Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nieuwenhuizen, N.J.; Chen, X.; Wang, M.Y.; Matich, A.J.; Perez, R.L.; Allan, A.C.; Green, S.A.; Atkinson, R.G.
    Natural variation in monoterpene synthesis in kiwifruit transcriptional regulation of terpene synthases by NAC and ethylene-insensitive3-like transcription factors (2015), Plant Physiol., 167, 1243-1258 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.113 gene TPS1, Actinidia chinensis terpene synthase1 (AcTPS1) is identified as part of an array of eight tandemly duplicated genes, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinidia chinensis
4.2.3.113 gene TPS1, phylogenetic analysis, quantitative PCR enzyme expression analysis, comparative promoter analysis identifies potential NAC for no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), and cup-shaped cotyledon (CUC)-domain transcription factor and ethylene-insensitive3-like transcription factor (TF) binding sites in the AaTPS1 promoter, and cloned members of both TF classes are able to activate the AaTPS1 promoter in transient assays. AaNAC2, AaNAC3, and AaNAC4 bind a 28-bp fragment of the proximal NAC binding site in the AaTPS1 promoter but not the Actinidia chinensis AcTPS1 promoter, where the NAC binding site is mutated, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3). Transgenic tobacco leaves expressing AcTPS1 produce beta-myrcene in low amounts as the only gene-specific product Actinidia arguta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.113 chloroplast
-
 Actinidia arguta 9507
-
4.2.3.113 chloroplast
-
 Actinidia chinensis 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.113 Mg2+ activates, Km is 1.5 mM Actinidia arguta
4.2.3.113 Mg2+ activates, Km is 1.5 mM Actinidia chinensis
4.2.3.113 Mn2+ activates, preferred, Km is 0.020 mM Actinidia arguta
4.2.3.113 Mn2+ activates, preferred, Km is 0.020 mM Actinidia chinensis
4.2.3.113 additional information requirement for a divalent metal ion cofactor. AaTPS1 favors Mn2+ over Mg2+ and shows a 3fold increase in Vmax with Mn2+ compared to Mg2+. K+ has no effect the enzyme activity Actinidia chinensis
4.2.3.113 additional information requirement for a divalent metal ion cofactor. AcTPS1 favors Mn2+ over Mg2+ and shows a 2fold increase in Vmax with Mn2+ compared to Mg2+. K+ has no effect the enzyme activity Actinidia arguta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.113 geranyl diphosphate Actinidia arguta
-
 terpinolene + diphosphate
-
 ?
4.2.3.113 geranyl diphosphate Actinidia chinensis
-
 terpinolene + diphosphate
-
 ?
4.2.3.113 additional information Actinidia chinensis the enantiomeric composition of terpenes produced by AaTPS1 in vitro and those produced in ripe Actinidia arguta cv. Hortgem Tahi fruit (stage a3/a4) are very similar ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.113 Actinidia arguta A0A075EAR1 cv. Hortgem Tahi
-
4.2.3.113 Actinidia chinensis A0A075EB43 cv. Hort16A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.113 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to over 95% purity Actinidia arguta
4.2.3.113 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to over 95% purity Actinidia chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.113 fruit ripe, AaTPS1 expression increases sharply during fruit ripening, with the highest levels of expression being found in overripe fruit Actinidia arguta
-
4.2.3.113 fruit ripe, gene AcTPS1 expression and terpene production are observed only at low levels in developing fruit. AaTPS1 expression increases sharply during fruit ripening, with the highest levels of expression being found in overripe fruit Actinidia chinensis
-
4.2.3.113 additional information rates of volatile terpene release by Actinidia arguta cv. Hortgem Tahi and Actinidia chinensis cv. Hort16A during fruit development and ripening Actinidia arguta
-
4.2.3.113 additional information rates of volatile terpene release by Actinidia arguta cv. Hortgem Tahi and Actinidia chinensis cv. Hort16A during fruit development and ripening Actinidia chinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.113 geranyl diphosphate
-
 Actinidia arguta terpinolene + diphosphate
-
 ?
4.2.3.113 geranyl diphosphate
-
 Actinidia chinensis terpinolene + diphosphate
-
 ?
