Literature summary extracted from

Shin, K.; Oh, D.
Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides (2016), Crit. Rev. Biotechnol., 36, 1036-1049 .

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
3.2.1.B23	ginsenoside C-K + H2O	Rhizobium sp.	-	protopanaxadiol + D-glucose	-	?
3.2.1.B23	ginsenoside C-O + H2O	Bifidobacterium choerinum	-	ginsenoside F2 + L-arabinopyranose	-	?
3.2.1.B23	ginsenoside G17 + H2O	Thermus thermophilus	-	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G17 + H2O	Bifidobacterium longum	-	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G17 + H2O	Flavobacterium johnsoniae	-	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G17 + H2O	Bifidobacterium longum H-1	-	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G75 + H2O	Thermus thermophilus	-	ginsenoside C-K + D-glucose	-	?
3.2.1.B23	ginsenoside Mc + H2O	Caldicellulosiruptor saccharolyticus	-	ginsenoside C-K + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Mc + H2O	Rhodanobacter ginsenosidimutans	-	ginsenoside C-K + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Mc1 + H2O	Rhodanobacter ginsenosidimutans	-	ginsenoside F2 + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Thermus thermophilus	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Trichoderma viride	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Aspergillus sp.	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Bifidobacterium longum	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Lissachatina fulica	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Fulvia fulva	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Pseudothermotoga thermarum	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Flavobacterium johnsoniae	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Aspergillus niger	-	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Pseudonocardia sp.	-	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Microbacterium esteraromaticum	-	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Bifidobacterium longum H-1	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	Pseudothermotoga thermarum DSM 5069T	-	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	Trichoderma viride	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	Bifidobacterium longum	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	Bifidobacterium choerinum	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	Microbacterium esteraromaticum	-	ginsenoside Rg3 + D-glucose + L-arabinopyranose	-	?
3.2.1.B23	ginsenoside Rb3 + H2O	Pseudonocardia sp.	-	ginsenoside Rg3 + D-glucose + D-xylose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Bifidobacterium breve	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Trichoderma viride	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Caldicellulosiruptor saccharolyticus	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Bifidobacterium longum	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Panax ginseng	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Leuconostoc sp.	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Rhodanobacter ginsenosidimutans	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	Bifidobacterium longum H-1	-	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rd + H2O	Pseudonocardia sp.	-	ginsenoside Rg3 + D-glucose	-	?
3.2.1.B23	ginsenoside Re + H2O	Mucilaginibacter sp. QM49	-	ginsenoside Rg2 + D-glucose	-	?
3.2.1.B23	ginsenoside Rg1 + H2O	Mucilaginibacter sp. QM49	-	ginsenoside Rh1 + D-glucose	-	?
3.2.1.B23	additional information	Thermus thermophilus	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Trichoderma viride	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Aspergillus sp.	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Bifidobacterium longum	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Lissachatina fulica	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Fulvia fulva	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Pseudothermotoga thermarum	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Flavobacterium johnsoniae	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Bifidobacterium breve	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	?	-	?
3.2.1.B23	additional information	Trichoderma viride	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	?	-	?
3.2.1.B23	additional information	Caldicellulosiruptor saccharolyticus	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	?	-	?
3.2.1.B23	additional information	Bifidobacterium longum	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	?	-	?
3.2.1.B23	additional information	Bifidobacterium choerinum	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	?	-	?
3.2.1.B23	additional information	Bifidobacterium longum H-1	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.B23	additional information	Bifidobacterium longum H-1	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	?	-	?
3.2.1.B23	additional information	Pseudothermotoga thermarum DSM 5069T	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	?	-	?
3.2.1.191	ginsenoside Rb1 + H2O	Sphingomonas sp.	-	ginsenoside G17 + D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	Sphingopyxis alaskensis	-	ginsenoside G17 + D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	Esteya vermicola	via ginsenoside G17	ginsenoside G75 + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	Esteya vermicola CNU120806	via ginsenoside G17	ginsenoside G75 + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rb2 + H2O	Sphingomonas sp.	-	ginsenoside C-O + D-glucose	-	?
3.2.1.191	ginsenoside Rb2 + H2O	Sphingopyxis alaskensis	-	ginsenoside C-O + D-glucose	-	?
3.2.1.191	ginsenoside Rc + H2O	Sphingomonas sp.	-	ginsenoside C-Mc1 + D-glucose	-	?
3.2.1.191	ginsenoside Rc + H2O	Sphingopyxis alaskensis	-	ginsenoside C-Mc1 + D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	Esteya vermicola	via ginsenosside F2	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	Sphingomonas sp.	-	ginsenoside F2 + D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	Sphingopyxis alaskensis	-	ginsenoside F2 + D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	Esteya vermicola CNU120806	via ginsenosside F2	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	Fusarium sp.	-	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	Fusarium proliferatum	-	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	Panax ginseng	-	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	Sphingopyxis alaskensis	-	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	Fusarium proliferatum ECU2042	-	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	additional information	Aspergillus niger	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Fusarium sp.	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Sphingomonas sp.	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Fusarium proliferatum	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Panax ginseng	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Terrabacter ginsenosidimutans	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Sphingopyxis alaskensis	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Esteya vermicola	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Esteya vermicola CNU120806	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.191	additional information	Fusarium proliferatum ECU2042	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	?	-	?
3.2.1.193	ginsenoside F2 + H2O	Sphingomonas leidyi	-	ginsenoside C-K + D-glucose	-	?
3.2.1.193	ginsenoside F2 + H2O	Aspergillus oryzae	-	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Saccharolobus solfataricus	via ginsenoside F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Aspergillus sp.	via ginsenoside F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Leuconostoc citreum	via ginsenoside F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Sulfolobus acidocaldarius	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Leuconostoc mesenteroides	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Lactiplantibacillus pentosus	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Microbacterium esteraromaticum	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Fusobacterium sp.	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Companilactobacillus paralimentarius	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Sphingomonas leidyi	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Aspergillus niger	via ginsenosides Rd and F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Aspergillus oryzae	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Pseudarthrobacter chlorophenolicus	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Intrasporangium sp.	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Globisporangium irregulare	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Nocardioides panaciterrulae	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Sphingomonas leidyi	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Paecilomyces variotii	-	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Citrus limon	-	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Aspergillus sp.	via ginsenoside F2	ginsenoside Rh2 + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Lactiplantibacillus pentosus 6105	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Intrasporangium sp. GS603	-	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Leuconostoc mesenteroides DC102	via ginsenosides F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Leuconostoc citreum LH1	via ginsenoside F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Aspergillus niger g.848	via ginsenosides Rd and F2	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	Paecilomyces variotii 229-7	-	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Aspergillus oryzae	-	ginsenoside F2 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Pseudarthrobacter chlorophenolicus	-	ginsenoside F2 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Globisporangium irregulare	-	ginsenoside F2 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Sulfolobus acidocaldarius	-	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Microbacterium esteraromaticum	-	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Saccharolobus solfataricus	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Aspergillus sp.	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Aspergillus niger	via ginsenosides Rd and F2	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Citrus limon	-	ginsenoside Rg3 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Aspergillus sp.	via ginsenoside F2	ginsenoside Rh2 + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	Aspergillus niger g.848	via ginsenosides Rd and F2	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb3 + H2O	Aspergillus niger	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + xylose	-	?
3.2.1.193	ginsenoside Rb3 + H2O	Aspergillus niger g.848	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + xylose	-	?
