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Literature summary extracted from

  • Han, Y.Z.; Zhou, C.-C.; Xu, Y.-Y
    ; Yao, Y.-X.; Chi, Z.; Chi, Z.-M.; Liu, G.-L. High-efficient production of fructo-oligosaccharides from inulin by a two-stage bioprocess using an engineered Yarrowia lipolytica strain (2017), Carbohydr. Polym., 173, 592-599 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.7 gene inuA, recombinant overexpresssion in Yarrowia lipolytica strain Enop56 (engineered from strain Po1h), during fermentation with an inulin concentration of 600 g/l in a 10-l bioreactor, the inulinase activity, fructooligosaccharides (FOS) titer, yield, and productivity reach 551.6 U/ml, 546.6 g/l, 0.91 gFOS/g Inulin, and 15.18 g/l/h, respectively Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus niger O74641
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.7 glycoprotein deglycosylation of the recombinant enzyme by endoglycosidase Endo H Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7 recombinant enzyme from Yarrowia lipolytica strain ENop56 by dialysis and anion exchange chromatography, the enzyme is then deglycosylated by endoglycosidase Endo H, followed by gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 1206.63
-
 purified recombinant enzyme, pH 4.5, 60°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.7 ? x * 56510, deglycosylated recombinant enzyme, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 endo-inulinase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 60
-
 recombinant enzyme Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.7 35 70 maximal activity at 60°C, 70% activity at 35°C, 80% at 50°C and 70°C, profile overview Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.7 30
-
 purified recombinant enzyme, 120 min, 90% activity remaining Aspergillus niger
3.2.1.7 60
-
 purified recombinant enzyme, 120 min, 60% activity remaining Aspergillus niger
3.2.1.7 70
-
 purified recombinant enzyme, 120 min, 10% activity remaining Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 4.5
-
 recombinant enzyme Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.7 3 7 over 70% of maximal activity within this range, profile overview Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.7 2 8 purified recombinant enzyme, 120 min, over 50% activity remaining, completely stable at pH 4.5, profile overview Aspergillus niger