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Literature summary extracted from

  • Dong, J.; Wang, P.; Fu, X.; Dong, S.; Li, X.; Xiao, D.
    Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway (2019), J. Ind. Microbiol. Biotechnol., 46, 801-808 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.268 gene eat1, quantitative RT-PCR enzyme expression analysis in engineered strain CLy12a derivatives Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.268 additional information in Saccharomyces cerevisiae, the genes encoding phosphopantothenate-cysteine ligase, acetyl-CoA synthetase, and alcohol acetyltransferase are overexpressed by inserting the combined strong promoter PGK1p and the terminator PGK1t. The ethyl acetate levels of all engineered strains are enhanced. The final engineered strain CLy12a-ATF1-ACS2-CAB2 has an ethyl acetate yield of max. 610.26 mg/l, and the yield of higher alcohols is significantly decreased. The modification of ethyl acetate metabolic pathway is extremely important for the high level production of ethyl acetate in Saccharomyces cerevisiae Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.268 ethanol + acetyl-CoA Saccharomyces cerevisiae
-
 ethyl acetate + CoA
-
 ?
2.3.1.268 ethanol + acetyl-CoA Saccharomyces cerevisiae ATCC 204508
-
 ethyl acetate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.268 Saccharomyces cerevisiae P53208
-
-
2.3.1.268 Saccharomyces cerevisiae ATCC 204508 P53208
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.268 ethanol + acetyl-CoA
-
 Saccharomyces cerevisiae ethyl acetate + CoA
-
 ?
2.3.1.268 ethanol + acetyl-CoA
-
 Saccharomyces cerevisiae ATCC 204508 ethyl acetate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.268 alcohol acetyltransferase
-
 Saccharomyces cerevisiae
2.3.1.268 eat1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.268 30
-
 in vivo assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.268 acetyl-CoA
-
 Saccharomyces cerevisiae