3.2.1.193	ginsenoside Rc + H2O	Aspergillus oryzae	-	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Pseudarthrobacter chlorophenolicus	-	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Globisporangium irregulare	-	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Nocardioides panaciterrulae	-	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Aspergillus niger	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Saccharolobus solfataricus	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Aspergillus sp.	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Citrus limon	-	ginsenoside Rg3 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Aspergillus sp.	via ginsenoside F2	ginsenoside Rh2 + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	Aspergillus niger g.848	via ginsenoside F2	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rd + H2O	Paecilomyces variotii	-	ginsenoside Rg3 + D-glucose	-	?
3.2.1.193	ginsenoside Rd + H2O	Paecilomyces variotii 229-7	-	ginsenoside Rg3 + D-glucose	-	?
3.2.1.193	additional information	Aspergillus niger	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Paecilomyces variotii	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Sulfolobus acidocaldarius	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Leuconostoc mesenteroides	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Lactiplantibacillus pentosus	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Saccharolobus solfataricus	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Aspergillus oryzae	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Aspergillus sp.	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Microbacterium esteraromaticum	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Citrus limon	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Leuconostoc citreum	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Fusobacterium sp.	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Pseudarthrobacter chlorophenolicus	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Intrasporangium sp.	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Globisporangium irregulare	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Nocardioides panaciterrulae	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Companilactobacillus paralimentarius	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Sphingomonas leidyi	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Lactiplantibacillus pentosus 6105	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Intrasporangium sp. GS603	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Leuconostoc mesenteroides DC102	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Leuconostoc citreum LH1	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Aspergillus niger g.848	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.193	additional information	Paecilomyces variotii 229-7	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	?	-	?
3.2.1.194	ginsenoside R1 + H2O	Aspergillus sp.	-	ginsenoside F1 + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Re + H2O	Pyrococcus furiosus	-	ginsenoside Rg1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Re + H2O	Aspergillus sp.	-	ginsenoside F1 + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Re + H2O	Penicillium decumbens	-	ginsenoside F1 + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Rf + H2O	Aspergillus niger	-	ginsenoside Rh1 + D-glucose	-	?
3.2.1.194	ginsenoside Rf + H2O	Pyrococcus furiosus	-	ginsenoside Rh1 + D-glucose	-	?
3.2.1.194	ginsenoside Rf + H2O	Aspergillus sp.	-	protopanaxatriol + 2 D-glucose	-	?
3.2.1.194	ginsenoside Rf + H2O	Penicillium decumbens	-	protopanaxatriol + 2 D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	Penicillium decumbens	-	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	Fusarium subglutinans	-	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	Penicillium sclerotiorum	-	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	Sanguibacter keddieii	-	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg2 + 2 H2O	Penicillium decumbens	-	protopanaxatriol + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	Pyrococcus furiosus	-	ginsenoside Rh1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	Thermoanaerobacterium thermosaccharolyticum	-	ginsenoside Rh1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	Absidia sp. (in: Fungi)	-	ginsenoside Rh1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	Aspergillus sp.	-	protopanaxatriol + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Rh1 + H2O	Penicillium decumbens	-	protopanaxatriol + D-glucose	-	?
3.2.1.194	additional information	Pyrococcus furiosus	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	?	-	?
3.2.1.194	additional information	Aspergillus niger	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Aspergillus niger	?	-	?
3.2.1.194	additional information	Absidia sp. (in: Fungi)	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	?	-	?
3.2.1.194	additional information	Pyrococcus furiosus	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion was performed using b-glycosidase from Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	Pyrococcus furiosus	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
3.2.1.194	additional information	Thermoanaerobacterium thermosaccharolyticum	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
3.2.1.194	additional information	Penicillium decumbens	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
3.2.1.194	additional information	Fusarium subglutinans	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
3.2.1.194	additional information	Penicillium sclerotiorum	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
3.2.1.194	additional information	Sanguibacter keddieii	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	?	-	?
3.2.1.194	additional information	Aspergillus sp.	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3	?	-	?
3.2.1.194	notoginsenoside R1 + H2O	Pyrococcus furiosus	-	ginsenoside Rg1 + D-xylose	-	?
3.2.1.194	notoginsenoside R1 + H2O	Thermoanaerobacterium thermosaccharolyticum	-	ginsenoside Rg1 + D-xylose	-	?
3.2.1.195	ginsenoside Re + H2O	Penicillium sp.	-	ginsenoside Rh1 + D-glucose + L-rhamnose	-	?
3.2.1.195	ginsenoside Re + H2O	Actinosynnema mirum	-	ginsenoside Rh1 + D-glucose + L-rhamnose	-	?
3.2.1.195	ginsenoside Rg1 + H2O	Penicillium sp.	via ginsenoside Rh1	protopanaxatriol + 2 D-glucose	-	?
3.2.1.195	ginsenoside Rg1 + H2O	Actinosynnema mirum	via ginsenoside Rh1	protopanaxatriol + 2 D-glucose	-	?
3.2.1.195	additional information	Penicillium sp.	ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively	?	-	?
3.2.1.195	additional information	Actinosynnema mirum	ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.B23	Aspergillus niger	-	-	-
3.2.1.B23	Aspergillus sp.	-	-	-
3.2.1.B23	Bifidobacterium breve	-	-	-
3.2.1.B23	Bifidobacterium choerinum	-	-	-
3.2.1.B23	Bifidobacterium longum	-	-	-
3.2.1.B23	Bifidobacterium longum H-1	-	-	-
3.2.1.B23	Caldicellulosiruptor saccharolyticus	-	-	-
3.2.1.B23	Flavobacterium johnsoniae	-	-	-
3.2.1.B23	Fulvia fulva	-	-	-
3.2.1.B23	Leuconostoc sp.	-	-	-
3.2.1.B23	Lissachatina fulica	-	-	-
3.2.1.B23	Microbacterium esteraromaticum	-	-	-
