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Literature summary extracted from

  • Li, Y.; Zhong, W.; Koay, A.Z.; Ng, H.Q.; Nah, Q.; Wong, Y.H.; Hill, J.; Lescar, J.; Dedon, P.C.; Kang, C.
    Backbone resonance assignment for the full length tRNA-(N1G37) methyltransferase of Pseudomonas aeruginosa (2019), Biomol. NMR Assign., 13, 327-332 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.228 drug development bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) from Pseudomonas aeruginosa is a good target for development of antibacterial compounds Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228 gene trmD, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.228 sinefungin competitive inhibitor Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Pseudomonas aeruginosa
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Pseudomonas aeruginosa UCBPP-PA14
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Pseudomonas aeruginosa Q02RL6
-
-
2.1.1.228 Pseudomonas aeruginosa UCBPP-PA14 Q02RL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.228 recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Pseudomonas aeruginosa S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Pseudomonas aeruginosa UCBPP-PA14 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.228 dimer enzyme TrmD comprises two domains with the N-terminal domain binding to the S-adenosyl-L-methionine (SAM) cofactor and the C-terminal domain critical for tRNA binding. Bacterial TrmD is functional as a dimer Pseudomonas aeruginosa
2.1.1.228 More backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 TrmD
-
 Pseudomonas aeruginosa
2.1.1.228 tRNA-(N1G37) methyltransferase
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.228 8
-
 assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.228 S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.1.1.228 additional information backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis Pseudomonas aeruginosa
2.1.1.228 physiological function methylation of guanine at position 37 in RNA is critical for bacterial growth, as this reaction is essential for preventing reading frame shifts during translation and therefore for maintaining the fidelity of protein synthesis. This methylation is catalyzed by the tRNA (guanine37-N1)-methyltransferase (TrmD). TrmD uses S-adenosyl-l-methionine (SAM) as a cofactor and transfers the methyl group to the N1 atom of G37 in tRNA. Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) plays important roles in translation. and is critical for growth of Pseudomonas aeruginosa Pseudomonas aeruginosa