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Literature summary extracted from

  • Li, Y.; Zhong, W.; Koay, A.Z.; Ng, H.Q.; Koh-Stenta, X.; Nah, Q.; Lim, S.H.; Larsson, A.; Lescar, J.; Hill, J.; Dedon, P.C.; Kang, C.
    Backbone resonance assignment for the N-terminal region of bacterial tRNA-(N1G37) methyltransferase (2019), Biomol. NMR Assign., 13, 49-53 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228 gene trmD, recombinant expression of His-tagged isolated N-terminal domain in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.228 additional information the N-terminal domain is a useful construct to probe the molecular interactions with SAM competitive inhibitors, in the search for molecules with antibiotic activity Pseudomonas aeruginosa
2.1.1.228 sinefungin an active-site inhibitor and S-adenosyl-L-methionine (SAM) analogue, that binds at the N-terminal dommain in the SAM binding site. Structure analysis of crystallized isolated dimeric NTD, overview Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Pseudomonas aeruginosa
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Pseudomonas aeruginosa UCBPP-PA14
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Pseudomonas aeruginosa Q02RL6
-
-
2.1.1.228 Pseudomonas aeruginosa UCBPP-PA14 Q02RL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.228 recombinant His-tagged isolated N-terminal domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Pseudomonas aeruginosa S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Pseudomonas aeruginosa UCBPP-PA14 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 bacterial tRNA-(N1G37) methyltransferase
-
 Pseudomonas aeruginosa
2.1.1.228 TrmD
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.228 additional information
-
 metabolites such as S-adenosyl-L-methionine (SAM), S-adenosyl-L-homocysteine (SAH), and methylthioadenosine (MTA) enhance the thermostability of the enzyme's N-terminal domain by increasing its melting temperature (Tm) Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.228 S-adenosyl-L-methionine SAM Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.1.1.228 additional information the N-terminal domain (NTD) contains the S-adenosyl-L-methionine (SAM) binding region, this domain binds to SAM, S-adenosyl-L-homocysteine (SAH), and active-site inhibitors such as the SAM analogue sinefungin. Metabolites such as SAM, SAH and MTA enhance the thermostability of NTD by increasing its melting temperature (Tm), dynamics and ligand binding of NTD Pseudomonas aeruginosa