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Literature summary extracted from

  • Ishikawa, M.; Shiono, Y.; Koseki, T.
    Biochemical characterization of Aspergillus oryzae recombinant alpha-L-rhamnosidase expressed in Pichia pastoris (2017), J. Biosci. Bioeng., 124, 630-634 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expressed in Pichia pastoris AorhaA Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.39
-
 rutin pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 0.53
-
 naringin pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 1.4
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 1.71
-
 hesperidin pH 5.0, 50°C Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.40 glycoprotein
-
 Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 hesperidin + H2O 134% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside Aspergillus oryzae hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O 26% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside Aspergillus oryzae beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O 68% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside Aspergillus oryzae isoquercitrin + alpha-L-rhamnose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 AorhaA
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 70
-
-
 Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 45 75 45°C: about 60% of maximal activity, 75°C: about 45% of maximal activity Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 60
-
 1 h, enzyme retained more than 90% of its initial activity. It retains more than 80% of its initial activity after incubation for 2 h and does not further lose activity after treatment for 24 h Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.40 210
-
 naringin pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 550
-
 rutin pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 1080
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 2810
-
 hesperidin pH 5.0, 50°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 5
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 3 8 pH 3.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.40 400
-
 naringin pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 780
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 1410
-
 rutin pH 5.0, 50°C Aspergillus oryzae
3.2.1.40 1640
-
 hesperidin pH 5.0, 50°C Aspergillus oryzae