Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhou, Y.; Xie, G.; Chang, L.; Wang, Y.; Gao, R.
    Identification of an archaeal maltooligosyltrehalose trehalohydrolase encoded by an interrupted gene (2017), Extremophiles, 21, 537-549 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.141 additional information poor effect by EDTA Sulfurisphaera tokodaii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.141 gene ST0926, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes Sulfurisphaera tokodaii
3.2.1.141 gene ST0927, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes Sulfurisphaera tokodaii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.141 additional information generation of a specific deletion mutation, designed on the basis of sequence alignments of the adjacent ORFs and the published Sulfolobus sp. MTHases, allowing soluble expression in Escherichia coli as active acidic and thermophilic enzyme Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.141 Fe3+
-
 Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.141 Mn2+ activates slightly Sulfurisphaera tokodaii
3.2.1.141 additional information no or poor effects by Na+, K+, Ca2+, Mg2+, Cu2+, Zn2+, and Ni2+ Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.141 Sulfurisphaera tokodaii Q973H4 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii Q973H5 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii 7 Q973H4 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii 7 Q973H5 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii DSM 16993 Q973H4 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii DSM 16993 Q973H5 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii JCM 10545 Q973H4 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii JCM 10545 Q973H5 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii NBRC 100140 Q973H4 i.e. Sulfolobus tokodaii
-
3.2.1.141 Sulfurisphaera tokodaii NBRC 100140 Q973H5 i.e. Sulfolobus tokodaii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.141 recombinant His6-tagged enzyme mutant from Escherichia coli by nickel affinity chromatography, followed by anion exchange chromatography, and multiple dialysis steps Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii ?
-
 ?
3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii 7 ?
-
 ?
3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii DSM 16993 ?
-
 ?
3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii JCM 10545 ?
-
 ?
3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii NBRC 100140 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.141 ? x * 65000, about, putative mature StMTHase protein lacking the N-terminal Trx-tag and His6-tag, sequence calculation Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.141 maltooligosyltrehalose trehalohydrolase
-
 Sulfurisphaera tokodaii
3.2.1.141 ST0926
-
 Sulfurisphaera tokodaii
3.2.1.141 ST0927
-
 Sulfurisphaera tokodaii
3.2.1.141 StMTHase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.141 70
-
 recombinant enzyme mutant Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.141 60 80 over 90% of maximal activity within this range, recombinant enzyme mutant Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.141 5
-
 recombinant enzyme mutant Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.141 3.5 6 50% of maximal activity within this range, recombinant enzyme mutant Sulfurisphaera tokodaii

General Information

EC Number General Information Comment Organism
3.2.1.141 additional information prediction analysis of putative structural domains of ST0926/ ST0927 and modeling using crystal structure, PDB ID 1EH9, of Sulfurisphaera tokodaii KM1 MTHase as template Sulfurisphaera tokodaii