3.2.1.B23	Mucilaginibacter sp. QM49	-	-	-
3.2.1.B23	Panax ginseng	-	-	-
3.2.1.B23	Pseudonocardia sp.	-	-	-
3.2.1.B23	Pseudothermotoga thermarum	-	-	-
3.2.1.B23	Pseudothermotoga thermarum DSM 5069T	-	-	-
3.2.1.B23	Rhizobium sp.	-	-	-
3.2.1.B23	Rhodanobacter ginsenosidimutans	-	-	-
3.2.1.B23	Thermus thermophilus	-	-	-
3.2.1.B23	Trichoderma viride	-	-	-
3.2.1.191	Aspergillus niger	-	-	-
3.2.1.191	Esteya vermicola	-	-	-
3.2.1.191	Esteya vermicola CNU120806	-	-	-
3.2.1.191	Fusarium proliferatum	-	-	-
3.2.1.191	Fusarium proliferatum ECU2042	-	-	-
3.2.1.191	Fusarium sp.	-	-	-
3.2.1.191	Panax ginseng	-	-	-
3.2.1.191	Sphingomonas sp.	-	-	-
3.2.1.191	Sphingopyxis alaskensis	-	-	-
3.2.1.191	Terrabacter ginsenosidimutans	-	-	-
3.2.1.193	Aspergillus niger	-	-	-
3.2.1.193	Aspergillus niger g.848	-	-	-
3.2.1.193	Aspergillus oryzae	-	-	-
3.2.1.193	Aspergillus sp.	-	-	-
3.2.1.193	Citrus limon	-	-	-
3.2.1.193	Companilactobacillus paralimentarius	-	-	-
3.2.1.193	Fusobacterium sp.	-	-	-
3.2.1.193	Globisporangium irregulare	-	-	-
3.2.1.193	Intrasporangium sp.	-	-	-
3.2.1.193	Intrasporangium sp. GS603	-	-	-
3.2.1.193	Lactiplantibacillus pentosus	-	-	-
3.2.1.193	Lactiplantibacillus pentosus 6105	-	-	-
3.2.1.193	Leuconostoc citreum	-	isolated from kimchi	-
3.2.1.193	Leuconostoc citreum LH1	-	isolated from kimchi	-
3.2.1.193	Leuconostoc mesenteroides	-	-	-
3.2.1.193	Leuconostoc mesenteroides DC102	-	-	-
3.2.1.193	Microbacterium esteraromaticum	-	-	-
3.2.1.193	Nocardioides panaciterrulae	-	sp. nov., isolated from soil of a ginseng field	-
3.2.1.193	Paecilomyces variotii	-	i.e. Paecilomyces bainier	-
3.2.1.193	Paecilomyces variotii 229-7	-	i.e. Paecilomyces bainier	-
3.2.1.193	Pseudarthrobacter chlorophenolicus	-	-	-
3.2.1.193	Saccharolobus solfataricus	-	-	-
3.2.1.193	Sphingomonas leidyi	-	-	-
3.2.1.193	Sulfolobus acidocaldarius	-	-	-
3.2.1.194	Absidia sp. (in: Fungi)	-	-	-
3.2.1.194	Aspergillus niger	-	-	-
3.2.1.194	Aspergillus sp.	-	-	-
3.2.1.194	Fusarium subglutinans	-	-	-
3.2.1.194	Penicillium decumbens	-	-	-
3.2.1.194	Penicillium sclerotiorum	-	-	-
3.2.1.194	Pyrococcus furiosus	-	-	-
3.2.1.194	Sanguibacter keddieii	-	-	-
3.2.1.194	Thermoanaerobacterium thermosaccharolyticum	-	-	-
3.2.1.195	Actinosynnema mirum	-	-	-
3.2.1.195	Penicillium sp.	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.191	native enzyme	Esteya vermicola

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2.1.B23	3	-	pH 5.0, 50°C	Panax ginseng
3.2.1.B23	4	-	pH 8.0, 30°C	Mucilaginibacter sp. QM49
3.2.1.B23	5	-	pH 6.5, 90°C	Thermus thermophilus
3.2.1.B23	8	-	pH 6.8, 37°C	Bifidobacterium longum
3.2.1.B23	9	-	pH 5.5, 37°C	Bifidobacterium breve
3.2.1.B23	15	-	pH 7.5, 37°C	Rhodanobacter ginsenosidimutans
3.2.1.B23	18	-	pH 6.0, 45°C	Fulvia fulva
3.2.1.B23	22	-	pH 5.5, 35°C	Bifidobacterium longum
3.2.1.B23	24	-	pH 5.5, 45°C	Fulvia fulva
3.2.1.B23	26	-	pH 6.0, 37°C	Leuconostoc sp.
3.2.1.B23	28	-	pH 5.5, 80°C	Caldicellulosiruptor saccharolyticus
3.2.1.B23	40	-	pH 7.0, 37°C	Pseudonocardia sp.
3.2.1.B23	61	-	pH 7.0, 40°C	Microbacterium esteraromaticum
3.2.1.B23	67	-	pH 4.7, 37°C	Bifidobacterium longum
3.2.1.B23	140	-	pH 4.8, 90°C	Pseudothermotoga thermarum
3.2.1.B23	250	-	pH 5.6, 70°C	Lissachatina fulica
3.2.1.191	2	-	pH 5.0, 60°C, substrate ginsenoside Rg3	Panax ginseng
3.2.1.191	76	-	purified enzyme, pH 7.0, 37°C	Terrabacter ginsenosidimutans
3.2.1.191	290	-	purified native enzyme, pH 5.0, 50°C	Esteya vermicola
3.2.1.191	309	-	pH 5.5, 40°C	Sphingopyxis alaskensis
3.2.1.193	29	-	pH 7.0, 40°C	Microbacterium esteraromaticum
3.2.1.193	137	-	pH 3.5, 60°C	Paecilomyces variotii
3.2.1.193	2020	-	pH 5.5, 90°C	Saccharolobus solfataricus
3.2.1.194	1	-	pH 5.0, 40°C	Absidia sp. (in: Fungi)
3.2.1.194	2	-	pH 5.5, 95°C	Pyrococcus furiosus
3.2.1.194	2	-	substrate ginsenoside Rf, pH 6.0, 40°C	Aspergillus sp.
3.2.1.194	4	-	substrate ginsenoside Rf, pH 7.5, 37°C	Aspergillus niger
3.2.1.194	4.5	-	pH 6.5, 60°C	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	136	-	pH 6.0, 50°C	Fusarium subglutinans
3.2.1.194	136	-	pH 6.0, 50°C	Penicillium sclerotiorum
3.2.1.194	912	-	pH 6.0, 40°C	Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3.2.1.B23	ginsenoside C-K + H2O	-	Rhizobium sp.	protopanaxadiol + D-glucose	-	?
3.2.1.B23	ginsenoside C-O + H2O	-	Bifidobacterium choerinum	ginsenoside F2 + L-arabinopyranose	-	?
3.2.1.B23	ginsenoside G17 + H2O	-	Thermus thermophilus	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G17 + H2O	-	Bifidobacterium longum	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G17 + H2O	-	Flavobacterium johnsoniae	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G17 + H2O	-	Bifidobacterium longum H-1	ginsenoside F2 + D-glucose	-	?
3.2.1.B23	ginsenoside G75 + H2O	-	Thermus thermophilus	ginsenoside C-K + D-glucose	-	?
3.2.1.B23	ginsenoside Mc + H2O	-	Caldicellulosiruptor saccharolyticus	ginsenoside C-K + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Mc + H2O	-	Rhodanobacter ginsenosidimutans	ginsenoside C-K + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Mc1 + H2O	-	Rhodanobacter ginsenosidimutans	ginsenoside F2 + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Thermus thermophilus	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Trichoderma viride	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Aspergillus sp.	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Bifidobacterium longum	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Lissachatina fulica	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Fulvia fulva	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Pseudothermotoga thermarum	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Flavobacterium johnsoniae	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Bifidobacterium longum H-1	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Pseudothermotoga thermarum DSM 5069T	ginsenoside Rd + D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Aspergillus niger	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Pseudonocardia sp.	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.B23	ginsenoside Rb1 + H2O	-	Microbacterium esteraromaticum	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	-	Trichoderma viride	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	-	Bifidobacterium longum	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	-	Bifidobacterium choerinum	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rb2 + H2O	-	Microbacterium esteraromaticum	ginsenoside Rg3 + D-glucose + L-arabinopyranose	-	?
3.2.1.B23	ginsenoside Rb3 + H2O	-	Pseudonocardia sp.	ginsenoside Rg3 + D-glucose + D-xylose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Bifidobacterium breve	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Trichoderma viride	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Caldicellulosiruptor saccharolyticus	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Bifidobacterium longum	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Panax ginseng	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Leuconostoc sp.	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Rhodanobacter ginsenosidimutans	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rc + H2O	-	Bifidobacterium longum H-1	ginsenoside Rd + L-arabinofuranose	-	?
