Literature summary extracted from

Zhong, W.; Koay, A.; Ngo, A.; Li, Y.; Nah, Q.; Wong, Y.H.; Chionh, Y.H.; Ng, H.Q.; Koh-Stenta, X.; Poulsen, A.; Foo, K.; McBee, M.; Choong, M.L.; El Sahili, A.; Kang, C.; Matter, A.; Lescar, J.; Hill, J.; Dedon, P.
Targeting the bacterial epitranscriptome for antibiotic development discovery of novel tRNA-(N1G37) methyltransferase (TrmD) inhibitors (2019), ACS Infect. Dis., 5, 326-335 .

Application

EC Number	Application	Comment	Organism
2.1.1.228	drug development	bacterial tRNA modification synthesis pathways are critical to cell survival under stress and thus represent ideal mechanism-based targets for antibiotic development. tRNA-(N1G37) methyltransferase (TrmD) is a target, which is conserved and essential in many bacterial pathogens	Pseudomonas aeruginosa

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.1.1.228	gene trmD, sequence comparisons	Pseudomonas aeruginosa

Inhibitors

EC Number	Inhibitors	Comment	Organism
2.1.1.228	(4-aminoquinazolin-2-yl)methyl 3-aminopyrazine-2-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	(4-oxo-3,4-dihydrothieno[3,2-d]pyrimidin-2-yl)methyl 2-(furan-2-yl)quinoline-4-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	1-(1H-pyrrol-2-yl)-2-[(thieno[2,3-d]pyrimidin-4-yl)oxy]ethan-1-one	-	Pseudomonas aeruginosa
2.1.1.228	1-(2-phenylpyrimidin-4-yl)-N-[(4-propoxyphenyl)methyl]piperidine-4-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	1-(3-chlorophenyl)-5-ethyl-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	1-[(2-chlorophenyl)methyl]-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	1-[7-(3,4-dimethylbenzoyl)-2H-[1,3]dioxolo[4,5-g]quinolin-8-yl]piperidine-4-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	2-(1H-inden-2-yl)-5-[2-(1H-indol-3-yl)ethyl]-4-methyl-1H-pyrazolo[4,3-c]pyridine-3,6(2H,5H)-dione	-	Pseudomonas aeruginosa
2.1.1.228	2-(5-chlorothiophen-2-yl)-2-oxoethyl (3,4-dimethoxyphenyl)acetate	-	Pseudomonas aeruginosa
2.1.1.228	2-(8-fluoro-3,4-dihydroquinolin-1(2H)-yl)-N-(quinolin-5-yl)acetamide	-	Pseudomonas aeruginosa
2.1.1.228	2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 2-methylquinoline-4-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 3-(cyclopropylsulfamoyl)thiophene-2-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	2-oxo-2-(3-sulfamoylanilino)ethyl 2-(furan-2-yl)quinoline-4-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	2-oxo-2-[(quinolin-5-yl)amino]ethyl 1-(5-carbamoylpyridin-2-yl)piperidine-4-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	2-phenyl-7-(quinoline-6-carbonyl)-5,6,7,8-tetrahydropyrazolo[1,5-a]pyrido[4,3-d]pyrimidin-9(1H)-one	-	Pseudomonas aeruginosa
2.1.1.228	2-[(3,5-dichloropyridin-2-yl)amino]-2-oxoethyl 5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	2-[(4-acetylbenzene-1-sulfonyl)amino]thiophene-3-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	2-[(5-chloropyridin-2-yl)amino]-2-oxoethyl 2-(furan-2-yl)quinoline-4-carboxylate	-	Pseudomonas aeruginosa
2.1.1.228	2-[2-(1-methyl-2-phenyl-2,3-dihydro-1H-indol-3-yl)-2-oxoethyl]-1,3-dioxo-1,2,3,5,6,7-hexahydropyrrolo[1,2-c]pyrimidine-4-carbonitrile	-	Pseudomonas aeruginosa
2.1.1.228	2-[2-(3-chlorophenoxy)acetamido]thiophene-3-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	2-[2-(4-oxocinnolin-1(4H)-yl)acetamido]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	2-[3-(benzenesulfonyl)-7-methyl-4-oxo-1,8-naphthyridin-1(4H)-yl]-N-methyl-N-phenylacetamide	-	Pseudomonas aeruginosa
2.1.1.228	2-[5-[2-(methylamino)-1,3-thiazol-4-yl]thiophen-2-yl]acetamide	-	Pseudomonas aeruginosa
2.1.1.228	3-(1H-pyrrol-1-yl)-N-[4-[(1,3-thiazol-2-yl)sulfamoyl]phenyl]benzamide	-	Pseudomonas aeruginosa
2.1.1.228	3-(4-methoxyphenyl)-7-[(1H-pyrrol-2-yl)methyl]-5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyrazine	-	Pseudomonas aeruginosa
2.1.1.228	3-oxo-N-(5,6,7,8-tetrahydronaphthalen-1-yl)-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	3-oxo-N-[1-[4-(1H-1,2,4-triazol-1-yl)phenyl]ethyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	3-oxo-N-[[2-(thiophen-2-yl)-1,3-oxazol-4-yl]methyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	4-[(tetrazolo[1,5-b]pyridazin-6-yl)amino]benzamide	-	Pseudomonas aeruginosa
