substrates 4-coumaroyl-CoA and quinate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.99 0.0903
-
 substrates 4-coumaroyl-CoA and quinate, pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.0703
-
 substrates feruloyl-CoA and shikimate, pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.0715
-
 substrates caffeoyl-CoA and shikimate, pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.073
-
 substrates feruloyl-CoA and shikimate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.0736
-
 substrates caffeoyl-CoA and shikimate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.0807
-
 substrates 4-coumaroyl-CoA and shikimate, pH 6.5, 30°C Marchantia paleacea
2.3.1.133 0.0809
-
 substrates 4-coumaroyl-CoA and shikimate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.0888
-
 substrates 4-coumaroyl-CoA and quinate, pH 6.5, 30°C Plagiochasma appendiculatum
2.3.1.133 0.0903
-
 substrates 4-coumaroyl-CoA and quinate, pH 6.5, 30°C Marchantia paleacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 4-coumaroyl-CoA + quinate
-
 Plagiochasma appendiculatum CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 4-coumaroyl-CoA + quinate
-
 Marchantia paleacea CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.99 4-coumaroyl-CoA + shikimate reaction of EC 2.3.1.133 Plagiochasma appendiculatum CoA + 4-coumaroylshikimate
-
 ?
2.3.1.99 4-coumaroyl-CoA + shikimate reaction of EC 2.3.1.133 Marchantia paleacea CoA + 4-coumaroylshikimate
-
 ?
2.3.1.99 caffeoyl-CoA + shikimate reaction of EC 2.3.1.133 Plagiochasma appendiculatum CoA + 5-caffeoylshikimate
-
 ?
2.3.1.99 caffeoyl-CoA + shikimate reaction of EC 2.3.1.133 Marchantia paleacea CoA + 5-caffeoylshikimate
-
 ?
2.3.1.99 feruloyl-CoA + shikimate reaction of EC 2.3.1.133 Plagiochasma appendiculatum CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.99 feruloyl-CoA + shikimate reaction of EC 2.3.1.133 Marchantia paleacea CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.99 additional information no cosubstrates with quinate: caffeoyl-CoA, feruloyl-CoA Plagiochasma appendiculatum ?
-
-
2.3.1.99 additional information no cosubstrates with quinate: caffeoyl-CoA, feruloyl-CoA Marchantia paleacea ?
-
-
2.3.1.133 4-coumaroyl-CoA + quinate
-
 Plagiochasma appendiculatum CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + quinate
-
 Marchantia paleacea CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Plagiochasma appendiculatum CoA + 4-coumaroylshikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Marchantia paleacea CoA + 4-coumaroylshikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Plagiochasma appendiculatum CoA + 5-caffeoylshikimate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Marchantia paleacea CoA + 5-caffeoylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + shikimate
-
 Plagiochasma appendiculatum CoA + 5-O-feruloylshikimate
-
 ?
2.3.1.133 feruloyl-CoA + shikimate
-
 Marchantia paleacea CoA + 5-O-feruloylshikimate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.99 ? x * 60690, calculated from sequence, x * 81000, SDS-PAGE, recombinant protein including His-tag Marchantia paleacea
2.3.1.99 ? x * 61790, calculated from sequence, x * 81000, SDS-PAGE, recombinant protein including His-tag Plagiochasma appendiculatum
2.3.1.133 ? x * 60690, calculated from sequence, x * 81000, SDS-PAGE, recombinant protein including His-tag Marchantia paleacea
2.3.1.133 ? x * 61790, calculated from sequence, x * 81000, SDS-PAGE, recombinant protein including His-tag Plagiochasma appendiculatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.99 30
-
-
 Plagiochasma appendiculatum
2.3.1.99 30
-
-
 Marchantia paleacea
2.3.1.133 30
-
-
 Plagiochasma appendiculatum
2.3.1.133 30
-
-
 Marchantia paleacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.99 6.5
-
-
 Plagiochasma appendiculatum
2.3.1.99 6.5
-
-
 Marchantia paleacea
2.3.1.133 6.5
-
-
 Plagiochasma appendiculatum
2.3.1.133 6.5
-
-
 Marchantia paleacea

General Information

EC Number General Information Comment Organism
2.3.1.99 physiological function expression in Nicotiana tabacum causes a higher total production of chlorogenic acids Plagiochasma appendiculatum
2.3.1.99 physiological function expression in Nicotiana tabacum causes a higher total production of chlorogenic acids Marchantia paleacea
2.3.1.133 physiological function expression in Nicotiana tabacum causes a higher total production of chlorogenic acids Plagiochasma appendiculatum
2.3.1.133 physiological function expression in Nicotiana tabacum causes a higher total production of chlorogenic acids Marchantia paleacea