Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Handali, M.; Neupane, D.; Roychowdhury, H.; Yukl, E.
    Transcriptional regulation, metal binding properties and structure of Pden1597, an unusual zinc transport protein from Paracoccus denitrificans (2015), J. Biol. Chem., 290, 11878-11889 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.20 expressed in Escherichia coli BL21(DE3) cells Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.20 Paracoccus denitrificans
-
-
-
7.2.2.20 Paracoccus denitrificans Pd1222
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.20 ammonium sulfate precipitation and Sephacryl S-100 gel filtration Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.20 ATP + H2O + Mn2+-[manganese-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans ADP + phosphate + Mn2+[side2] + [manganese-binding protein][side2]
-
 ?
7.2.2.20 ATP + H2O + Mn2+-[manganese-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans Pd1222 ADP + phosphate + Mn2+[side2] + [manganese-binding protein][side2]
-
 ?
7.2.2.20 ATP + H2O + Zn2+-[zinc-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans ADP + phosphate + Zn2+[side2] + [zinc-binding protein][side2]
-
 ?
7.2.2.20 ATP + H2O + Zn2+-[zinc-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans Pd1222 ADP + phosphate + Zn2+[side2] + [zinc-binding protein][side2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.20 Pden1597
-
 Paracoccus denitrificans
7.2.2.20 Zn2+-specific transporter
-
 Paracoccus denitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.2.2.20 90
-
 the Zn2+-bound protein is remarkably stable, maintaining its circular dichroism spectrum up to 90°C Paracoccus denitrificans

Expression

EC Number Organism Comment Expression
7.2.2.20 Paracoccus denitrificans enzyme expression is increased under conditions of Zn2+ starvation up