2.1.1.228	4-[3-(8-hydroxyimidazo[1,2-a]pyridin-2-yl)-2,5-dimethyl-1H-pyrrol-1-yl]benzene-1-sulfonamide	-	Pseudomonas aeruginosa
2.1.1.228	4-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]thiomorpholine	-	Pseudomonas aeruginosa
2.1.1.228	5,7-dimethyl-N-(4H-1,2,4-triazol-3-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	5-(5-methylthiophen-2-yl)thieno[2,3-d]pyrimidin-4(3H)-one	-	Pseudomonas aeruginosa
2.1.1.228	5-[2-(1,4-dioxa-8-azaspiro[4.5]decan-8-yl)-2-oxoethyl]-2-(3-fluorophenyl)-7-methyl-3-(1H-pyrrol-1-yl)-2,3,3a,5-tetrahydro-4H-pyrazolo[3,4-d]pyridazin-4-one	-	Pseudomonas aeruginosa
2.1.1.228	6-(2-[[(furan-2-yl)methyl]amino]-1,3-thiazol-4-yl)-3,4-dihydroquinolin-2(1H)-one	-	Pseudomonas aeruginosa
2.1.1.228	6-[5-(thiophen-2-yl)-1,2,4-oxadiazol-3-yl][1,2,4]triazolo[4,3-a]pyridine	-	Pseudomonas aeruginosa
2.1.1.228	ethyl 4-[(3-cyano-6,7-dimethoxyquinolin-4-yl)amino]benzoate	-	Pseudomonas aeruginosa
2.1.1.228	additional information	high-throughput small-molecule library inhibitor screening, antibacterial growth inhibitory activities and haemolytic activity of selected TrmD inhibitors, binding affinity confirmed by thermal stability and surface plasmon resonance, overview	Pseudomonas aeruginosa
2.1.1.228	N-(2-methoxy-5-methylphenyl)-N'-[1,2,4]triazolo[4,3-a]pyridin-3-ylurea	-	Pseudomonas aeruginosa
2.1.1.228	N-(3,4-dimethylphenyl)-5,6-dihydro-4H-cyclopenta[d][1,2]oxazole-3-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-(3-acetylphenyl)-N2-[5-chloro-2-(pyrrolidin-1-yl)phenyl]glycinamide	-	Pseudomonas aeruginosa
2.1.1.228	N-(3-carbamoyl-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-chloropyridine-2-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-(3-carbamoylthiophen-2-yl)-2-(pyridin-4-yl)quinoline-4-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-(3-cyanothiophen-2-yl)-2-(furan-2-yl)quinoline-4-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-([1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-4-yl]methyl)-1H-indole-2-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-([2-[(morpholin-4-yl)methyl]phenyl]methyl)-2-(thiophene-2-carbonyl)benzamide	-	Pseudomonas aeruginosa
2.1.1.228	N-methyl-1-[2-([[2-(pyridin-3-yl)quinazolin-4-yl]amino]methyl)phenyl]methanesulfonamide	-	Pseudomonas aeruginosa
2.1.1.228	N-methyl-N-[(oxan-4-yl)methyl]-2-(thiophen-3-yl)acetamide	-	Pseudomonas aeruginosa
2.1.1.228	N-methyl-N-[[2-(pyrrolidin-1-yl)phenyl]methyl]-3-(1H-pyrrol-1-yl)benzamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[(3,5-dimethyl-1,2-oxazol-4-yl)methyl]quinoline-6-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-1H-indole-2-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-2-(thiophen-2-yl)acetamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[2-oxo-2-[(1,3,5-trimethyl-4,5-dihydro-1H-pyrazol-4-yl)amino]ethyl]-5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[3-(pyridin-4-yl)-1,2-oxazol-5-yl]-5,6,7,8-tetrahydronaphthalene-2-sulfonamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[3-([1,3]thiazolo[5,4-b]pyridin-2-yl)phenyl]quinoline-2-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[3-[(3-fluoro-4-methylphenyl)carbamoyl]phenyl]-1-([1,3]thiazolo[5,4-b]pyridin-2-yl)piperidine-3-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[4-(2-amino-2-oxoethyl)-1,3-thiazol-2-yl]-3-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)propanamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[4-chloro-3-(pyrrolidine-1-sulfonyl)phenyl]-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
2.1.1.228	N-[[4-(piperidine-1-carbonyl)phenyl]methyl]-2-(thiophene-2-carbonyl)benzamide	-	Pseudomonas aeruginosa
2.1.1.228	sinefungin	-	Pseudomonas aeruginosa
2.1.1.228	suramin	-	Pseudomonas aeruginosa
2.1.1.228	[4-(hydroxymethyl)-2,8-diazaspiro[4.5]decan-2-yl][1-(4-methoxyphenyl)cyclohexyl]methanone	-	Pseudomonas aeruginosa
2.1.1.228	[5-(furan-2-yl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-2-yl]methyl pyrazine-2-carboxylate	-	Pseudomonas aeruginosa

