Literature summary extracted from

Jiang, G.Z.; Yao, M.D.; Wang, Y.; Zhou, L.; Song, T.Q.; Liu, H.; Xiao, W.H.; Yuan, Y.J.
Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae (2017), Metab. Eng., 41, 57-66 .

Application

EC Number	Application	Comment	Organism
3.1.7.11	synthesis	synthesis of geraniol by expression of GES truncated at residue S43 in Saccharomyces cerevisiae. Coexpression of the reverse fusion of farnesyl diphosphate synthase Erg20 mutant F96W-N127W and truncated GES and another copy of farnesyl diphosphate synthase Erg20 mutant F96W-N127W promotes the geraniol titer to 523.96 mg/l at shakes flask level. A highest reported titer of 1.68 g/l geraniol in eukaryote cells is achieved in 2.0 l fed-batch fermentation under carbon restriction strategy	Catharanthus roseus

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.7.11	expression in Saccharomyces cerevisiae	Catharanthus roseus

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.7.11	D501A	30% of wild-type activity	Catharanthus roseus
3.1.7.11	additional information	N-terminal truncation at residues S14, L28, S43and S52 all lead to an increase on geraniol production	Catharanthus roseus
3.1.7.11	Y436A	12% of wild-type activity	Catharanthus roseus
3.1.7.11	Y436A/D501A	10% of wild-type activity	Catharanthus roseus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.1.7.11	plastid	-	Catharanthus roseus	9536	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.7.11	Catharanthus roseus	J9PZR5	-	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)