3.2.1.B23	ginsenoside Rd + H2O	-	Pseudonocardia sp.	ginsenoside Rg3 + D-glucose	-	?
3.2.1.B23	ginsenoside Re + H2O	-	Mucilaginibacter sp. QM49	ginsenoside Rg2 + D-glucose	-	?
3.2.1.B23	ginsenoside Rg1 + H2O	-	Mucilaginibacter sp. QM49	ginsenoside Rh1 + D-glucose	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Thermus thermophilus	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Trichoderma viride	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Aspergillus sp.	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Bifidobacterium longum	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Lissachatina fulica	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Fulvia fulva	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Pseudothermotoga thermarum	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Flavobacterium johnsoniae	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium breve	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Trichoderma viride	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Caldicellulosiruptor saccharolyticus	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium longum	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium choerinum	?	-	?
3.2.1.B23	additional information	hydrolysis of the C-20 inner Glc	Aspergillus niger	?	-	?
3.2.1.B23	additional information	hydrolysis of the C-20 inner Glc	Pseudonocardia sp.	?	-	?
3.2.1.B23	additional information	hydrolysis of the C-20 inner Glc	Rhizobium sp.	?	-	?
3.2.1.B23	additional information	hydrolysis of the C-20 inner Glc	Microbacterium esteraromaticum	?	-	?
3.2.1.B23	additional information	hydrolysis of the C-20 inner Glc	Mucilaginibacter sp. QM49	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Bifidobacterium longum H-1	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium longum H-1	?	-	?
3.2.1.B23	additional information	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)	Pseudothermotoga thermarum DSM 5069T	?	-	?
3.2.1.191	ginsenoside Rb1 + H2O	-	Sphingomonas sp.	ginsenoside G17 + D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	-	Sphingopyxis alaskensis	ginsenoside G17 + D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	via ginsenoside G17	Terrabacter ginsenosidimutans	ginsenoside G75 + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	via ginsenoside G17	Esteya vermicola	ginsenoside G75 + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rb1 + H2O	via ginsenoside G17	Esteya vermicola CNU120806	ginsenoside G75 + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rb2 + H2O	-	Sphingomonas sp.	ginsenoside C-O + D-glucose	-	?
3.2.1.191	ginsenoside Rb2 + H2O	-	Sphingopyxis alaskensis	ginsenoside C-O + D-glucose	-	?
3.2.1.191	ginsenoside Rb2 + H2O	via ginsenoside C-O	Terrabacter ginsenosidimutans	ginsenoside C-Y + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rb3 + H2O	via ginsenoside C-Mx1	Terrabacter ginsenosidimutans	ginsenoside C-Mx + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rc + H2O	-	Sphingomonas sp.	ginsenoside C-Mc1 + D-glucose	-	?
3.2.1.191	ginsenoside Rc + H2O	-	Sphingopyxis alaskensis	ginsenoside C-Mc1 + D-glucose	-	?
3.2.1.191	ginsenoside Rc + H2O	via ginsenoside C-Mc1	Terrabacter ginsenosidimutans	ginsenoside C-Mc + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	via ginsenoside F2	Esteya vermicola	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	via ginsenosside F2	Terrabacter ginsenosidimutans	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	via ginsenosside F2	Esteya vermicola	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	via ginsenoside F2	Esteya vermicola CNU120806	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	via ginsenosside F2	Esteya vermicola CNU120806	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	-	Sphingomonas sp.	ginsenoside F2 + D-glucose	-	?
3.2.1.191	ginsenoside Rd + H2O	-	Sphingopyxis alaskensis	ginsenoside F2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	-	Fusarium sp.	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	-	Fusarium proliferatum	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	-	Panax ginseng	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	-	Sphingopyxis alaskensis	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	-	Fusarium proliferatum ECU2042	ginsenoside Rh2 + D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	via ginsenoside Rh2	Terrabacter ginsenosidimutans	protopanaxadiol aglycone + 2 D-glucose	-	?
3.2.1.191	ginsenoside Rg3 + H2O	via ginsenoside Rh2, hydrolysis of the inner glucose at C-3	Aspergillus niger	protopanaxadiol aglycone + 2 D-glucose	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Aspergillus niger	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Fusarium sp.	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Sphingomonas sp.	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Fusarium proliferatum	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Panax ginseng	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Terrabacter ginsenosidimutans	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Sphingopyxis alaskensis	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Esteya vermicola	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Aspergillus niger	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Fusarium sp.	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Sphingomonas sp.	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Fusarium proliferatum	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Panax ginseng	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Terrabacter ginsenosidimutans	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Esteya vermicola	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides. beta-Glucosidase from Sphingopyxis alaskensis hydrolyzes only the outer glucose at C-3 in ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to product G17, C-O, C-Mc1, F2 and Rh2, respectively, without further hydrolysis	Sphingopyxis alaskensis	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Esteya vermicola CNU120806	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Esteya vermicola CNU120806	?	-	?
3.2.1.191	additional information	ginsenosidase type III are classified into types III-i and III-ii, which hydrolyze the outer glucose and inner glucose linked to C-3 in the PPD-type ginsenosides, respectively. Ginsenosidase type III-i converts ginsenosides Rb1, Rb2, Rc, Rd and Rg3 to G17, C-O, C-Mc1, F2 and Rh2, respectively, by hydrolyzing only the outer glucose linked to C-3 in the PPD-type ginsenosides	Fusarium proliferatum ECU2042	?	-	?
3.2.1.191	additional information	ginsenosidase type III-i catalyzes the hydrolysis of only C-3 outer glucose in PPD-type gindenosides. Ginsenosidase type III-ii catalyzes the hydrolysis of outer and inner, or only inner glucose in PPD-type ginsenosides	Fusarium proliferatum ECU2042	?	-	?
3.2.1.193	ginsenoside F2 + H2O	-	Sphingomonas leidyi	ginsenoside C-K + D-glucose	-	?
3.2.1.193	ginsenoside F2 + H2O	-	Aspergillus oryzae	ginsenoside C-K + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenoside F2	Saccharolobus solfataricus	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenoside F2	Aspergillus sp.	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenoside F2	Leuconostoc citreum	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Sulfolobus acidocaldarius	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Leuconostoc mesenteroides	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Lactiplantibacillus pentosus	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Microbacterium esteraromaticum	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Fusobacterium sp.	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Companilactobacillus paralimentarius	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Sphingomonas leidyi	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides Rd and F2	Aspergillus niger	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Lactiplantibacillus pentosus 6105	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides F2	Leuconostoc mesenteroides DC102	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenoside F2	Leuconostoc citreum LH1	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenosides Rd and F2	Aspergillus niger g.848	ginsenoside C-K + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Aspergillus oryzae	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Pseudarthrobacter chlorophenolicus	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Intrasporangium sp.	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Globisporangium irregulare	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Nocardioides panaciterrulae	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Sphingomonas leidyi	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Intrasporangium sp. GS603	ginsenoside F2 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Paecilomyces variotii	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Citrus limon	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	-	Paecilomyces variotii 229-7	ginsenoside Rg3 + 2 D-glucose	-	?
