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Literature summary extracted from

  • Yang, W.; Tang, H.; Ni, J.; Wu, Q.; Hua, D.; Tao, F.; Xu, P.
    Characterization of two Streptomyces enzymes that convert ferulic acid to vanillin (2013), PLoS One, 8, e67339 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.61 expression in Escherichia coli Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.61 32000
-
 SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.61 feruloyl-CoA + H2O Streptomyces sp.
-
 vanillin + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.61 Streptomyces sp. S5LPF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.61 His6-tagged enzyme Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
-
 Streptomyces sp. vanillin + acetyl-CoA
-
 ?
4.1.2.61 feruloyl-CoA + H2O
-
 Streptomyces sp. vanillin + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.61 ? x * 32000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 ECH
-
 Streptomyces sp.
4.1.2.61 enoyl-CoA-hydratase/aldolase
-
 Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.61 NAD+ the enzyme binds an NAD+ cofactor Streptomyces sp.

General Information

EC Number General Information Comment Organism
4.1.2.61 metabolism the enzyme is involved in the vanillin production from ferulic acid Streptomyces sp.