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Literature summary extracted from

  • Namitha, K.; Negi, P.
    Transformation of tomato cv. Arka Ahuti (Solanum lycopersicum L.) with phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) genes increases carotenoid content and antioxidant potential (2018), J. Plant Biochem. Biotechnol., 27, 68-77 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.31 gene crtI, recombinant expression of the enzyme driven by 35S promoter in via Agrobacterium tumefaciens strain GV 3101 containing binary vector pRI 101(I) in fruits of Solanum tuberosum, coexpression of genes CrtI and CrtY, the latter encoding lycopene beta-cyclase, in transgenic tomato fruits, the method used is agroinjection of tomato fruits, a method of directly injecting Agrobacterium containing transgenes into fruits Pantoea agglomerans
5.5.1.19 expressed in fruits of Solanum lycopersicum cultivar Arka Ahuti Pantoea agglomerans

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.31 additional information transformation of Solanum lycopersicum cv. Arka Ahuti with phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) genes increases carotenoid content and antioxidant potential. coexpression of genes CrtI and CrtY, the latter encoding lycopene beta-cyclase, in transgenic tomato fruits, the method used is agroinjection of tomato fruits, a method of directly injecting Agrobacterium containing transgenes into fruits. The lycopene and beta-carotene content in green house grown tomatoes show a gradual increase from BR3 to BR7 stage, whereas in open-field grown tomatoes, the gradual increase is observed from BR3 to BR10 stage. Comparison of total antioxidant capacity of control and transformed tomatoes evaluated by phosphomolybdenum methodreveals higher total antioxidant capacity in the hydrophilic and lipophilic hexane as well as lipophilic acetone extracts in tomatoes with enhanced carotene content (CrtI and CrtY) fruits as compared to control tomato samples (GHC and OC), phenotypes, overview Pantoea agglomerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.31 15-cis-phytoene + acceptor Pantoea agglomerans
-
 all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.31 all-trans-neurosporene + acceptor Pantoea agglomerans
-
 all-trans-lycopene + reduced acceptor
-
 ?
1.3.99.31 all-trans-phytofluene + acceptor Pantoea agglomerans
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.31 all-trans-zeta-carotene + acceptor Pantoea agglomerans
-
 all-trans-neurosporene + reduced acceptor
-
 ?
5.5.1.19 lycopene Pantoea agglomerans
-
 beta-carotene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.31 Pantoea agglomerans Q206M4 Erwinia herbicola or Pantoea agglomerans
-
5.5.1.19 Pantoea agglomerans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.31 15-cis-phytoene + acceptor
-
 Pantoea agglomerans all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.31 all-trans-neurosporene + acceptor
-
 Pantoea agglomerans all-trans-lycopene + reduced acceptor
-
 ?
1.3.99.31 all-trans-phytofluene + acceptor
-
 Pantoea agglomerans all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.31 all-trans-zeta-carotene + acceptor
-
 Pantoea agglomerans all-trans-neurosporene + reduced acceptor
-
 ?
5.5.1.19 lycopene
-
 Pantoea agglomerans beta-carotene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.31 CrtI
-
 Pantoea agglomerans
1.3.99.31 phytoene desaturase
-
 Pantoea agglomerans
5.5.1.19 CrtY
-
 Pantoea agglomerans