EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.40 | industry | stable alpha-L-rhamnosidase with cleaving terminal alpha-L-rhamnose activity has great potential in industrial application. Enhancing the thermostability of alpha-L-rhamnosidase from Aspergillus terreus makes it a good candidate for industrial processes of isoquercitrin preparation | Aspergillus terreus |
3.2.1.40 | synthesis | adding sorbitol has great potential to promote enzymatic conversion of rutin to isoquercitrin production. Isoquercitrin has several biological activities, including anti-mutagenesis, anti-virus, anti-hypertensive, anti-proliferative effects, lipid peroxidation, oxidative-stress protection as well as other pharmacological effects | Aspergillus terreus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.40 | expression in Pichia pastoris | Aspergillus terreus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 0.481 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, without additives | Aspergillus terreus | |
3.2.1.40 | 0.493 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, in presence of 0.6 M sorbitol | Aspergillus terreus | |
3.2.1.40 | 0.542 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, in presence of 1.0 M sorbitol | Aspergillus terreus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.40 | Aspergillus terreus | I0AZ41 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.40 | - |
Aspergillus terreus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.40 | 4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Aspergillus terreus | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
3.2.1.40 | rutin + H2O | - |
Aspergillus terreus | isoquercitrin + alpha-L-rhamnose | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 65 | - |
half-life: 127.9 min without sorbitol, 5803 min in presence of 2.0 M sorbitol | Aspergillus terreus |
3.2.1.40 | 70 | - |
half-life: 18.2 min without sorbitol, 313.7 min in presence of 2.0 M sorbitol. Half-life and activation free energy are increased by 17.2fold and 8.2 kJ/mol, respectively in presence of 2.0 M sorbitol | Aspergillus terreus |
3.2.1.40 | 75 | - |
half-life: 1.8 min without sorbitol, 54.6 min in presence of 2.0 M sorbitol. 2.0 M sorbitol prolonges the half-life 30.3fold | Aspergillus terreus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 362.5 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, in presence of 1.0 M sorbitol | Aspergillus terreus | |
3.2.1.40 | 379.2 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, in presence of 0.6 M sorbitol | Aspergillus terreus | |
3.2.1.40 | 407.6 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, without additives | Aspergillus terreus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 670 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, in presence of 1.0 M sorbitol | Aspergillus terreus | |
3.2.1.40 | 770 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, in presence of 0.6 M sorbitol | Aspergillus terreus | |
3.2.1.40 | 850 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.5, 65°C, without additives | Aspergillus terreus |