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Literature summary extracted from

  • Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.
    Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles (2015), Funct. Integr. Genomics, 15, 413-424 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.1 EDTA 1 mM, 1.3fold stimulation Fusarium proliferatum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression of the recombinant protein with His6-tag in Escherichia coli strain BL21 Fusarium proliferatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 Ag+ 1 mM, complete inhibition Fusarium proliferatum
3.5.5.1 Ba2+ 1 mM, 45% inhibition Fusarium proliferatum
3.5.5.1 Co2+ 1 mM, 35% inhibition Fusarium proliferatum
3.5.5.1 Cu2+ 1 mM, 30% inhibition Fusarium proliferatum
3.5.5.1 Dichloromethane 5% (v/v), 70% inhibition Fusarium proliferatum
3.5.5.1 ethanol 5% (v/v), 45% inhibition Fusarium proliferatum
3.5.5.1 hexane 5% (v/v), 80% inhibition Fusarium proliferatum
3.5.5.1 Li+ 1 mM, 20% inhibition Fusarium proliferatum
3.5.5.1 methanol 5% (v/v), 30% inhibition Fusarium proliferatum
3.5.5.1 Ni2+ 1 mM, 40% inhibition Fusarium proliferatum
3.5.5.1 Propanol 5% (v/v), 80% inhibition Fusarium proliferatum
3.5.5.1 Toluene 5% (v/v), 95% inhibition Fusarium proliferatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 1.55
-
 Benzonitrile pH 8.0, 37°C Fusarium proliferatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.1 Ca2+ 1 mM, 1.1.5fold stimulation Fusarium proliferatum
3.5.5.1 Fe2+ 1 mM, 1.1fold stimulation Fusarium proliferatum
3.5.5.1 Fe3+ 1 mM, 1.25fold stimulation Fusarium proliferatum
3.5.5.1 Mg2+ 1 mM, 1.25fold stimulation Fusarium proliferatum
3.5.5.1 Mn2+ 1 mM, 1.2fold stimulation Fusarium proliferatum
3.5.5.1 Zn2+ 1 mM, 1.2fold stimulation Fusarium proliferatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 37000
-
 SDS-PAGE Fusarium proliferatum

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Fusarium proliferatum A0A0E3D8K7
-
-
3.5.5.1 Fusarium proliferatum AUF-2 A0A0E3D8K7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
 Fusarium proliferatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 14.6
-
 pH 8.0, 37°C, substrate: benzonitrile Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2-cyanopyridine + 2 H2O 110% of the activity compared to benzonitrile Fusarium proliferatum pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 3-aminobutyronitrile + H2O 201% of the activity compared to benzonitrile Fusarium proliferatum 3-aminobutyric acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O 151% of the activity compared to benzonitrile Fusarium proliferatum pyridine-3-carboxylic acid + NH3
-
 ?
3.5.5.1 3-nitrobenzonitrile + H2O 110% of the activity compared to benzonitrile Fusarium proliferatum 3-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 3-phenylpropionitrile + H2O 151% of the activity compared to benzonitrile Fusarium proliferatum 3-phenylpropionate + NH3
-
 ?
3.5.5.1 4-aminobutyronitrile + H2O 251% of the activity compared to benzonitrile Fusarium proliferatum 4-aminobutyric acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O 225% of the activity compared to benzonitrile Fusarium proliferatum pyridine-4-carboxylic acid + NH3
-
 ?
3.5.5.1 4-nitrobenzonitrile + H2O 151% of the activity compared to benzonitrile Fusarium proliferatum 4-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 acetonitrile + H2O 135% of the activity compared to benzonitrile Fusarium proliferatum acetate + NH3
-
 ?
3.5.5.1 acetonitrile + H2O 135% of the activity compared to benzonitrile Fusarium proliferatum AUF-2 acetate + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O 400% of the activity compared to benzonitrile Fusarium proliferatum acrylate + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Fusarium proliferatum benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Fusarium proliferatum AUF-2 benzoic acid + NH3
-
 ?
3.5.5.1 butyronitrile + H2O 425% of the activity compared to benzonitrile Fusarium proliferatum butyrate + NH3
-
 ?
3.5.5.1 butyronitrile + H2O 425% of the activity compared to benzonitrile Fusarium proliferatum AUF-2 butyrate + NH3
-
 ?
3.5.5.1 di-phenylacetonitrile + H2O 72% of the activity compared to benzonitrile Fusarium proliferatum ? + NH3
-
 ?
3.5.5.1 glutaronitrile + H2O 319% of the activity compared to benzonitrile Fusarium proliferatum ? + NH3
-
 ?
3.5.5.1 mandelonitrile + H2O 69% of the activity compared to benzonitrile Fusarium proliferatum ? + NH3
-
 ?
3.5.5.1 additional information no activity with fumaronitrile, succinonitrile Fusarium proliferatum ?
-
-
3.5.5.1 additional information no activity with fumaronitrile, succinonitrile Fusarium proliferatum AUF-2 ?
-
-
3.5.5.1 propionitrile + H2O 416% of the activity compared to benzonitrile Fusarium proliferatum propionate + NH3
-
 ?
3.5.5.1 propionitrile + H2O 416% of the activity compared to benzonitrile Fusarium proliferatum AUF-2 propionate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 ? x * 37000, SDS-PAGE Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 35 40
-
 Fusarium proliferatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 20 45 20°C: about 40% of maximal activity, 45°C: about 60% of maximal activity Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 8
-
-
 Fusarium proliferatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 6 9.5 pH 6.0: about 65% of maximal activity, pH 9.5: about 65% of maximal activity Fusarium proliferatum