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1.1.228	0.0012	-	guanine37 in tRNALeu(CAG)	pH and temperature not specified in the publication	Pseudomonas aeruginosa
2.1.1.228	0.0018	-	S-adenosyl-L-methionine	pH and temperature not specified in the publication	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.1.1.228	S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
2.1.1.228	S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa UCBPP-PA14	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.1.228	Pseudomonas aeruginosa	Q02RL6	-	-
2.1.1.228	Pseudomonas aeruginosa UCBPP-PA14	Q02RL6	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.1.1.228	additional information	TrmD luminescence assay development	Pseudomonas aeruginosa	?	-	-
2.1.1.228	additional information	TrmD luminescence assay development	Pseudomonas aeruginosa UCBPP-PA14	?	-	-
2.1.1.228	S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
2.1.1.228	S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa UCBPP-PA14	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
2.1.1.228	S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)	-	?
2.1.1.228	S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)	-	Pseudomonas aeruginosa UCBPP-PA14	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.1.1.228	PaTrmD	-	Pseudomonas aeruginosa
2.1.1.228	TrmD	-	Pseudomonas aeruginosa
2.1.1.228	tRNA-(N1G37) methyltransferase	-	Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.1.1.228	additional information	-	thermostabilization of TrmD by compounds belonging to the pyridine-pyrazol-piperidin chemical cluster, overview	Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1.1.228	0.0105	-	S-adenosyl-L-methionine	pH and temperature not specified in the publication	Pseudomonas aeruginosa
2.1.1.228	0.0113	-	guanine37 in tRNALeu(CAG)	pH and temperature not specified in the publication	Pseudomonas aeruginosa

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.1.1.228	S-adenosyl-L-methionine	-	Pseudomonas aeruginosa

