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Literature summary extracted from

  • Martinkova, L.; Chmatal, M.
    The integration of cyanide hydratase and tyrosinase catalysts enables effective degradation of cyanide and phenol in coking wastewaters (2016), Water Res., 102, 90-95 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.1 environmental protection the integration of cyanide hydratase and tyrosinase open up new possibilities for the bioremediation of wastewaters with complex pollution. Almost full degradation of free cyanide in the model and the real coking wastewaters is achieved by using a recombinant cyanide hydratase in the first step. The removal of cyanide, a strong inhibitor of tyrosinase, enables an effective degradation of phenols by this enzyme in the second step. Phenol is completely removed from a real coking wastewater within 20 h and cresols are removed by 66% under the same conditions Agaricus bisporus
4.2.1.66 environmental protection the integration of cyanide hydratase and tyrosinase open up new possibilities for the bioremediation of wastewaters with complex pollution. Almost full degradation of free cyanide in the model and the real coking wastewaters is achieved by using a recombinant cyanide hydratase in the first step. The removal of cyanide, a strong inhibitor of tyrosinase, enables an effective degradation of phenols by this enzyme in the second step. Phenol is completely removed from a real coking wastewater within 20 h and cresols are removed by 66% under the same conditions Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 cyanide
-
 Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-
4.2.1.66 Aspergillus niger A9QXE0
-
-
4.2.1.66 Aspergillus niger K10 A9QXE0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 fruit body
-
 Agaricus bisporus
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 25
-
 assay at Agaricus bisporus
4.2.1.66 30
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 7.4
-
 assay at Agaricus bisporus
4.2.1.66 8
-
 assay at Aspergillus niger