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Literature summary extracted from

  • Liu, C.; Zuo, K.; Yu, H.; Sun, C.; Zhang, T.; Xu, L.; Jin, Y.; Im, W.; Jin, F.
    Preparation of minor ginsenosides C-Mx and C-K from notoginseng leaf ginsenosides by a special ginsenosidase type-I (2015), Process Biochem., 50, 2158-2167 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.193 additional information
-
 additional information Vmax is 79.6 mM/h for Rb1, 45.6 mM/h for Rb2, 1.62 mM/h for Rb3 to Rd, 10.8 mM/h for Rb3 to C-Mx1, and 6.16 mM/h for Rc Aspergillus niger
3.2.1.193 5.46
-
 ginsenoside Rc pH 5.0, 45°C Aspergillus niger
3.2.1.193 15.1
-
 ginsenoside Rb3 pH 5.0, 45°C, formation of compound Mx-1 Aspergillus niger
3.2.1.193 16.6
-
 ginsenoside Rb1 pH 5.0, 45°C Aspergillus niger
3.2.1.193 16.6
-
 ginsenoside Rb3 pH 5.0, 45°C, formation of ginsenoside Rd Aspergillus niger
3.2.1.193 20.4
-
 ginsenoside Rb2 pH 5.0, 45°C Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.193 compound Mc + H2O Aspergillus niger
-
 compound K + L-arabinofuranose
-
 ?
3.2.1.193 compound Mc1 + H2O Aspergillus niger
-
 compound Mc + D-glucose
-
 ?
3.2.1.193 compound Mx + H2O Aspergillus niger
-
 compound K + D-xylose
-
 ?
3.2.1.193 compound Mx-1 + H2O Aspergillus niger
-
 compound Mx + D-glucose
-
 ?
3.2.1.193 compound O + H2O Aspergillus niger
-
 compound Y + D-glucose
-
 ?
3.2.1.193 compound Y + H2O Aspergillus niger
-
 compound K + L-arabinopyranose
-
 ?
3.2.1.193 ginsenoside F2 + H2O Aspergillus niger
-
 compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb2 + H2O Aspergillus niger
-
 compound O + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb3 + H2O Aspergillus niger
-
 ginsenoside Rd + D-xylose
-
 ?
3.2.1.193 ginsenoside Rb3 + H2O Aspergillus niger
-
 compound Mx1 + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb3 + H2O Aspergillus niger g.848
-
 ginsenoside Rd + D-xylose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Aspergillus niger
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Aspergillus niger
-
 compound Mc1 + D-glucose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Aspergillus niger g.848
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rd + H2O Aspergillus niger
-
 ginsenoside F2 + D-glucose
-
 ?
3.2.1.193 additional information Aspergillus niger substrates are notoginseng leaf ginsenosides ?
-
 ?
3.2.1.193 additional information Aspergillus niger g.848 substrates are notoginseng leaf ginsenosides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.193 Aspergillus niger
-
-
-
3.2.1.193 Aspergillus niger g.848
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.193 native enzyme from mycelium by anion exchange chromatography to homogeneity Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.193 compound Mc + H2O
-
 Aspergillus niger compound K + L-arabinofuranose
-
 ?
3.2.1.193 compound Mc1 + H2O
-
 Aspergillus niger compound Mc + D-glucose
-
 ?
3.2.1.193 compound Mx + H2O
-
 Aspergillus niger compound K + D-xylose
-
 ?
3.2.1.193 compound Mx-1 + H2O
-
 Aspergillus niger compound Mx + D-glucose
-
 ?
3.2.1.193 compound O + H2O
-
 Aspergillus niger compound Y + D-glucose
-
 ?
3.2.1.193 compound Y + H2O
-
 Aspergillus niger compound K + L-arabinopyranose
-
 ?
3.2.1.193 ginsenoside F2 + H2O
-
 Aspergillus niger compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb1 + H2O
-
 Aspergillus niger ginsenoside Rd + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb1 + H2O
-
 Aspergillus niger g.848 ginsenoside Rd + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb2 + H2O
-
 Aspergillus niger compound O + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb3 + H2O
-
 Aspergillus niger ginsenoside Rd + D-xylose
-
 ?
3.2.1.193 ginsenoside Rb3 + H2O
-
 Aspergillus niger g.848 ginsenoside Rd + D-xylose
-
 ?
3.2.1.193 ginsenoside Rb3 + H2O
-
 Aspergillus niger compound Mx1 + D-glucose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Aspergillus niger ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Aspergillus niger g.848 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Aspergillus niger compound Mc1 + D-glucose
-
 ?
3.2.1.193 ginsenoside Rd + H2O
-
 Aspergillus niger ginsenoside F2 + D-glucose
-
 ?
3.2.1.193 additional information substrates are notoginseng leaf ginsenosides Aspergillus niger ?
-
 ?
3.2.1.193 additional information the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I Aspergillus niger ?
-
 ?
3.2.1.193 additional information substrates are notoginseng leaf ginsenosides Aspergillus niger g.848 ?
-
 ?
3.2.1.193 additional information the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I Aspergillus niger g.848 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.193 ? x * 75000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.193 ginsenosidase type-I
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.193 45
-
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.193 5
-
-
 Aspergillus niger