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Literature summary extracted from

  • Matoba, Y.; Yoshida, T.; Izuhara-Kihara, H.; Noda, M.; Sugiyama, M.
    Crystallographic and mutational analyses of cystathionine beta-synthase in the H2S-synthetic gene cluster in Lactobacillus plantarum (2017), Protein Sci., 26, 763-783 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.47 to 2.4 A resolution Lactiplantibacillus plantarum
4.2.1.22 to 2.4 A resolution Lactiplantibacillus plantarum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.47 A70S residue at the substrate binding pocket, important for the H2S-generating activity Lactiplantibacillus plantarum
2.5.1.47 E223G residue at the substrate binding pocket, important for the H2S-generating activity Lactiplantibacillus plantarum
4.2.1.22 A70S residue at the substrate binding pocket, important for the H2S-generating activity Lactiplantibacillus plantarum
4.2.1.22 E223G residue at the substrate binding pocket, important for the H2S-generating activity Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 0.4
-
 L-homocysteine formation of L-cystathionine, mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 0.54
-
 L-homocysteine formation of L-cystathionine, wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 3
-
 L-cysteine formation of L-cystathionine, mutant A70S, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 5.9
-
 L-cysteine formation of L-cystathionine, wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 8.9
-
 L-cysteine formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 20
-
 L-homocysteine formation of L-cystathionine, mutant A70S, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 37
-
 L-cysteine formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 37
-
 L-cysteine formation of L-cystathionine, mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Lactiplantibacillus plantarum F9UT54 bifunctional cystathionine beta-synthase EC 4.2.1.22 and O-acetyl-L-serine sulfhydrylase, EC 2.5.1.47
-
2.5.1.47 Lactiplantibacillus plantarum ATCC BAA-793 F9UT54 bifunctional cystathionine beta-synthase EC 4.2.1.22 and O-acetyl-L-serine sulfhydrylase, EC 2.5.1.47
-
4.2.1.22 Lactiplantibacillus plantarum F9UT54 bifunctional cystathionine beta-synthase EC 4.2.1.22 and O-acetyl-L-serine sulfhydrylase, EC 2.5.1.47
-
4.2.1.22 Lactiplantibacillus plantarum ATCC BAA-793 F9UT54 bifunctional cystathionine beta-synthase EC 4.2.1.22 and O-acetyl-L-serine sulfhydrylase, EC 2.5.1.47
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 2 L-cysteine
-
 Lactiplantibacillus plantarum (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid) + H2S
-
 ?
2.5.1.47 L-cysteine + L-homocysteine
-
 Lactiplantibacillus plantarum L-cystathionine + H2S
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Lactiplantibacillus plantarum L-cysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine + H2O
-
 Lactiplantibacillus plantarum pyruvate + acetate + NH3
-
 ?
2.5.1.47 O-acetyl-L-serine + L-homocysteine
-
 Lactiplantibacillus plantarum cystathionine + acetate
-
 ?
4.2.1.22 2 L-cysteine
-
 Lactiplantibacillus plantarum (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid) + H2S
-
 ?
4.2.1.22 2 L-cysteine
-
 Lactiplantibacillus plantarum ATCC BAA-793 (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid) + H2S
-
 ?
4.2.1.22 L-cysteine + L-homocysteine
-
 Lactiplantibacillus plantarum L-cystathionine + H2S
-
 ?
4.2.1.22 L-cysteine + L-homocysteine
-
 Lactiplantibacillus plantarum ATCC BAA-793 L-cystathionine + H2S
-
 ?
4.2.1.22 O-acetyl-L-serine
-
 Lactiplantibacillus plantarum L-cysteine + acetate
-
 ?
4.2.1.22 O-acetyl-L-serine
-
 Lactiplantibacillus plantarum ATCC BAA-793 L-cysteine + acetate
-
 ?
4.2.1.22 O-acetyl-L-serine + H2O
-
 Lactiplantibacillus plantarum pyruvate + acetate + NH3
-
 ?
4.2.1.22 O-acetyl-L-serine + H2O
-
 Lactiplantibacillus plantarum ATCC BAA-793 pyruvate + acetate + NH3
-
 ?
4.2.1.22 O-acetyl-L-serine + L-homocysteine
-
 Lactiplantibacillus plantarum cystathionine + acetate
-
 ?
4.2.1.22 O-acetyl-L-serine + L-homocysteine
-
 Lactiplantibacillus plantarum ATCC BAA-793 cystathionine + acetate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.22 0.022
-
 L-cysteine formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 0.6
-
 L-cysteine formation of L-cystathionine, mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 1.1
-
 L-cysteine formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 2
-
 L-cysteine formation of L-cystathionine, mutant A70S, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 7.6
-
 L-cysteine formation of L-cystathionine, wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.22 0.0006
-
 L-cysteine formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 0.12
-
 L-cysteine formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 0.15
-
 L-homocysteine formation of L-cystathionine, mutant A70S, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 0.83
-
 L-homocysteine formation of L-cystathionine, mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 1.3
-
 L-cysteine formation of L-cystathionine, wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 1.5
-
 L-cysteine formation of L-cystathionine, mutant A70S, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 8.9
-
 L-cysteine formation of L-cystathionine, mutant E223G, pH 7.5, 37°C Lactiplantibacillus plantarum
4.2.1.22 14
-
 L-homocysteine formation of L-cystathionine, wild-type, pH 7.5, 37°C Lactiplantibacillus plantarum