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Literature summary extracted from

  • Dhatwalia, R.; Singh, H.; Reilly, T.J.; Tanner, J.J.
    Crystal structure and tartrate inhibition of Legionella pneumophila histidine acid phosphatase (2015), Arch. Biochem. Biophys., 585, 32-38 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Escherichia coli BL21(AI) cells Legionella pneumophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.2 complexed with the inhibitor L(+)-tartrate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M sodium acetate at pH 4.5 Legionella pneumophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 L-(+)-Tartrate
-
 Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.55
-
 4-nitrophenyl phosphate at pH 5.5 and 37°C Legionella pneumophila
3.1.3.2 3.3
-
 3'-AMP at pH 5.5 and 37°C Legionella pneumophila
3.1.3.2 3.9
-
 5'-AMP at pH 5.5 and 37°C Legionella pneumophila

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Legionella pneumophila Q9APF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 Ni-NTA column chromatography and HiTrap Q column chromatography Legionella pneumophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 3'-AMP + H2O
-
 Legionella pneumophila adenosine + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Legionella pneumophila 4-nitrophenol + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Legionella pneumophila adenosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 HAP
-
 Legionella pneumophila
3.1.3.2 histidine acid phosphatase
-
 Legionella pneumophila
3.1.3.2 major acid phosphatase
-
 Legionella pneumophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 2.4
-
 5'-AMP at pH 5.5 and 37°C Legionella pneumophila
3.1.3.2 3
-
 3'-AMP at pH 5.5 and 37°C Legionella pneumophila
3.1.3.2 16.1
-
 4-nitrophenyl phosphate at pH 5.5 and 37°C Legionella pneumophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0043
-
 L-(+)-Tartrate at pH 5.5 and 37°C Legionella pneumophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.2 0.6
-
 5'-AMP at pH 5.5 and 37°C Legionella pneumophila
3.1.3.2 0.9
-
 3'-AMP at pH 5.5 and 37°C Legionella pneumophila