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Literature summary extracted from

  • Ikezawa, N.; Goepfert, J.C.; Nguyen, D.T.; Kim, S.U.; OMaille, P.E.; Spring, O.; Ro, D.K.
    Lettuce costunolide synthase (CYP71BL2) and its homolog (CYP71BL1) from sunflower catalyze distinct regio- and stereoselective hydroxylations in sesquiterpene lactone metabolism (2011), J. Biol. Chem., 286, 21601-21611 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.168 sunflower HaG8H and LsCOS are coexpressed with Artemisia annua CPR in pESCUra vector Helianthus annuus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Helianthus annuus
-
 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Helianthus annuus cv. HA300
-
 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.168 Helianthus annuus F8S1H3
-
-
1.14.14.168 Helianthus annuus cv. HA300 F8S1H3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
 Helianthus annuus 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid Helianthus annuus 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
 Helianthus annuus cv. HA300 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid Helianthus annuus cv. HA300 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.168 CYP71BL1
-
 Helianthus annuus
1.14.14.168 GAA 8x03-hydroxylase
-
 Helianthus annuus
1.14.14.168 HaG8H
-
 Helianthus annuus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.168 cytochrome P-450 heme-thiolate protein Helianthus annuus

General Information

EC Number General Information Comment Organism
1.14.14.168 evolution HaG8H appears to be restricted to the Heliantheae and possibly some other tribes in the Asteroideae subfamily Helianthus annuus