3.2.1.193	ginsenoside Rb1 + H2O	via ginsenoside F2	Aspergillus sp.	ginsenoside Rh2 + 3 D-glucose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	-	Aspergillus oryzae	ginsenoside F2 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	-	Pseudarthrobacter chlorophenolicus	ginsenoside F2 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	-	Globisporangium irregulare	ginsenoside F2 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	-	Sulfolobus acidocaldarius	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	-	Microbacterium esteraromaticum	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	via ginsenoside F2	Saccharolobus solfataricus	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	via ginsenoside F2	Aspergillus sp.	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	via ginsenosides Rd and F2	Aspergillus niger	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	via ginsenosides Rd and F2	Aspergillus niger g.848	ginsenoside C-K + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	-	Citrus limon	ginsenoside Rg3 + D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb2 + H2O	via ginsenoside F2	Aspergillus sp.	ginsenoside Rh2 + 2 D-glucose + L-arabinopyranose	-	?
3.2.1.193	ginsenoside Rb3 + H2O	via ginsenoside F2	Aspergillus niger	ginsenoside C-K + 2 D-glucose + xylose	-	?
3.2.1.193	ginsenoside Rb3 + H2O	via ginsenoside F2	Aspergillus niger g.848	ginsenoside C-K + 2 D-glucose + xylose	-	?
3.2.1.193	ginsenoside Rc + H2O	-	Aspergillus oryzae	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	-	Pseudarthrobacter chlorophenolicus	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	-	Globisporangium irregulare	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	-	Nocardioides panaciterrulae	ginsenoside F2 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	via ginsenoside F2	Aspergillus niger	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	via ginsenoside F2	Saccharolobus solfataricus	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	via ginsenoside F2	Aspergillus sp.	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	via ginsenoside F2	Aspergillus niger g.848	ginsenoside C-K + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	-	Citrus limon	ginsenoside Rg3 + D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rc + H2O	via ginsenoside F2	Aspergillus sp.	ginsenoside Rh2 + 2 D-glucose + L-arabinofuranose	-	?
3.2.1.193	ginsenoside Rd + H2O	-	Paecilomyces variotii	ginsenoside Rg3 + D-glucose	-	?
3.2.1.193	ginsenoside Rd + H2O	-	Paecilomyces variotii 229-7	ginsenoside Rg3 + D-glucose	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Aspergillus niger	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Paecilomyces variotii	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Sulfolobus acidocaldarius	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Leuconostoc mesenteroides	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Lactiplantibacillus pentosus	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Saccharolobus solfataricus	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Aspergillus oryzae	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Aspergillus sp.	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Microbacterium esteraromaticum	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Citrus limon	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Leuconostoc citreum	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Fusobacterium sp.	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Pseudarthrobacter chlorophenolicus	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Intrasporangium sp.	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Globisporangium irregulare	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Nocardioides panaciterrulae	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Companilactobacillus paralimentarius	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Sphingomonas leidyi	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Lactiplantibacillus pentosus 6105	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Intrasporangium sp. GS603	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Leuconostoc mesenteroides DC102	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Leuconostoc citreum LH1	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Aspergillus niger g.848	?	-	?
3.2.1.193	additional information	ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D	Paecilomyces variotii 229-7	?	-	?
3.2.1.194	ginsenoside R1 + H2O	-	Aspergillus sp.	ginsenoside F1 + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Re + H2O	-	Pyrococcus furiosus	ginsenoside Rg1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Re + H2O	-	Aspergillus sp.	ginsenoside F1 + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Re + H2O	-	Penicillium decumbens	ginsenoside F1 + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Rf + H2O	-	Aspergillus niger	ginsenoside Rh1 + D-glucose	-	?
3.2.1.194	ginsenoside Rf + H2O	-	Pyrococcus furiosus	ginsenoside Rh1 + D-glucose	-	?
3.2.1.194	ginsenoside Rf + H2O	-	Aspergillus sp.	protopanaxatriol + 2 D-glucose	-	?
3.2.1.194	ginsenoside Rf + H2O	-	Penicillium decumbens	protopanaxatriol + 2 D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	-	Penicillium decumbens	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	-	Fusarium subglutinans	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	-	Penicillium sclerotiorum	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg1 + H2O	-	Sanguibacter keddieii	ginsenoside F1 + D-glucose	-	?
3.2.1.194	ginsenoside Rg2 + 2 H2O	-	Penicillium decumbens	protopanaxatriol + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	-	Pyrococcus furiosus	ginsenoside Rh1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	-	Thermoanaerobacterium thermosaccharolyticum	ginsenoside Rh1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	-	Absidia sp. (in: Fungi)	ginsenoside Rh1 + L-rhamnose	-	?
3.2.1.194	ginsenoside Rg2 + H2O	-	Aspergillus sp.	protopanaxatriol + D-glucose + L-rhamnose	-	?
3.2.1.194	ginsenoside Rh1 + H2O	-	Penicillium decumbens	protopanaxatriol + D-glucose	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Aspergillus niger	Aspergillus niger	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	Absidia sp. (in: Fungi)	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion was performed using b-glycosidase from Pyrococcus furiosus	Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Thermoanaerobacterium thermosaccharolyticum	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium decumbens	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Fusarium subglutinans	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium sclerotiorum	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Sanguibacter keddieii	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3	Aspergillus sp.	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1. This conversion has been conducted by beta-glycosidase from Pyrococcus furiosus	Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2. This conversion is performed using beta-glycosidase from Pyrococcus furiosus	Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides. beta-Gglycosidase from Pyrococcus furiosus shows broad substrate specificity that hydrolyzes the outer residue glucose and rhamnose and xylose at C-6	Pyrococcus furiosus	?	-	?
3.2.1.194	additional information	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides. beta-Xylosidase from Thermoanaerobacterium thermosaccharolyticum converts notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1 by selectively hydrolyzing only the outer xylose at C-6	Thermoanaerobacterium thermosaccharolyticum	?	-	?
3.2.1.194	notoginsenoside R1 + H2O	-	Pyrococcus furiosus	ginsenoside Rg1 + D-xylose	-	?
3.2.1.194	notoginsenoside R1 + H2O	-	Thermoanaerobacterium thermosaccharolyticum	ginsenoside Rg1 + D-xylose	-	?
3.2.1.195	ginsenoside Re + H2O	-	Penicillium sp.	ginsenoside Rh1 + D-glucose + L-rhamnose	-	?
3.2.1.195	ginsenoside Re + H2O	-	Actinosynnema mirum	ginsenoside Rh1 + D-glucose + L-rhamnose	-	?
3.2.1.195	ginsenoside Rg1 + H2O	via ginsenoside Rh1	Penicillium sp.	protopanaxatriol + 2 D-glucose	-	?
3.2.1.195	ginsenoside Rg1 + H2O	via ginsenoside Rh1	Actinosynnema mirum	protopanaxatriol + 2 D-glucose	-	?
3.2.1.195	additional information	ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively	Penicillium sp.	?	-	?
3.2.1.195	additional information	ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively	Actinosynnema mirum	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.B23	?	x * 56000	Rhodanobacter ginsenosidimutans
3.2.1.B23	?	x * 57000	Bifidobacterium longum
3.2.1.B23	?	x * 57000	Pseudothermotoga thermarum
3.2.1.B23	?	x * 49000	Thermus thermophilus
3.2.1.B23	?	x * 58000	Caldicellulosiruptor saccharolyticus
3.2.1.B23	?	x * 58000	Leuconostoc sp.
