Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vanegas, K.G.; Larsen, A.B.; Eichenberger, M.; Fischer, D.; Mortensen, U.H.; Naesby, M.
    Indirect and direct routes to C-glycosylated flavones in Saccharomyces cerevisiae (2018), Microb. Cell Fact., 17, 107 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.162 synthesis two-step indirect glycosylation using combinations of flavanone 2-hydroxylase and C-glycosyltransferases, to convert 2-hydroxyflavanone intermediates into the 6C-glucoside flavones isovitexin and isoorientin, and the 8C-glucoside flavones vitexin and orientin. The ratio between 6C and 8C glycosylation depended on the C-glycosyltransferase used. The indirect route resuls in mixtures, similar to what has been reported for in vitro experiments Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.162 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
-
 2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.162 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
-
 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.162 Oryza sativa Japonica Group Q5VRI5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.162 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group 2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.162 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.162 CYP93G2
-
 Oryza sativa Japonica Group
1.14.14.162 OsF2H
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.162 cytochrome P-450 a cytochrome P-450 (heme thiolate) enzyme Oryza sativa Japonica Group