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Literature summary extracted from

  • Zeidler, S.; Hubloher, J.; Koenig, P.; Ngu, N.; Scholz, A.; Averhoff, B.; Mueller, V.
    Salt induction and activation of MtlD, the key enzyme in the synthesis of the compatible solute mannitol in Acinetobacter baumannii (2018), MicrobiologyOpen, 7, e00614 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.17 Na2SO4 at 1 M, 35% of maximum activity obtained with NaCl Acinetobacter baumannii
1.1.1.17 NaNO3 at 0.6 M, 25% of maximum activity obtained with NaCl Acinetobacter baumannii
1.1.1.17 sodium gluconate at 1.5 M, 135% of maximum activity obtained with NaCl Acinetobacter baumannii
1.1.1.17 sodium glutamate highest stimulations up to 41 U/mg at 0.8-1.2 M Acinetobacter baumannii
3.1.3.22 sodium glutamate highest stimulation up to 41 U/mg protein by sodium glutamate Acinetobacter baumannii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 expression in Escherichia coli Acinetobacter baumannii
3.1.3.22 expression in Escherichia coli Acinetobacter baumannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 KCl at 1.5 M, 52% of maximum activity obtained with NaCl Acinetobacter baumannii
1.1.1.17 NaCl activity is strictly salt dependent, with highest stimulation at 600 mM NaCl and 24% of maximum activity at 1.5 M Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 55
-
 D-fructose 6-phosphate pH 7.0, 37°C Acinetobacter baumannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.17 NaCl activity is strictly salt dependent, with highest stimulation at 600 mM NaCl Acinetobacter baumannii
3.1.3.22 NaCl enzyme activity is strictly salt dependent. Highest stimulation is reached at 600 mM NaCl Acinetobacter baumannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 87000
-
 gel filtration Acinetobacter baumannii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Acinetobacter baumannii D0C7J2
-
-
1.1.1.17 Acinetobacter baumannii ATCC 19606 D0C7J2
-
-
3.1.3.22 Acinetobacter baumannii D0C7J2 bifunctional mannitol-1-phosphate dehydrogenase/phosphatase
-
3.1.3.22 Acinetobacter baumannii ATCC 19606 D0C7J2 bifunctional mannitol-1-phosphate dehydrogenase/phosphatase
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 20
-
 presence of 600 mM NaCl, pH 7, 37°C Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-fructose 6-phosphate + NADH + H+
-
 Acinetobacter baumannii D-mannitol 1-phosphate + NAD+
-
 ?
1.1.1.17 D-fructose 6-phosphate + NADH + H+
-
 Acinetobacter baumannii ATCC 19606 D-mannitol 1-phosphate + NAD+
-
 ?
1.1.1.17 D-fructose 6-phosphate + NADPH + H+
-
 Acinetobacter baumannii D-mannitol 1-phosphate + NADP+
-
 ?
1.1.1.17 D-fructose 6-phosphate + NADPH + H+
-
 Acinetobacter baumannii ATCC 19606 D-mannitol 1-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.17 monomer 1 * 82000, SDS-PAGE recombinant His-tagged protein Acinetobacter baumannii
3.1.3.22 ? x * 82000, calculated from sequence Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 HAD hydrolase, family IA, variant 3
-
 Acinetobacter baumannii
1.1.1.17 HMPREF0010_00722
-
 Acinetobacter baumannii
1.1.1.17 MtlD
-
 Acinetobacter baumannii
3.1.3.22 HMPREF0010_00722
-
 Acinetobacter baumannii
3.1.3.22 MtlD
-
 Acinetobacter baumannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 37
-
-
 Acinetobacter baumannii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.17 20
-
 40% of the activity at 37°C Acinetobacter baumannii
1.1.1.17 45
-
 40% of the activity at 37°C Acinetobacter baumannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 7
-
-
 Acinetobacter baumannii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.17 5
-
 12% of maximum activity Acinetobacter baumannii
1.1.1.17 10
-
 12% of maximum activity Acinetobacter baumannii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NADH 5fold decrease in activity compared with NADPH Acinetobacter baumannii
1.1.1.17 NADPH NADPH is preferred over NADH Acinetobacter baumannii

Expression

EC Number Organism Comment Expression
1.1.1.17 Acinetobacter baumannii the presence of glycine betaine or choline repress promoter activation down
1.1.1.17 Acinetobacter baumannii expression of MtlD is strongly dependent on high osmolarity, not discriminating between different salts or sugars up
3.1.3.22 Acinetobacter baumannii expression of mtlD is strongly dependent on high osmolarity, not discriminating between different salts or sugars. The presence of glycine betaine or choline represses promoter activation additional information

General Information

EC Number General Information Comment Organism
1.1.1.17 physiological function after deletion of the mtlD gene, cells no longer accumulate mannitol and growth is completely impaired at high salt concentrations Acinetobacter baumannii
3.1.3.22 physiological function after deletion of the mtlD gene, cells no longer accumulate mannitol and growth is completely impaired at high salt concentrations Acinetobacter baumannii