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Literature summary extracted from
- Structural characterization, catalytic, kinetic and thermodynamic properties of Aspergillus oryzae tannase (2016), Int. J. Biol. Macromol., 92, 803-811 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Co2+ | 25% inhibition at 10 mM | Aspergillus oryzae |  |
| 3.1.1.20 | Cu2+ | 53% inhibition at 10 mM | Aspergillus oryzae | |
| 3.1.1.20 | Fe2+ | 55% inhibition at 10 mM | Aspergillus oryzae | |
| 3.1.1.20 | Hg2+ | 73% inhibition at 10 mM | Aspergillus oryzae | |
| 3.1.1.20 | Zn2+ | 11% inhibition at 10 mM | Aspergillus oryzae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | additional information | - |
additional information | kinetics and thermodynamics analysis, temperature dependence of kinetic parameters on tannic acid hydrolysis, overview | Aspergillus oryzae | |
| 3.1.1.20 | 4.13 | - |
Tannic acid | pH 5.0, 37°C | Aspergillus oryzae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.20 | intracellular | - |
Aspergillus oryzae | 5622 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Ca2+ | activates 32% at 10 mM | Aspergillus oryzae | |
| 3.1.1.20 | additional information | poorly activating effect by Mg2+ at 10 mM | Aspergillus oryzae |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 106000 | - |
gel filtration and native PAGE | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | tannic acid + H2O | Aspergillus oryzae | - |
gallic acid + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Aspergillus oryzae RIB 40 | - |
gallic acid + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Aspergillus oryzae ATCC 42149 | - |
gallic acid + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Aspergillus oryzae | P78581 | from China green tea paste | - |
| 3.1.1.20 | Aspergillus oryzae ATCC 42149 | P78581 | from China green tea paste | - |
| 3.1.1.20 | Aspergillus oryzae RIB 40 | P78581 | from China green tea paste | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | native intracellular enzyme 36.7fold from submerged culture by ammonium sulfate fractionation, dialysis and desalting gel filtration, and two steps of gel filtration | Aspergillus oryzae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | mycelium | submerged growth conditions | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 2453.9 | - |
purified enzyme, pH 5.0, 37°C, substrate tannic acid | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | additional information | tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate | Aspergillus oryzae | ? | - |
? | |
| 3.1.1.20 | additional information | tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate | Aspergillus oryzae RIB 40 | ? | - |
? | |
| 3.1.1.20 | additional information | tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate | Aspergillus oryzae ATCC 42149 | ? | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Aspergillus oryzae | gallic acid + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Aspergillus oryzae RIB 40 | gallic acid + D-glucose | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Aspergillus oryzae ATCC 42149 | gallic acid + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | monomer | x * 106000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | TanA | - |
Aspergillus oryzae |
| 3.1.1.20 | tannin acyl hydrolase | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 37 | - |
- |
Aspergillus oryzae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | additional information | - |
temperature dependence of kinetic parameters on tannic acid hydrolysis, overview | Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 40 | 70 | purified native enzyme, the calculated half-life times at 40, 45, 50, 55, 60, and 70°C are 955.15, 142.0, 30.28, 17.88, 8.23, and 2.95 min, respectively, determination of thermodynamic parameters for irreversible thermal inactivation at 40-70°C | Aspergillus oryzae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 551.4 | - |
Tannic acid | pH 5.0, 37°C | Aspergillus oryzae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 5.5 | - |
- |
Aspergillus oryzae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 3 | 8 | activity range, profile overview | Aspergillus oryzae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 3.5 | 8.5 | purified native enzyme, 25°C, 180 min, over 50% activity remaining, completely stable at pH 4.5-7.0 | Aspergillus oryzae |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | physiological function | tannase catalyses the breakdown of ester and depside link-ages in hydrolysable tannins such as tannic acid, producing gallic acid and glucose | Aspergillus oryzae |
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