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Literature summary extracted from

  • Varela-Ramirez, A.; Abendroth, J.; Mejia, A.A.; Phan, I.Q.; Lorimer, D.D.; Edwards, T.E.; Aguilera, R.J.
    Structure of acid deoxyribonuclease (2017), Nucleic Acids Res., 45, 6217-6227 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.22.1 expression in Escherichia coli Burkholderia thailandensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.22.1 crystals of BthDNase II grown from a solution containing 0.0004 ml of protein at 26.5 mg/ml and 0.0004 ml of precipitant equilibrated against 0.080 ml of precipitant (100 mM BisTris pH 6.5, 28% (w/v) PEG MME 2000). A single crystal is cryo-protected with 15% (v/v) ethylene glycol and vitrified in liquid nitrogen. Crystal structure solved at 1.65 A resolution Burkholderia thailandensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.22.1 Cu2+ inhibited by copper ions and to a lesser extent manganese ions, but not inhibited by calcium, magnesium or zinc ions Burkholderia thailandensis
3.1.22.1 Mn2+ inhibited by copper ions and to a lesser extent manganese ions, but not inhibited by calcium, magnesium or zinc ions Burkholderia thailandensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.22.1 40301
-
 calculated from sequence Burkholderia thailandensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Burkholderia thailandensis Q2T8B0
-
-
3.1.22.1 Burkholderia thailandensis ATCC 700388 Q2T8B0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.22.1
-
 Burkholderia thailandensis

Subunits

EC Number Subunits Comment Organism
3.1.22.1 dimer
-
 Burkholderia thailandensis

Synonyms

EC Number Synonyms Comment Organism
3.1.22.1 DNase II
-
 Burkholderia thailandensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.22.1 Burkholderia thailandensis calculated from sequence
-
 7