EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.8 | F376Y | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | K432R | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | additional information | construction of three recombinant phyA mutant strains, i.e. PP-NPm-8, PP-NPep-6A and I44E/T252RPhyA, that show improved catalytic efficiency or thermostability compared to the wild-type strain. Variations of bond and electrostatic energy before and after substitution at Q172, T195, Q368, F376, and K432, half-life of thermal inactivation and kinetic parameters of PP-NPep-6A phytase and its mutants, predictions of residue interactions, overview | Aspergillus niger |
3.1.3.8 | Q172R | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | Q172R/K432R | site-directed mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | Q368E | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | Q368E/K432R | site-directed mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | T195L | random mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
3.1.3.8 | T195L/Q368E/F376Y | site-directed mutagenesis, the mutant has an altered thermostability compared to wild-type | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.01073 | - |
myo-inositol hexakisphosphate | recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.01547 | - |
myo-inositol hexakisphosphate | recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.01794 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.01927 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.02075 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.02189 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.02769 | - |
myo-inositol hexakisphosphate | recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.03073 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Aspergillus niger | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Aspergillus niger N25 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Aspergillus niger | P34754 | - |
- |
3.1.3.8 | Aspergillus niger N25 | P34754 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 286923 | - |
recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 303715 | - |
recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 438366 | - |
recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 466688 | - |
recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 467242 | - |
recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 478761 | - |
recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 493308 | - |
recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger |
3.1.3.8 | 503217 | - |
recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger N25 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | 3-phytase B | - |
Aspergillus niger |
3.1.3.8 | Aspergillus niger N25 phytase | - |
Aspergillus niger |
3.1.3.8 | PhyB | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 60 | - |
- |
Aspergillus niger |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 20 | 80 | activity range, profile overview | Aspergillus niger |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.422 | - |
myo-inositol hexakisphosphate | recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.476 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.496 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.548 | - |
myo-inositol hexakisphosphate | recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.553 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.557 | - |
myo-inositol hexakisphosphate | recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.576 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 0.71 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5.5 | - |
- |
Aspergillus niger |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 2 | 7.5 | activity range, profile overview | Aspergillus niger |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 16.14 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 20.08 | - |
myo-inositol hexakisphosphate | recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 24.7 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 25.26 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 32.11 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 34.22 | - |
myo-inositol hexakisphosphate | recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 35.42 | - |
myo-inositol hexakisphosphate | recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C | Aspergillus niger | |
3.1.3.8 | 39.33 | - |
myo-inositol hexakisphosphate | recombinant mutant Q368E/K432R, pH 5.5, 37°C | Aspergillus niger |