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
2.1.1.228	0.00011	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-[(2-chlorophenyl)methyl]-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide
2.1.1.228	0.00031	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-(1H-pyrrol-1-yl)-N-[4-[(1,3-thiazol-2-yl)sulfamoyl]phenyl]benzamide
2.1.1.228	0.00049	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	5-(5-methylthiophen-2-yl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.228	0.00053	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[(5-chloropyridin-2-yl)amino]-2-oxoethyl 2-(furan-2-yl)quinoline-4-carboxylate
2.1.1.228	0.0007	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[(3,5-dichloropyridin-2-yl)amino]-2-oxoethyl 5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxylate
2.1.1.228	0.00093	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-phenyl-7-(quinoline-6-carbonyl)-5,6,7,8-tetrahydropyrazolo[1,5-a]pyrido[4,3-d]pyrimidin-9(1H)-one
2.1.1.228	0.001	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[[4-(piperidine-1-carbonyl)phenyl]methyl]-2-(thiophene-2-carbonyl)benzamide
2.1.1.228	0.0011	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-(5-chlorothiophen-2-yl)-2-oxoethyl (3,4-dimethoxyphenyl)acetate
2.1.1.228	0.0013	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-([1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-4-yl]methyl)-1H-indole-2-carboxamide
2.1.1.228	0.0013	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-methyl-N-[[2-(pyrrolidin-1-yl)phenyl]methyl]-3-(1H-pyrrol-1-yl)benzamide
2.1.1.228	0.0014	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[2-(1-methyl-2-phenyl-2,3-dihydro-1H-indol-3-yl)-2-oxoethyl]-1,3-dioxo-1,2,3,5,6,7-hexahydropyrrolo[1,2-c]pyrimidine-4-carbonitrile
2.1.1.228	0.0015	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(3-sulfamoylanilino)ethyl 2-(furan-2-yl)quinoline-4-carboxylate
2.1.1.228	0.0016	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-carbamoyl-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-chloropyridine-2-carboxamide
2.1.1.228	0.0017	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	(4-oxo-3,4-dihydrothieno[3,2-d]pyrimidin-2-yl)methyl 2-(furan-2-yl)quinoline-4-carboxylate
2.1.1.228	0.0017	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-(3-chlorophenyl)-5-ethyl-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide
2.1.1.228	0.0017	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-(4-methoxyphenyl)-7-[(1H-pyrrol-2-yl)methyl]-5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyrazine
2.1.1.228	0.0019	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[2-(3-chlorophenoxy)acetamido]thiophene-3-carboxamide
2.1.1.228	0.0019	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	4-[(tetrazolo[1,5-b]pyridazin-6-yl)amino]benzamide
2.1.1.228	0.0019	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[4-(2-amino-2-oxoethyl)-1,3-thiazol-2-yl]-3-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)propanamide
2.1.1.228	0.002	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[5-[2-(methylamino)-1,3-thiazol-4-yl]thiophen-2-yl]acetamide
2.1.1.228	0.0021	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-cyanothiophen-2-yl)-2-(furan-2-yl)quinoline-4-carboxamide
2.1.1.228	0.0021	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[3-([1,3]thiazolo[5,4-b]pyridin-2-yl)phenyl]quinoline-2-carboxamide
2.1.1.228	0.0025	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[2-(4-oxocinnolin-1(4H)-yl)acetamido]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxamide
2.1.1.228	0.0025	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	5,7-dimethyl-N-(4H-1,2,4-triazol-3-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide
2.1.1.228	0.0027	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[3-(benzenesulfonyl)-7-methyl-4-oxo-1,8-naphthyridin-1(4H)-yl]-N-methyl-N-phenylacetamide
2.1.1.228	0.0028	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(2-methoxy-5-methylphenyl)-N'-[1,2,4]triazolo[4,3-a]pyridin-3-ylurea
2.1.1.228	0.0029	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[(4-acetylbenzene-1-sulfonyl)amino]thiophene-3-carboxamide
2.1.1.228	0.0029	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	4-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]thiomorpholine
2.1.1.228	0.0029	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-1H-indole-2-carboxamide
2.1.1.228	0.0031	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[4-chloro-3-(pyrrolidine-1-sulfonyl)phenyl]-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
2.1.1.228	0.0034	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(3-sulfamoylanilino)ethyl 2-(furan-2-yl)quinoline-4-carboxylate
2.1.1.228	0.0034	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-[(quinolin-5-yl)amino]ethyl 1-(5-carbamoylpyridin-2-yl)piperidine-4-carboxylate
