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Literature summary extracted from

  • Cavalcanti, R.M.F.; Jorge, J.A.; Guimaraes, L.H.S.
    Characterization of Aspergillus fumigatus CAS-21 tannase with potential for propyl gallate synthesis and treatment of tannery effluent from leather industry (2018), 3 Biotech, 8, 270 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 KH2PO4 slightly Aspergillus fumigatus
3.1.1.20 methanol at 1% v/v Aspergillus fumigatus
3.1.1.20 SDS at 1% w/v Aspergillus fumigatus
3.1.1.20 Triton-X100
-
Aspergillus fumigatus
3.1.1.20 Urea about 1.8fold at 4 mM, no effect at 1-3 M Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 2-mercaptoethanol at 1 mM Aspergillus fumigatus
3.1.1.20 acetone at 1% v/v Aspergillus fumigatus
3.1.1.20 acetonitrile at 1% v/v Aspergillus fumigatus
3.1.1.20 Ba2+
-
Aspergillus fumigatus
3.1.1.20 butanol at 1% v/v Aspergillus fumigatus
3.1.1.20 Co2+
-
Aspergillus fumigatus
3.1.1.20 CuSO4
-
Aspergillus fumigatus
3.1.1.20 EDTA at 1 mM Aspergillus fumigatus
3.1.1.20 ethanol at 1% v/v Aspergillus fumigatus
3.1.1.20 Fe2+ at 10 mM Aspergillus fumigatus
3.1.1.20 Fe3+ at 10 mM Aspergillus fumigatus
3.1.1.20 Isopropanol at 1% v/v Aspergillus fumigatus
3.1.1.20 MnCl2
-
Aspergillus fumigatus
3.1.1.20 additional information no or poor inhibition by Ca2+, CuCl2, Hg2+, Mg2+, and Zn2+ Aspergillus fumigatus
3.1.1.20 NaCl
-
Aspergillus fumigatus
3.1.1.20 NH4Cl
-
Aspergillus fumigatus
3.1.1.20 Tween-20 at 0.01% v/v Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 3.61
-
propyl gallate pH 5.0, 35°C Aspergillus fumigatus
3.1.1.20 6.28
-
methyl gallate pH 5.0, 35°C Aspergillus fumigatus
3.1.1.20 6.38
-
Tannic acid pH 5.0, 35°C Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular
-
Aspergillus fumigatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 AgNO3 activates Aspergillus fumigatus
3.1.1.20 MnSO4 activates Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 60340
-
gel filtration Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus fumigatus
-
-
-
3.1.1.20 Aspergillus fumigatus CAS-21
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.20 glycoprotein the enzyme is a glycoprotein with 39.1% carbohydrate content Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 native extracellular enzyme 4.9fold from submerged fermentation broth by anion exchange chromatography, dialysis, and gel filtration, followed by another step of dialysis Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.20 submerged culture
-
Aspergillus fumigatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
Aspergillus fumigatus methanol + gallic acid
-
?
3.1.1.20 methyl gallate + H2O
-
Aspergillus fumigatus CAS-21 methanol + gallic acid
-
?
3.1.1.20 additional information colorimetric actiivty analysis using methanolic rhodanine, spectrometric quantification Aspergillus fumigatus ?
-
?
3.1.1.20 additional information colorimetric actiivty analysis using methanolic rhodanine, spectrometric quantification Aspergillus fumigatus CAS-21 ?
-
?
3.1.1.20 propyl gallate + H2O best substrate Aspergillus fumigatus propanol + gallic acid
-
?
3.1.1.20 propyl gallate + H2O best substrate Aspergillus fumigatus CAS-21 propanol + gallic acid
-
?
3.1.1.20 tannic acid + 1-propanol transesterification Aspergillus fumigatus gallic acid + propyl gallate
-
?
3.1.1.20 tannic acid + 1-propanol transesterification Aspergillus fumigatus CAS-21 gallic acid + propyl gallate
-
?
3.1.1.20 tannic acid + H2O
-
Aspergillus fumigatus gallic acid + ?
-
?
3.1.1.20 tannic acid + H2O
-
Aspergillus fumigatus CAS-21 gallic acid + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 monomer 1 * 60385, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 30 40
-
Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 30 55 over 80% of maximal activity within this range, profile overview Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 30
-
purified native enzyme, maintains 80% of its initial activity when incubated at 30°C for 60 min Aspergillus fumigatus
3.1.1.20 45 50 purified native enzyme, loses 50% activity after 120 min, the half-life is 60 min Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.20 111.93
-
propyl gallate pH 5.0, 35°C Aspergillus fumigatus
3.1.1.20 152.86
-
methyl gallate pH 5.0, 35°C Aspergillus fumigatus
3.1.1.20 362.24
-
Tannic acid pH 5.0, 35°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
-
Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3.5 9 activity range, profile overview Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 5 6 purified exracellular enzyme, 3 h, the enzyme is completely stable and retains 70% of its initial activity after 24 h Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.20 24.34
-
methyl gallate pH 5.0, 35°C Aspergillus fumigatus
3.1.1.20 31
-
propyl gallate pH 5.0, 35°C Aspergillus fumigatus
3.1.1.20 56.78
-
Tannic acid pH 5.0, 35°C Aspergillus fumigatus