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Literature summary extracted from

  • Errey, J.C.; Blanchard, J.S.
    Functional annotation and kinetic characterization of PhnO from Salmonella enterica (2006), Biochemistry, 45, 3033-3039 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.280 expression in Escherichia coli BL21 (DE3) star cells Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.280 EDTA
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.280 0.05
-
 malonyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 0.06
-
 acetyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 0.07
-
 propionyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 0.17
-
 (S)-1-aminoethylphosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 1.7
-
 (aminomethyl)phosphonate pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 1.8
-
 2-aminoethyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 4
-
 3-aminopropyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 4.1
-
 1-aminopropyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 12.5
-
 1-aminobutyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 13
-
 4-aminobutylphosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 16.4
-
 2-aminoethanesulfinic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 18.9
-
 2-aminoethanesulfonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.280 Mg2+ the enzyme requires a divalent metal ion for activity. It exhibits a preference for Ni2+ followed by Mn2+ and very poor activity with Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 Mn2+ the enzyme requires a divalent metal ion for activity. It exhibits a preference for Ni2+ followed by Mn2+ and very poor activity with Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 Ni2+ the enzyme requires a divalent metal ion for activity. It exhibits a preference for Ni2+ followed by Mn2+ and very poor activity with Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.280 16499
-
 electrospray ionization-mass spectrometry Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.280 acetyl-CoA + (aminomethyl)phosphonate Salmonella enterica subsp. enterica serovar Typhimurium
-
 CoA + (acetamidomethyl)phosphonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.280 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZKE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.280
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.280 acetyl-CoA + (aminomethyl)phosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + (acetamidomethyl)phosphonate
-
 ?
2.3.1.280 acetyl-CoA + (aminomethyl)phosphonate reaction proceeds via an ordered, sequential kinetic mechanism with acetyl-CoA binding first followed by aminoalkylphosphonate. Attack by the amine on the thioester of AcCoA generates the tetrahedral intermediate that collapses to generate the products Salmonella enterica subsp. enterica serovar Typhimurium CoA + (acetamidomethyl)phosphonate
-
 ?
2.3.1.280 acetyl-CoA + (S)-1-aminoethylphosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 1-aminobutyl phosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 1-aminopropyl phosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 2-aminoethanesulfinic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 2-aminoethanesulfonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 2-aminoethyl phosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 3-aminopropyl phosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 acetyl-CoA + 4-aminobutylphosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 malonyl-CoA + (S)-1-aminoethylphosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?
2.3.1.280 propionyl-CoA + (S)-1-aminoethylphosphonic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.280 phnO
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.280 25
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.280 0.4
-
 malonyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 0.5
-
 4-aminobutylphosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 1
-
 1-aminobutyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 5.4
-
 3-aminopropyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 7
-
 (aminomethyl)phosphonate pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 7.4
-
 1-aminopropyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 9
-
 2-aminoethyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 13.1
-
 acetyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 13.3
-
 (S)-1-aminoethylphosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 14.7
-
 propionyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 14.8
-
 2-aminoethanesulfonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 20
-
 2-aminoethanesulfinic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.280 7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.280 5
-
 2-aminoethyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 120
-
 2-aminoethanesulfinic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 140
-
 3-aminopropyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 180
-
 1-aminopropyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 210
-
 propionyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 220
-
 acetyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 380
-
 4-aminobutylphosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 410
-
 (aminomethyl)phosphonate pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 780
-
 (S)-1-aminoethylphosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 780
-
 2-aminoethanesulfonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 800
-
 1-aminobutyl phosphonic acid pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.280 800
-
 malonyl-CoA cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C Salmonella enterica subsp. enterica serovar Typhimurium