Literature summary extracted from

Varland, S.; Myklebust, L.M.; Goksoeyr, S.O.; Glomnes, N.; Torsvik, J.; Varhaug, J.E.; Arnesen, T.
Identification of an alternatively spliced nuclear isoform of human N-terminal acetyltransferase Naa30 (2018), Gene, 644, 27-37 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.1.256	gene NAA30, DNA and amino acid sequence, analysis and comparison, alternative splicing variants NAA30_v1 (Naa30362) and NAA30_v2 (Naa30288), cloning from HeLa cells, overexpression of MBP-tagged Naa30362-V5 and Naa30288-V5 in HeLa cells	Homo sapiens

EC Number	Protein Variants	Comment	Organism
2.3.1.256	additional information	identification of a splice variant of human NAA30, which encodes a truncated protein named Naa30288. DNA sequencing reveals that the 900 bp product derived from alternative splicing results in a 222 bp deletion from the 3' end of exon 1 (c.550_771del). The truncated splice variant (867 bp) is otherwise identical to NAA30. Splicing NAA30_v2 translates into a truncated isoform (p.Val184_Leu257del) consisting of 288 amino acids (Naa30288). The splice variant is abundantly present in thyroid cancer tissues and in several different human cancer cell lines. Naa30288 localizes predominantly to the nucleus, as opposed to annotated Naa30 which has a cytoplasmic localization. Naa30288 is missing elements of the GNAT fold. Full-length Naa30 acetylates the classical NatC substrate peptide MLGTG in vitro, whereas no significant NAT activity is detected for Naa3028. Naa30288 appears to be enzymatically inactive. Overexpression of full-length Naa30362 increases cell viability via inhibition of apoptosis. In contrast, Naa30288 does not exert an anti-apoptotic effect	Homo sapiens

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.1.256	cytoplasm	full-length Naa30 (Naa30362)	Homo sapiens	5737	-
2.3.1.256	additional information	immunohistochemic detection in subcellular fractions	Homo sapiens	-	-
2.3.1.256	nucleus	alternatively spliced nuclear isoform Naa30288	Homo sapiens	5634	-

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.256	Homo sapiens	Q147X3	full-length splicing variant Naa30362	-

EC Number	Purification (Comment)	Organism
2.3.1.256	subcellular fractionation of HeLa cells and identification of splicing variants of Naa30	Homo sapiens

EC Number	Source Tissue	Comment	Organism	Textmining
2.3.1.256	8305C cell	-	Homo sapiens	-
2.3.1.256	A-431 cell	-	Homo sapiens	-
2.3.1.256	ARO cell	-	Homo sapiens	-
2.3.1.256	B-CPAP cell	-	Homo sapiens	-
2.3.1.256	BHT-101 cell	-	Homo sapiens	-
2.3.1.256	CAL-62 cell	-	Homo sapiens	-
2.3.1.256	CGTH-W1 cell	-	Homo sapiens	-
2.3.1.256	FTC-133 cell	-	Homo sapiens	-
2.3.1.256	HEK-293 cell	-	Homo sapiens	-
2.3.1.256	HeLa-S3 cell	-	Homo sapiens	-
2.3.1.256	MCF-7 cell	-	Homo sapiens	-
2.3.1.256	NPA cell	-	Homo sapiens	-
2.3.1.256	Nthy-ori 3-1 cell	-	Homo sapiens	-
2.3.1.256	ONCO-DG1 cell	-	Homo sapiens	-
2.3.1.256	peripheral blood mononuclear cell	primary	Homo sapiens	-
2.3.1.256	SH-SY5Y cell	-	Homo sapiens	-
2.3.1.256	T-84 cell	-	Homo sapiens	-
2.3.1.256	TAD-2 cell	-	Homo sapiens	-
2.3.1.256	thyroid cancer cell	high level of alternatively spliced nuclear isoform Naa30288	Homo sapiens	-
2.3.1.256	U-87MG cell	-	Homo sapiens	-

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.3.1.256	acetyl-CoA + N-terminal-L-methionyl-L-alanyl-[PLD]	a NatF-type substrate	Homo sapiens	N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[PLD] + CoA	-	?
2.3.1.256	acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[GPE]	a NatE-type substrate	Homo sapiens	N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[GPE] + CoA	-	?
2.3.1.256	acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[GTG]	a NatC-type substrate	Homo sapiens	N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[GTG] + CoA	-	?
2.3.1.256	additional information	full-length Naa30 acetylates a classical NatC substrate peptide in vitro, whereas no significant NAT activity is detected for Naa3028. Neither full-length Naa30 nor Naa30288 display any lysine acetyltransferase activity, and neither Naa30 isoforms have KAT activity towards histones. The SESSS, EEEIA and MDELF peptides are negative controls and represent NatA, Naa10 and NatB substrates, respectively. Poor activity with SGRGK peptide, which contains the natural 28-mer N-terminal sequence of histone H4, and represents a NatD-type substrate	Homo sapiens	?	-	-

EC Number	Subunits	Comment	Organism
2.3.1.256	?	x * 84000, recombinant MBP-tagged Naa30362, SDS-PAGE, x * 75000, recombinant MBP-Naa30288, SDS-PAGE	Homo sapiens

EC Number	Synonyms	Comment	Organism
2.3.1.256	N-terminal acetyltransferase Naa30	-	Homo sapiens
2.3.1.256	NAA30	-	Homo sapiens
2.3.1.256	NatC	-	Homo sapiens

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.1.256	37	-	assay at	Homo sapiens

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.1.256	7.4	-	assay at	Homo sapiens

EC Number	Cofactor	Comment	Organism	Structure
2.3.1.256	acetyl-CoA	-	Homo sapiens

EC Number	General Information	Comment	Organism
2.3.1.256	evolution	enzyme Naa30 belongs to the GNAT superfamily of acetyltransferases characterized by the highly conserved GNAT fold, which promotes Ac-CoA binding and substrate recognition	Homo sapiens
2.3.1.256	malfunction	overexpression of full-length Naa30 increases cell viability via inhibition of apoptosis. In contrast, Naa30288 does not exert an anti-apoptotic effect	Homo sapiens
2.3.1.256	additional information	homology structure modeling of Naa30 using the crystal structure of human Naa50 (PDB ID 3TFY) as template	Homo sapiens
2.3.1.256	physiological function	the NatC complex consists of the catalytic subunit Naa30 and the auxiliary subunits Naa35 and Naa38, and can potentially Nt-acetylate cytoplasmic proteins when the initiator methionine is followed by a bulky hydrophobic/amphipathic residue at position 2. Full-length enzyme Naa30362 improves cell viability and inhibits apoptosis	Homo sapiens