3.2.1.B23	?	x * 80000	Caldicellulosiruptor saccharolyticus
3.2.1.B23	?	x * 80000	Fulvia fulva
3.2.1.B23	?	x * 88000	Microbacterium esteraromaticum
3.2.1.B23	?	x * 60000	Aspergillus sp.
3.2.1.B23	?	x * 86000	Panax ginseng
3.2.1.B23	?	x * 120000	Lissachatina fulica
3.2.1.B23	?	x * 77000	Bifidobacterium breve
3.2.1.B23	?	x * 79000	Pseudonocardia sp.
3.2.1.B23	?	x * 81000	Bifidobacterium longum
3.2.1.B23	?	x * 82000	Flavobacterium johnsoniae
3.2.1.B23	?	x * 85000	Mucilaginibacter sp. QM49
3.2.1.B23	?	x * 87000	Microbacterium esteraromaticum
3.2.1.B23	?	x * 90000	Fulvia fulva
3.2.1.B23	?	x * 95000	Bifidobacterium longum
3.2.1.191	?	x * 51000	Sphingopyxis alaskensis
3.2.1.191	?	x * 49000	Sphingomonas sp.
3.2.1.191	?	x * 70000	Terrabacter ginsenosidimutans
3.2.1.191	?	x * 59000	Panax ginseng
3.2.1.193	?	x * 45000	Leuconostoc citreum
3.2.1.193	?	x * 45000	Pseudarthrobacter chlorophenolicus
3.2.1.193	?	x * 57000	Sulfolobus acidocaldarius
3.2.1.193	?	x * 57000	Saccharolobus solfataricus
3.2.1.193	?	x * 12000, native enzyme	Paecilomyces variotii
3.2.1.193	?	x * 74000	Aspergillus niger
3.2.1.193	?	x * 79000	Microbacterium esteraromaticum
3.2.1.194	?	x * 40000	Penicillium sclerotiorum
3.2.1.194	?	x * 56000	Aspergillus sp.
3.2.1.194	?	x * 53000	Absidia sp. (in: Fungi)
3.2.1.194	?	x * 59000	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	?	x * 54000	Pyrococcus furiosus
3.2.1.194	?	x * 67000	Sanguibacter keddieii
3.2.1.194	?	x * 85000	Fusarium subglutinans
3.2.1.194	?	x * 93000	Aspergillus niger
3.2.1.195	?	x * 65000	Actinosynnema mirum

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.B23	alpha-L-arabinofuranosidase	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	alpha-L-arabinofuranosidase	-	Bifidobacterium longum
3.2.1.B23	alpha-L-arabinofuranosidase	-	Panax ginseng
3.2.1.B23	alpha-L-arabinofuranosidase	-	Leuconostoc sp.
3.2.1.B23	alpha-L-arabinofuranosidase	-	Rhodanobacter ginsenosidimutans
3.2.1.B23	alpha-L-arabinopyranosidase	-	Bifidobacterium breve
3.2.1.B23	alpha-L-arabinopyranosidase	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	alpha-L-arabinopyranosidase	-	Bifidobacterium longum
3.2.1.B23	beta-galactosidase	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	beta-glucosidase	-	Thermus thermophilus
3.2.1.B23	beta-glucosidase	-	Pseudonocardia sp.
3.2.1.B23	beta-glucosidase	-	Aspergillus sp.
3.2.1.B23	beta-glucosidase	-	Microbacterium esteraromaticum
3.2.1.B23	beta-glucosidase	-	Bifidobacterium longum
3.2.1.B23	beta-glucosidase	-	Lissachatina fulica
3.2.1.B23	beta-glucosidase	-	Pseudothermotoga thermarum
3.2.1.B23	beta-glucosidase	-	Flavobacterium johnsoniae
3.2.1.B23	beta-glucosidase	-	Mucilaginibacter sp. QM49
3.2.1.B23	beta-glucosidase GI	-	Fulvia fulva
3.2.1.B23	beta-glucosidase GII	-	Fulvia fulva
3.2.1.B23	ginsenosidase type II	-	Thermus thermophilus
3.2.1.B23	ginsenosidase type II	-	Aspergillus niger
3.2.1.B23	ginsenosidase type II	-	Pseudonocardia sp.
3.2.1.B23	ginsenosidase type II	-	Rhizobium sp.
3.2.1.B23	ginsenosidase type II	-	Bifidobacterium breve
3.2.1.B23	ginsenosidase type II	-	Trichoderma viride
3.2.1.B23	ginsenosidase type II	-	Aspergillus sp.
3.2.1.B23	ginsenosidase type II	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	ginsenosidase type II	-	Microbacterium esteraromaticum
3.2.1.B23	ginsenosidase type II	-	Bifidobacterium longum
3.2.1.B23	ginsenosidase type II	-	Lissachatina fulica
3.2.1.B23	ginsenosidase type II	-	Panax ginseng
3.2.1.B23	ginsenosidase type II	-	Fulvia fulva
3.2.1.B23	ginsenosidase type II	-	Leuconostoc sp.
3.2.1.B23	ginsenosidase type II	-	Pseudothermotoga thermarum
3.2.1.B23	ginsenosidase type II	-	Flavobacterium johnsoniae
3.2.1.B23	ginsenosidase type II	-	Bifidobacterium choerinum
3.2.1.B23	ginsenosidase type II	-	Rhodanobacter ginsenosidimutans
3.2.1.B23	ginsenosidase type II	-	Mucilaginibacter sp. QM49
3.2.1.B23	ginsenosidase type II-i-1	-	Thermus thermophilus
3.2.1.B23	ginsenosidase type II-i-1	-	Trichoderma viride
3.2.1.B23	ginsenosidase type II-i-1	-	Aspergillus sp.
3.2.1.B23	ginsenosidase type II-i-1	-	Bifidobacterium longum
3.2.1.B23	ginsenosidase type II-i-1	-	Lissachatina fulica
3.2.1.B23	ginsenosidase type II-i-1	-	Fulvia fulva
3.2.1.B23	ginsenosidase type II-i-1	-	Pseudothermotoga thermarum
3.2.1.B23	ginsenosidase type II-i-1	-	Flavobacterium johnsoniae
3.2.1.B23	ginsenosidase type II-i-2	-	Bifidobacterium breve
3.2.1.B23	ginsenosidase type II-i-2	-	Trichoderma viride
3.2.1.B23	ginsenosidase type II-i-2	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	ginsenosidase type II-i-2	-	Bifidobacterium longum
3.2.1.B23	ginsenosidase type II-i-2	-	Bifidobacterium choerinum
3.2.1.B23	ginsenosidase type II-i-4	-	Trichoderma viride
3.2.1.B23	ginsenosidase type II-i-4	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	ginsenosidase type II-i-4	-	Bifidobacterium longum
3.2.1.B23	ginsenosidase type II-i-4	-	Panax ginseng
3.2.1.B23	ginsenosidase type II-i-4	-	Leuconostoc sp.
3.2.1.B23	ginsenosidase type II-i-4	-	Rhodanobacter ginsenosidimutans
3.2.1.B23	ginsenosidase type II-ii	-	Aspergillus niger
3.2.1.B23	ginsenosidase type II-ii	-	Pseudonocardia sp.
3.2.1.B23	ginsenosidase type II-ii	-	Rhizobium sp.
3.2.1.B23	ginsenosidase type II-ii	-	Microbacterium esteraromaticum
3.2.1.B23	ginsenosidase type II-ii	-	Mucilaginibacter sp. QM49
3.2.1.191	beta-glucosidase	-	Sphingomonas sp.
