Any feedback?
Literature summary extracted from
- Cloning and expression of mycobacterial glutamine synthetase gene in Escherichia coli (2004), Biochem. Biophys. Res. Commun., 30, 634-638 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | gene MYCTH_2306203, construction of rSt-Phy gene using overlap extension PCR for removal of an intron, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha | Thermothelomyces thermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | additional information | - |
additional information | thermodynamics | Thermothelomyces thermophilus | |
| 3.1.3.8 | 0.156 | - |
myo-inositol hexakisphosphate | native enzyme, pH 5.0, 60°C | Thermothelomyces thermophilus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Thermothelomyces thermophilus | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Thermothelomyces thermophilus ATCC 42464 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Thermothelomyces thermophilus BCRC 31852 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Thermothelomyces thermophilus DSM 1799 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Thermothelomyces thermophilus | G2QH47 | i.e. Myceliophthora thermophila | - |
| 3.1.3.8 | Thermothelomyces thermophilus ATCC 42464 | G2QH47 | i.e. Myceliophthora thermophila | - |
| 3.1.3.8 | Thermothelomyces thermophilus BCRC 31852 | G2QH47 | i.e. Myceliophthora thermophila | - |
| 3.1.3.8 | Thermothelomyces thermophilus DSM 1799 | G2QH47 | i.e. Myceliophthora thermophila | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | recombinant refolded His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis to homogeneity | Thermothelomyces thermophilus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | recombinant enzyme from Escherichia coli inclusion bodies is solubilized by 8 M urea | Thermothelomyces thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 52.4 | - |
purified native enzyme, pH 5.0, 60°C | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Thermothelomyces thermophilus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Thermothelomyces thermophilus ATCC 42464 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Thermothelomyces thermophilus BCRC 31852 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Thermothelomyces thermophilus DSM 1799 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | ? | x * 90000, native enzyme, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | MYCTH_2306203 | locus name | Thermothelomyces thermophilus |
| 3.1.3.8 | St-Phy | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 60 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 60 | - |
purified native enzyme, pH 5.0, has a half-life of 16 h | Thermothelomyces thermophilus |
| 3.1.3.8 | 80 | - |
purified native enzyme, pH 5.0, has a half-life of 1.5 h | Thermothelomyces thermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 37.8 | - |
myo-inositol hexakisphosphate | native enzyme, pH 5.0, 60°C | Thermothelomyces thermophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 5 | - |
- |
Thermothelomyces thermophilus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 242.3 | - |
myo-inositol hexakisphosphate | native enzyme, pH 5.0, 60°C | Thermothelomyces thermophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
