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Literature summary extracted from

  • Ochiai, A.; Hashimoto, W.; Murata, K.
    A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58 molecular identification of Atu3025 as an exotype family PL-15 alginate lyase (2006), Res. Microbiol., 157, 642-649 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.B7 expression in Escherichia coli Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.B7 galacturonic acid 1 mM, 93% inhibition Agrobacterium tumefaciens
4.2.2.B7 glucuronic acid 1 mM, 38% inhibition Agrobacterium tumefaciens
4.2.2.B7 Hg2+ 1 mM, 96% inhibition Agrobacterium tumefaciens
4.2.2.B7 additional information not inhibitory: Co2+, Mg2+, Mn2+, dithiothreitol, N-ethylmaleimide, 2-mercaptoethanol, iodoacetic acid, p-chloromercuribenzoate Agrobacterium tumefaciens
4.2.2.B7 Zn2+ 1 mM, 100% inhibition Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.B7 0.0094
-
 alginate pH 7.5, 30°C Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.B7 350000
-
 gel filtration Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.B7 Agrobacterium tumefaciens A9CEJ9
-
-
4.2.2.B7 Agrobacterium tumefaciens C58/ATCC 33970 A9CEJ9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.B7 15.3
-
 substrate poly(alpha-(1,4)-L-guluronate), pH 7.5, 30°C Agrobacterium tumefaciens
4.2.2.B7 16.5
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 7.5, 30°C Agrobacterium tumefaciens
4.2.2.B7 18
-
 substrate poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate), pH 7.5, 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.B7 alginate
-
 Agrobacterium tumefaciens ?
-
 ?
4.2.2.B7 alginate
-
 Agrobacterium tumefaciens C58/ATCC 33970 ?
-
 ?
4.2.2.B7 additional information Atu3025 releases monosaccharides specifically from alginate through a beta-elimination reaction Agrobacterium tumefaciens ?
-
 ?
4.2.2.B7 additional information Atu3025 releases monosaccharides specifically from alginate through a beta-elimination reaction Agrobacterium tumefaciens C58/ATCC 33970 ?
-
 ?
4.2.2.B7 poly(alpha-(1,4)-L-guluronate)
-
 Agrobacterium tumefaciens 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
4.2.2.B7 poly(alpha-(1,4)-L-guluronate)
-
 Agrobacterium tumefaciens C58/ATCC 33970 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
4.2.2.B7 poly(beta-(1,4)-D-mannuronate)
-
 Agrobacterium tumefaciens 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
4.2.2.B7 poly(beta-(1,4)-D-mannuronate)
-
 Agrobacterium tumefaciens C58/ATCC 33970 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
4.2.2.B7 poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate)
-
 Agrobacterium tumefaciens 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
4.2.2.B7 poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate)
-
 Agrobacterium tumefaciens C58/ATCC 33970 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.B7 tetramer 4 * 88000, SDS-PAGE, 4 * 87871, calculated from sequence Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
4.2.2.B7 Atu 3025
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.B7 30
-
-
 Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.B7 7.3
-
-
 Agrobacterium tumefaciens

Expression

EC Number Organism Comment Expression
4.2.2.B7 Agrobacterium tumefaciens expression is induced by growth on low-molecular-weight alginate up