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Literature summary extracted from
- Characterization of an extremely salt-tolerant and thermostable phytase from Bacillus amyloliquefaciens US573 (2015), Int. J. Biol. Macromol., 80, 581-587 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | gene phy, DNA and amino acid sequence determination, analysis, and comparisons, cloning and expression in Escherichia coli strain DH5alpha | Bacillus amyloliquefaciens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | LiCl | enzyme PHY US573 is extremely salt-tolerant preserving 80% and 95% of activity in the presence of 20 g/l of NaCl and LiCl, respectively | Bacillus amyloliquefaciens |  |
| 3.1.3.8 | additional information | the enzyme is resistant to bovine bile extract | Bacillus amyloliquefaciens | |
| 3.1.3.8 | NaCl | enzyme PHY US573 is extremely salt-tolerant preserving 80% and 95% of activity in the presence of 20 g/l of NaCl and LiCl, respectively | Bacillus amyloliquefaciens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 1.125 | - |
myo-inositol hexakisphosphate | pH 7.5, 70°C | Bacillus amyloliquefaciens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.8 | extracellular | the enzyme is secreted | Bacillus amyloliquefaciens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | dependent on, best at 1 mM | Bacillus amyloliquefaciens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Bacillus amyloliquefaciens | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Bacillus amyloliquefaciens US573 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Bacillus amyloliquefaciens | A0A0G4QKE4 | isolated from geother-mal soil located in Southern Tunisia | - |
| 3.1.3.8 | Bacillus amyloliquefaciens US573 | A0A0G4QKE4 | isolated from geother-mal soil located in Southern Tunisia | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | native extracellular enzyme 6fold from cell culture medium of strain US573 by heat treatment and ammonium sulfate fractionation | Bacillus amyloliquefaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 27 | - |
native extracellular enzyme, pH 7.5, 70°C | Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | additional information | the enzyme is also able to dephosphorylate ATP and ADP with 95 and 29% of the activity compared to phytic acid, respectively | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.1.3.8 | additional information | the enzyme is also able to dephosphorylate ATP and ADP with 95 and 29% of the activity compared to phytic acid, respectively | Bacillus amyloliquefaciens US573 | ? | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus amyloliquefaciens | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | phytic acid | Bacillus amyloliquefaciens | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus amyloliquefaciens US573 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | phytic acid | Bacillus amyloliquefaciens US573 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | PHY US573 | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 70 | - |
- |
Bacillus amyloliquefaciens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 35 | 85 | activity range, profile overview | Bacillus amyloliquefaciens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 90 | 100 | 50% and 62% activity remaining after 10 min at 100°C and 90°C, respectively | Bacillus amyloliquefaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 7.5 | - |
- |
Bacillus amyloliquefaciens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 4.5 | 8.5 | activity range, profile overview | Bacillus amyloliquefaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 3 | 9 | purified enzyme, 37°C, shows good stability | Bacillus amyloliquefaciens |
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