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Literature summary extracted from

  • Song, J.; Kim, S.Y.; Kim, D.H.; Lee, Y.S.; Sim, J.S.; Hahn, B.S.; Lee, C.M.
    Characterization of an inhibitor-resistant endo-1,4-beta-mannanase from the gut microflora metagenome of Hermetia illucens (2018), Biotechnol. Lett., 40, 1377-1387 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 2-mercaptoethanol about 110% activity at 100 mM insect gut metagenome
3.2.1.78 dithiothreitol about 105% activity at 100 mM insect gut metagenome

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Escherichia coli BL21(DE3) cells insect gut metagenome

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 acetonitrile about 55% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 Benzene complete inhibition at 10% (w/v) insect gut metagenome
3.2.1.78 chloroform complete inhibition at 10% (w/v) insect gut metagenome
3.2.1.78 Co2+ about 90% residual activity at 1 mM insect gut metagenome
3.2.1.78 CTAB complete inhibition at 10% (v/v) insect gut metagenome
3.2.1.78 Cyclohexane about 5% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 Fe2+ about 35% residual activity at 1 mM insect gut metagenome
3.2.1.78 Hg2+ complete inhibition at 1 mM insect gut metagenome
3.2.1.78 Mn2+ about 93% residual activity at 1 mM insect gut metagenome
3.2.1.78 additional information EDTA has no effect on enzyme activity insect gut metagenome
3.2.1.78 n-butanol complete inhibition at 10% (w/v) insect gut metagenome
3.2.1.78 n-Heptanol about 5% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 n-hexane about 5% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 n-Hexanol about 5% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 n-Pentanol about 10% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 SDS complete inhibition at 10% (w/v) insect gut metagenome
3.2.1.78 Toluene about 5% residual activity at 10% (w/v) insect gut metagenome
3.2.1.78 Triton X-100 about 55% residual activity at 10% (v/v) insect gut metagenome
3.2.1.78 Tween 20 about 60% residual activity at 10% (v/v) insect gut metagenome
3.2.1.78 Tween 40 about 58% residual activity at 10% (v/v) insect gut metagenome
3.2.1.78 Tween 80 about 61% residual activity at 10% (v/v) insect gut metagenome
3.2.1.78 Urea about 70% residual activity at 100 mM insect gut metagenome

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 additional information not affected by Mg2+, Ca2+, Ni2+, and Cd2+ insect gut metagenome
3.2.1.78 Zn2+ about 105% activity at 1 mM insect gut metagenome

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.78 Acetone the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
3.2.1.78 dimethyl formamide the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
3.2.1.78 dimethyl sulfoxide the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
3.2.1.78 Ethanol the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
3.2.1.78 Methanol the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome
3.2.1.78 propanol the enzyme activity remains stable in the presence of 10% (v/v) insect gut metagenome

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 insect gut metagenome A0A1W6DZL1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 insect gut metagenome

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 TALON metal affinity resin column chromatography insect gut metagenome

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 beta-1,4-mannan + H2O
-
 insect gut metagenome ?
-
 ?
3.2.1.78 guar gum galactomannan + H2O
-
 insect gut metagenome ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 insect gut metagenome ?
-
 ?
3.2.1.78 locust bean gum galactomannan + H2O highest activity insect gut metagenome ?
-
 ?
3.2.1.78 additional information no activity with 4-nitrophenyl beta-D-mannopyranoside insect gut metagenome ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 46000, SDS-PAGE insect gut metagenome

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-1,4-beta-mannanase
-
 insect gut metagenome
3.2.1.78 ManEM17
-
 insect gut metagenome

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 55
-
-
 insect gut metagenome

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 45 60 more than 60% activity between 45 and 60°C insect gut metagenome

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 45 55 below 45°C, the recombinant enzyme remains stable with 95% glycosyl hydrolase activity for 1 h, but quickly loses this function above 55°C insect gut metagenome

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6.5
-
-
 insect gut metagenome

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 5 9.5 long-term (15 h) pre-exposure to acidic and alkaline conditions (pH 5.0-9.5 at 4°C) does not significantly affect enzymatic function, and at least 65% residual activity is retained insect gut metagenome