3.2.1.191	beta-glucosidase	-	Panax ginseng
3.2.1.191	beta-glucosidase	-	Sphingopyxis alaskensis
3.2.1.191	beta-glucosidase	-	Esteya vermicola
3.2.1.191	ginsenosidase type III-ii	-	Aspergillus niger
3.2.1.191	ginsenosidase type III-ii	-	Fusarium sp.
3.2.1.191	ginsenosidase type III-ii	-	Sphingomonas sp.
3.2.1.191	ginsenosidase type III-ii	-	Fusarium proliferatum
3.2.1.191	ginsenosidase type III-ii	-	Panax ginseng
3.2.1.191	ginsenosidase type III-ii	-	Terrabacter ginsenosidimutans
3.2.1.191	ginsenosidase type III-ii	-	Sphingopyxis alaskensis
3.2.1.191	ginsenosidase type III-ii	-	Esteya vermicola
3.2.1.193	beta-glucosidase	-	Paecilomyces variotii
3.2.1.193	beta-glucosidase	-	Sulfolobus acidocaldarius
3.2.1.193	beta-glucosidase	-	Saccharolobus solfataricus
3.2.1.193	beta-glucosidase	-	Aspergillus sp.
3.2.1.193	beta-glucosidase	-	Microbacterium esteraromaticum
3.2.1.193	beta-glucosidase	-	Leuconostoc citreum
3.2.1.193	beta-glucosidase	-	Fusobacterium sp.
3.2.1.193	beta-glucosidase	-	Pseudarthrobacter chlorophenolicus
3.2.1.193	ginsenosidase type I	-	Aspergillus niger
3.2.1.193	ginsenosidase type I	-	Paecilomyces variotii
3.2.1.193	ginsenosidase type I	-	Sulfolobus acidocaldarius
3.2.1.193	ginsenosidase type I	-	Leuconostoc mesenteroides
3.2.1.193	ginsenosidase type I	-	Lactiplantibacillus pentosus
3.2.1.193	ginsenosidase type I	-	Saccharolobus solfataricus
3.2.1.193	ginsenosidase type I	-	Aspergillus oryzae
3.2.1.193	ginsenosidase type I	-	Aspergillus sp.
3.2.1.193	ginsenosidase type I	-	Microbacterium esteraromaticum
3.2.1.193	ginsenosidase type I	-	Citrus limon
3.2.1.193	ginsenosidase type I	-	Leuconostoc citreum
3.2.1.193	ginsenosidase type I	-	Fusobacterium sp.
3.2.1.193	ginsenosidase type I	-	Pseudarthrobacter chlorophenolicus
3.2.1.193	ginsenosidase type I	-	Intrasporangium sp.
3.2.1.193	ginsenosidase type I	-	Globisporangium irregulare
3.2.1.193	ginsenosidase type I	-	Nocardioides panaciterrulae
3.2.1.193	ginsenosidase type I	-	Companilactobacillus paralimentarius
3.2.1.193	ginsenosidase type I	-	Sphingomonas leidyi
3.2.1.193	ginsenosidase type-I	-	Aspergillus niger
3.2.1.194	alpha-L-rhamnosidase	-	Absidia sp. (in: Fungi)
3.2.1.194	beta-glucosidase	-	Pyrococcus furiosus
3.2.1.194	beta-glucosidase	-	Fusarium subglutinans
3.2.1.194	beta-glucosidase	-	Penicillium sclerotiorum
3.2.1.194	beta-glucosidase	-	Sanguibacter keddieii
3.2.1.194	beta-glycosidase	-	Aspergillus niger
3.2.1.194	beta-xylosidase	-	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	ginsenosidase type IV	-	Aspergillus sp.
3.2.1.194	ginsenosidase type IV-i-1	-	Aspergillus niger
3.2.1.194	ginsenosidase type IV-i-1	-	Pyrococcus furiosus
3.2.1.194	ginsenosidase type IV-i-2	-	Pyrococcus furiosus
3.2.1.194	ginsenosidase type IV-i-2	-	Absidia sp. (in: Fungi)
3.2.1.194	ginsenosidase type IV-i-3	-	Pyrococcus furiosus
3.2.1.194	ginsenosidase type IV-i-3	-	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	ginsenosidase type IV-ii	-	Aspergillus sp.
3.2.1.194	ginsenosidase type IV-ii	-	Penicillium decumbens
3.2.1.194	ginsenosidase type IV-ii	-	Fusarium subglutinans
3.2.1.194	ginsenosidase type IV-ii	-	Penicillium sclerotiorum
3.2.1.194	ginsenosidase type IV-ii	-	Sanguibacter keddieii
3.2.1.195	beta-glucosidase	-	Penicillium sp.
3.2.1.195	beta-glucosidase	-	Actinosynnema mirum
3.2.1.195	ginsenosidase type V	-	Penicillium sp.
3.2.1.195	ginsenosidase type V	-	Actinosynnema mirum

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.2.1.B23	30	-	-	Mucilaginibacter sp. QM49
3.2.1.B23	35	-	-	Bifidobacterium longum
3.2.1.B23	37	-	-	Pseudonocardia sp.
3.2.1.B23	37	-	-	Bifidobacterium breve
3.2.1.B23	37	-	-	Bifidobacterium longum
3.2.1.B23	37	-	-	Leuconostoc sp.
3.2.1.B23	37	-	-	Flavobacterium johnsoniae
3.2.1.B23	37	-	-	Rhodanobacter ginsenosidimutans
3.2.1.B23	40	-	-	Microbacterium esteraromaticum
3.2.1.B23	45	-	-	Fulvia fulva
3.2.1.B23	50	-	-	Aspergillus sp.
3.2.1.B23	50	-	-	Panax ginseng
3.2.1.B23	70	-	-	Lissachatina fulica
3.2.1.B23	80	-	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	90	-	-	Thermus thermophilus
3.2.1.B23	90	-	-	Pseudothermotoga thermarum
3.2.1.191	37	-	-	Sphingomonas sp.
3.2.1.191	37	-	-	Terrabacter ginsenosidimutans
3.2.1.191	40	-	-	Sphingopyxis alaskensis
3.2.1.191	50	-	-	Esteya vermicola
3.2.1.191	60	-	-	Panax ginseng
3.2.1.193	37	-	-	Leuconostoc citreum
3.2.1.193	37	-	-	Pseudarthrobacter chlorophenolicus
3.2.1.193	40	-	-	Microbacterium esteraromaticum
3.2.1.193	45	-	-	Aspergillus niger
3.2.1.193	60	-	-	Paecilomyces variotii
3.2.1.193	85	-	-	Sulfolobus acidocaldarius
3.2.1.193	90	-	-	Saccharolobus solfataricus
3.2.1.194	25	-	-	Sanguibacter keddieii
3.2.1.194	37	-	-	Aspergillus niger
3.2.1.194	40	-	-	Aspergillus sp.
3.2.1.194	40	-	-	Absidia sp. (in: Fungi)
3.2.1.194	50	-	-	Fusarium subglutinans
3.2.1.194	60	-	-	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	65	-	-	Penicillium sclerotiorum
3.2.1.194	95	-	-	Pyrococcus furiosus
3.2.1.195	37	-	-	Actinosynnema mirum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.2.1.B23	4.7	-	-	Bifidobacterium longum
3.2.1.B23	4.8	-	-	Pseudothermotoga thermarum
3.2.1.B23	5	-	-	Aspergillus sp.
