Literature summary extracted from
Uhl, M.K.; Oberdorfer, G.; Steinkellner, G.; Riegler-Berket, L.; Mink, D.; van Assema, F.; Schuermann, M.; Gruber, K.
The crystal structure of D-threonine aldolase from Alcaligenes xylosoxidans provides insight into a metal ion assisted PLP-dependent mechanism (2015), PLoS ONE, 10, e0124056 .
Application
EC Number |
Application |
Comment |
Organism |
---|
4.1.2.42 |
pharmacology |
the enzyme has a considerable potential in biocatalysis for the stereospecific synthesis of various beta-hydroxy amino acids, which are valuable building blocks for the production of pharmaceuticals |
Achromobacter xylosoxidans |
4.1.2.42 |
synthesis |
the enzyme has a considerable potential in biocatalysis for the stereospecific synthesis of various beta-hydroxy amino acids, which are valuable building blocks for the production of pharmaceuticals |
Achromobacter xylosoxidans |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.2.42 |
expression in Escherichia coli BL21 Star (DE3) cells |
Achromobacter xylosoxidans |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.2.42 |
determination of the crystal structure at 1.5 A resolution |
Achromobacter xylosoxidans |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.2.42 |
Mn2+ |
the role of the essential metal (manganese) ion is most likely to activate the beta-hydroxy group of the substrate for deprotonation by His193 |
Achromobacter xylosoxidans |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.2.42 |
Achromobacter xylosoxidans |
A0A0J9X243 |
IFO 12669 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.2.42 |
- |
Achromobacter xylosoxidans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.2.42 |
D-threonine |
- |
Achromobacter xylosoxidans |
glycine + acetaldehyde |
- |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.2.42 |
pyridoxal 5'-phosphate |
pyridoxal 5'-phosphate dependent enzyme. The metal binding siteis close to the pyridoxal 5'-phosphate cofactor in the active site of the enzyme. Metal ion assisted pyridoxal 5'-phosphate-dependent mechanism |
Achromobacter xylosoxidans |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.1.2.42 |
physiological function |
the enzyme plays a major role in degradation of D-threonine |
Achromobacter xylosoxidans |