2.1.1.228	0.0036	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	5-[2-(1,4-dioxa-8-azaspiro[4.5]decan-8-yl)-2-oxoethyl]-2-(3-fluorophenyl)-7-methyl-3-(1H-pyrrol-1-yl)-2,3,3a,5-tetrahydro-4H-pyrazolo[3,4-d]pyridazin-4-one
2.1.1.228	0.0038	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-(1H-inden-2-yl)-5-[2-(1H-indol-3-yl)ethyl]-4-methyl-1H-pyrazolo[4,3-c]pyridine-3,6(2H,5H)-dione
2.1.1.228	0.0039	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[(3,5-dimethyl-1,2-oxazol-4-yl)methyl]quinoline-6-carboxamide
2.1.1.228	0.0043	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-methyl-1-[2-([[2-(pyridin-3-yl)quinazolin-4-yl]amino]methyl)phenyl]methanesulfonamide
2.1.1.228	0.0045	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 2-methylquinoline-4-carboxylate
2.1.1.228	0.0047	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-acetylphenyl)-N2-[5-chloro-2-(pyrrolidin-1-yl)phenyl]glycinamide
2.1.1.228	0.0049	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-(1H-pyrrol-2-yl)-2-[(thieno[2,3-d]pyrimidin-4-yl)oxy]ethan-1-one
2.1.1.228	0.005	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3,4-dimethylphenyl)-5,6-dihydro-4H-cyclopenta[d][1,2]oxazole-3-carboxamide
2.1.1.228	0.0053	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 3-(cyclopropylsulfamoyl)thiophene-2-carboxylate
2.1.1.228	0.0053	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	[5-(furan-2-yl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-2-yl]methyl pyrazine-2-carboxylate
2.1.1.228	0.0054	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	6-[5-(thiophen-2-yl)-1,2,4-oxadiazol-3-yl][1,2,4]triazolo[4,3-a]pyridine
2.1.1.228	0.0056	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	4-[3-(8-hydroxyimidazo[1,2-a]pyridin-2-yl)-2,5-dimethyl-1H-pyrrol-1-yl]benzene-1-sulfonamide
2.1.1.228	0.0058	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-[7-(3,4-dimethylbenzoyl)-2H-[1,3]dioxolo[4,5-g]quinolin-8-yl]piperidine-4-carboxamide
2.1.1.228	0.0059	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	[4-(hydroxymethyl)-2,8-diazaspiro[4.5]decan-2-yl][1-(4-methoxyphenyl)cyclohexyl]methanone
2.1.1.228	0.006	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	ethyl 4-[(3-cyano-6,7-dimethoxyquinolin-4-yl)amino]benzoate
2.1.1.228	0.006	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-carbamoylthiophen-2-yl)-2-(pyridin-4-yl)quinoline-4-carboxamide
2.1.1.228	0.0062	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-methyl-N-[(oxan-4-yl)methyl]-2-(thiophen-3-yl)acetamide
2.1.1.228	0.0066	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-(8-fluoro-3,4-dihydroquinolin-1(2H)-yl)-N-(quinolin-5-yl)acetamide
2.1.1.228	0.0068	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[2-oxo-2-[(1,3,5-trimethyl-4,5-dihydro-1H-pyrazol-4-yl)amino]ethyl]-5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxamide
2.1.1.228	0.0083	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	sinefungin
2.1.1.228	0.0087	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-oxo-N-(5,6,7,8-tetrahydronaphthalen-1-yl)-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
2.1.1.228	0.0087	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[3-(pyridin-4-yl)-1,2-oxazol-5-yl]-5,6,7,8-tetrahydronaphthalene-2-sulfonamide
2.1.1.228	0.0091	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	suramin
2.1.1.228	0.0091	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-oxo-N-[1-[4-(1H-1,2,4-triazol-1-yl)phenyl]ethyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
2.1.1.228	0.0092	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	(4-aminoquinazolin-2-yl)methyl 3-aminopyrazine-2-carboxylate
2.1.1.228	0.0097	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-oxo-N-[[2-(thiophen-2-yl)-1,3-oxazol-4-yl]methyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
2.1.1.228	0.01	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-([2-[(morpholin-4-yl)methyl]phenyl]methyl)-2-(thiophene-2-carbonyl)benzamide
2.1.1.228	0.011	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	6-(2-[[(furan-2-yl)methyl]amino]-1,3-thiazol-4-yl)-3,4-dihydroquinolin-2(1H)-one
2.1.1.228	0.012	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-2-(thiophen-2-yl)acetamide
2.1.1.228	0.013	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-(2-phenylpyrimidin-4-yl)-N-[(4-propoxyphenyl)methyl]piperidine-4-carboxamide
2.1.1.228	0.014	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[3-[(3-fluoro-4-methylphenyl)carbamoyl]phenyl]-1-([1,3]thiazolo[5,4-b]pyridin-2-yl)piperidine-3-carboxamide

General Information

EC Number	General Information	Comment	Organism
2.1.1.228	additional information	active site structure in complex with S-adenosyl-L-methionine, overview	Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.1.1.228	5.83	-	S-adenosyl-L-methionine	pH and temperature not specified in the publication	Pseudomonas aeruginosa
2.1.1.228	9.44	-	guanine37 in tRNALeu(CAG)	pH and temperature not specified in the publication	Pseudomonas aeruginosa