4.2.3.113 additional information the enantiomeric composition of terpenes produced by AaTPS1 in vitro and those produced in ripe Actinidia arguta cv. Hortgem Tahi fruit (stage a3/a4) are very similar Actinidia chinensis ?
-
 ?
4.2.3.113 additional information AcTPS1 produces the noncyclic monoterpenes geraniol and beta-myrcene as the major products rather than 1,8-cineole, which is the predominant terpene associated with Actinidia chinensis fruit. Transgenic tobacco leaves expressing AcTPS1 produce beta-myrcene in low amounts as the only gene-specific product. Farnesyl diphosphate is no substrate Actinidia arguta ?
-
 ?
4.2.3.113 additional information the recombinant AaTPS1 enzyme catalyzes the conversion of geranyl diphosphate to both cyclic and noncyclic monoterpene products, kinetics, overview. Specifically, alpha-terpinolene is the predominant terpene produced, accounting for approximately 67% of the total monoterpenes, followed by beta-myrcene (10%), predominantly (S)-limonene (9%), and smaller amounts of alpha-pinene, linalool, and alpha-terpineol. Farnesyl diphosphate is no substrate Actinidia chinensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.113 AaTPS1
-
 Actinidia arguta
4.2.3.113 AcTPS1
-
 Actinidia chinensis
4.2.3.113 terpene synthase1
-
 Actinidia chinensis
4.2.3.113 TPS1
-
 Actinidia arguta
4.2.3.113 TPS1
-
 Actinidia chinensis

General Information

EC Number General Information Comment Organism
4.2.3.113 evolution Actinidia arguta AaTPS1 and Actinidia chinensis AcTPS1 likely originate from a common ancestral gene, phylogenetic analysis of the AaTPS1 and AcTPS1 Actinidia arguta
4.2.3.113 evolution Actinidia chinensis terpene synthase1 (AcTPS1) is identified as part of an array of eight tandemly duplicated genes. Actinidia arguta AaTPS1 and Actinidia chinensis AcTPS1 likely originate from a common ancestral gene, phylogenetic analysis of the AaTPS1 and AcTPS1 Actinidia chinensis
4.2.3.113 malfunction transcription factors AaNAC2, AaNAC3, and AaNAC4 bind a 28-bp fragment of the proximal NAC binding site in the Actinidia arguta TPS1 promoter but not the Actinidia chinensis AcTPS1 promoter, where the NAC binding site is mutated. Activation can be restored by reintroducing multiple repeats of the 12-bp NAC core-binding motif. The absence of NAC transcriptional activation in ripe Actinidia chinensis fruit can account for the low accumulation of AcTPS1 transcript, protein, and monoterpene volatiles in this species Actinidia chinensis
4.2.3.113 physiological function high rates of terpinolene production in ripe Actinidia arguta fruit are correlated with increasing gene and protein expression of Actinidia arguta terpene synthase1 (AaTPS1) and correlates with an increase in transcript levels of the 2-C-methyl-D-erythritol 4-phosphate pathway enzyme 1-deoxy-D-xylulose-5-phosphate synthase (DXS). DXS is likely to be the key step in regulating 2-C-methyl-D-erythritol 4-phosphate substrate flux in kiwifruit. Transcription factors AaNAC2, AaNAC3, and AaNAC4 bind a 28-bp fragment of the proximal NAC binding site in the AaTPS1 promoter but not the Actinidia chinensis AcTPS1 promoter, where the NAC binding site is mutated. Importance of NAC transcription factors in controlling monoterpene production and other traits in ripening fruits. Ripe fruits of Actinidia arguta have a very high amount of terpene volatiles released as compared to other Actinidia species fruits, overview Actinidia arguta
4.2.3.113 physiological function high rates of terpinolene production in ripe Actinidia arguta fruit are correlated with increasing gene and protein expression of Actinidia arguta terpene synthase1 (AaTPS1) and correlates with an increase in transcript levels of the 2-C-methyl-D-erythritol 4-phosphate pathway enzyme 1-deoxy-D-xylulose-5-phosphate synthase (DXS). Importance of NAC transcription factors in controlling monoterpene production and other traits in ripening fruits Actinidia chinensis