3.2.1.B23	5	-	-	Panax ginseng
3.2.1.B23	5.5	-	-	Bifidobacterium breve
3.2.1.B23	5.5	-	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	5.5	-	-	Bifidobacterium longum
3.2.1.B23	5.5	-	-	Fulvia fulva
3.2.1.B23	5.6	-	-	Lissachatina fulica
3.2.1.B23	6	-	-	Caldicellulosiruptor saccharolyticus
3.2.1.B23	6	-	-	Fulvia fulva
3.2.1.B23	6	-	-	Leuconostoc sp.
3.2.1.B23	6	-	-	Flavobacterium johnsoniae
3.2.1.B23	6.5	-	-	Thermus thermophilus
3.2.1.B23	6.8	-	-	Bifidobacterium longum
3.2.1.B23	7	-	-	Pseudonocardia sp.
3.2.1.B23	7	-	-	Microbacterium esteraromaticum
3.2.1.B23	7.5	-	-	Rhodanobacter ginsenosidimutans
3.2.1.B23	8	-	-	Mucilaginibacter sp. QM49
3.2.1.191	5	-	-	Sphingomonas sp.
3.2.1.191	5	-	-	Panax ginseng
3.2.1.191	5	-	-	Esteya vermicola
3.2.1.191	5.5	-	-	Sphingopyxis alaskensis
3.2.1.191	7	-	-	Terrabacter ginsenosidimutans
3.2.1.193	3.5	-	-	Paecilomyces variotii
3.2.1.193	5	-	-	Aspergillus niger
3.2.1.193	5.5	-	-	Sulfolobus acidocaldarius
3.2.1.193	5.5	-	-	Saccharolobus solfataricus
3.2.1.193	6	-	-	Leuconostoc citreum
3.2.1.193	6	-	-	Pseudarthrobacter chlorophenolicus
3.2.1.193	7	-	-	Microbacterium esteraromaticum
3.2.1.194	4.5	-	-	Penicillium sclerotiorum
3.2.1.194	5	-	-	Absidia sp. (in: Fungi)
3.2.1.194	5.5	-	-	Pyrococcus furiosus
3.2.1.194	6	-	-	Aspergillus sp.
3.2.1.194	6	-	-	Fusarium subglutinans
3.2.1.194	6.5	-	-	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	7.5	-	-	Aspergillus niger
3.2.1.194	8	-	-	Sanguibacter keddieii
3.2.1.195	7	-	-	Actinosynnema mirum

General Information

EC Number	General Information	Comment	Organism
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Thermus thermophilus
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Pseudothermotoga thermarum
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 2, GH2	Microbacterium esteraromaticum
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Pseudonocardia sp.
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Microbacterium esteraromaticum
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Bifidobacterium longum
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Flavobacterium johnsoniae
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Mucilaginibacter sp. QM49
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 42, GH42	Caldicellulosiruptor saccharolyticus
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 51, GH51	Caldicellulosiruptor saccharolyticus
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 51, GH51	Bifidobacterium longum
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 51, GH51	Leuconostoc sp.
3.2.1.B23	evolution	the enzyme belongs to the glycosyl hydrolase family 51, GH51	Rhodanobacter ginsenosidimutans
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2	Trichoderma viride
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2	Caldicellulosiruptor saccharolyticus
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2	Bifidobacterium longum
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2	Panax ginseng
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2	Leuconostoc sp.
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2	Rhodanobacter ginsenosidimutans
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium breve
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Trichoderma viride
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Caldicellulosiruptor saccharolyticus
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium longum
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)	Bifidobacterium choerinum
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Thermus thermophilus
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Trichoderma viride
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Aspergillus sp.
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Bifidobacterium longum
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Lissachatina fulica
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Fulvia fulva
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Pseudothermotoga thermarum
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns	Flavobacterium johnsoniae
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides	Aspergillus niger
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides	Pseudonocardia sp.
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides	Rhizobium sp.
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides	Microbacterium esteraromaticum
3.2.1.B23	physiological function	ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides	Mucilaginibacter sp. QM49
3.2.1.191	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Sphingomonas sp.
3.2.1.191	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Sphingopyxis alaskensis
3.2.1.191	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Aspergillus niger
3.2.1.191	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Fusarium sp.
3.2.1.191	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Terrabacter ginsenosidimutans
3.2.1.191	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Esteya vermicola
3.2.1.193	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Sulfolobus acidocaldarius
3.2.1.193	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Saccharolobus solfataricus
3.2.1.193	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Leuconostoc citreum
3.2.1.193	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Pseudarthrobacter chlorophenolicus
3.2.1.193	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Microbacterium esteraromaticum
3.2.1.194	evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Pyrococcus furiosus
3.2.1.194	evolution	the enzyme belongs to the glycosyl hydrolase family 39, GH39	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Aspergillus niger
3.2.1.194	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Aspergillus sp.
3.2.1.194	evolution	the enzyme belongs to the glycosyl hydrolase family 3, GH3	Sanguibacter keddieii
3.2.1.194	evolution	the enzyme belongs to the glycosyl hydrolase family 78, GH78	Absidia sp. (in: Fungi)
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	Aspergillus niger
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-1 enzymes convert ginsenoside Rf to Rh1 by the hydrolysis of only the outer glucose linked to C-20 and not the inner glucose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-1	Pyrococcus furiosus
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	Pyrococcus furiosus
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-2 enzymes convert ginsenosides Re and Rg2 to ginsenosides Rg1 and Rh1, respectively, by hydrolyzing only the outer rhamnose linked to C-6 in the PPT-type ginsenosides with the hydrolysis pattern of E-2	Absidia sp. (in: Fungi)
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Pyrococcus furiosus
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Thermoanaerobacterium thermosaccharolyticum
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium decumbens
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Fusarium subglutinans
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Penicillium sclerotiorum
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-i-3 enzymes convert notoginsenosides R1 and R2 to ginsenosides Rg1 and Rh1, respectively, with the hydrolysis pattern of E-3 through the hydrolysis of the outer xylose linked to C-6 without further hydrolysis in the PPT-type ginsenosides	Sanguibacter keddieii
3.2.1.194	physiological function	ginsenosidase type IV hydrolyzes the sugars linked to C-6 in the PPT-type ginsenosides. Ginsenosidase type IV enzymes are subdivided into types IV-i-1, IV-i-2, IV-i-3, and IV-ii. Ginsenosidase type IV-ii enzymes convert ginsenosides Rg1 and Rh1, containing only the inner glucose without the outer sugar at C-6, are converted to ginsenosides F1 and aglycone protopanaxatriol (APPT), respectively, by hydrolyzing the inner glucose linked to C-6 but not the glucose linked to C-20 in the PPT-type ginsenosides with the hydrolysis pattern of F. Ginsenosidase type IV-ii enzymes also hydrolyze the outer glucose of ginsenoside Rf, which has the outer and inner glucose at C-6 with the hydrolysis pattern of E-1; the outer rhamnose of ginsenosides Re and Rg2, which have the outer rhamnose and inner glucose at C-6 with the hydrolysis attern of E-2, and the outer xylose of notoginsenosides R1 and R2, which contain the outer xylose and inner glucose at C-6 with the hydrolysis pattern of E-3	Aspergillus sp.